Restriction map for
pF1KB5799
(Linear) (Six-base) MAPSORT of: KIBB5799.seq Check: 5297 from: 1 to: 2502
KIBB5799.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 0 869 1592 2502
Size: 869 723 910
Fragments arranged by size:
910 869 723
AccI GT'mk_AC
Cuts at: 0 1883 2502
Size: 1883 619
AclI AA'CG_TT
Cuts at: 0 2456 2502
Size: 2456 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 763 2502
Size: 580 183 1739
Fragments arranged by size:
1739 580 183
AflIII A'CryG_T
Cuts at: 0 823 2502
Size: 823 1679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1867 2502
Size: 1867 635
AlwNI CAG_nnn'CTG
Cuts at: 0 897 915 995 1536 1998 2502
Size: 897 18 80 541 462 504
Fragments arranged by size:
897 541 504 462 80 18
ApaI G_GGCC'C
Cuts at: 0 97 2165 2502
Size: 97 2068 337
Fragments arranged by size:
2068 337 97
ApoI r'AATT_y
Cuts at: 0 168 637 1901 2014 2502
Size: 168 469 1264 113 488
Fragments arranged by size:
1264 488 469 168 113
AseI AT'TA_AT
Cuts at: 0 1388 2502
Size: 1388 1114
AvaI C'yCGr_G
Cuts at: 0 1559 1616 2502
Size: 1559 57 886
Fragments arranged by size:
1559 886 57
BanII G_rGCy'C
Cuts at: 0 97 755 851 2048 2165 2502
Size: 97 658 96 1197 117 337
Fragments arranged by size:
1197 658 337 117 97 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1230 2208 2502
Size: 605 625 978 294
Fragments arranged by size:
978 625 605 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2224 2258 2502
Size: 2224 34 244
Fragments arranged by size:
2224 244 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 169 2502
Size: 169 2333
BclI T'GATC_A
Cuts at: 0 1820 2502
Size: 1820 682
BfrBI ATG'CAT
Cuts at: 0 1462 2502
Size: 1462 1040
BglI GCCn_nnn'nGGC
Cuts at: 0 2160 2502
Size: 2160 342
BlpI GC'TnA_GC
Cuts at: 0 754 2502
Size: 754 1748
Bme1580I G_kGCm'C
Cuts at: 0 97 1029 1443 1710 1724 2165 2502
Size: 97 932 414 267 14 441 337
Fragments arranged by size:
932 441 414 337 267 97 14
BmgBI CAC'GTC
Cuts at: 0 826 2502
Size: 826 1676
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 285 1235 2502
Size: 79 206 950 1267
Fragments arranged by size:
1267 950 206 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 725 2502
Size: 725 1777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 439 1294 2502
Size: 247 192 855 1208
Fragments arranged by size:
1208 855 247 192
Bpu10I CC'TnA_GC
Cuts at: 0 808 2502
Size: 808 1694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1617 2502
Size: 238 1379 885
Fragments arranged by size:
1379 885 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 39 2182 2325 2502
Size: 39 2143 143 177
Fragments arranged by size:
2143 177 143 39
BsaAI yAC'GTr
Cuts at: 0 2086 2502
Size: 2086 416
BsaHI Gr'CG_yC
Cuts at: 0 470 866 1589 1886 2502
Size: 470 396 723 297 616
Fragments arranged by size:
723 616 470 396 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 422 452 1561 1591 2502
Size: 422 30 1109 30 911
Fragments arranged by size:
1109 911 422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 124 127 139 142 145 148 520
Size: 51 73 3 12 3 3 3 372
Cuts at: 520 1432 1876 2502
Size: 912 444 626
Fragments arranged by size:
912 626 444 372 73 51 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 73 1467 2281 2502
Size: 73 1394 814 221
Fragments arranged by size:
1394 814 221 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1411 1462 1973 2191 2502
Size: 1096 315 51 511 218 311
Fragments arranged by size:
1096 511 315 311 218 51
BsiHKAI G_wGCw'C
Cuts at: 0 365 546 851 2069 2502
Size: 365 181 305 1218 433
Fragments arranged by size:
1218 433 365 305 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1495 2502
Size: 1495 1007
Bsp1286I G_dGCh'C
Cuts at: 0 97 365 546 755 851 1029 1443 1710
Size: 97 268 181 209 96 178 414 267
Cuts at: 1710 1724 2048 2069 2165 2502
Size: 14 324 21 96 337
Fragments arranged by size:
414 337 324 268 267 209 181 178
97 96 96 21 14
BspHI T'CATG_A
Cuts at: 0 834 1943 2462 2502
Size: 834 1109 519 40
Fragments arranged by size:
1109 834 519 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 990 1118 1484 2502
Size: 990 128 366 1018
Fragments arranged by size:
1018 990 366 128
BsrBI CCG'CTC
Cuts at: 0 1052 2502
Size: 1052 1450
BsrDI GCAATG_nn'
Cuts at: 0 185 1085 1462 2502
Size: 185 900 377 1040
Fragments arranged by size:
1040 900 377 185
BsrGI T'GTAC_A
Cuts at: 0 722 1019 2502
Size: 722 297 1483
Fragments arranged by size:
1483 722 297
BssSI C'ACGA_G
Cuts at: 0 244 1541 2306 2502
Size: 244 1297 765 196
Fragments arranged by size:
1297 765 244 196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1455 2037 2502
Size: 1455 582 465
Fragments arranged by size:
1455 582 465
BstYI r'GATC_y
Cuts at: 0 146 1197 1487 2436 2502
Size: 146 1051 290 949 66
Fragments arranged by size:
1051 949 290 146 66
Bsu36I CC'TnA_GG
Cuts at: 0 9 1036 2502
Size: 9 1027 1466
Fragments arranged by size:
1466 1027 9
BtgI C'CryG_G
Cuts at: 0 1361 2183 2502
Size: 1361 822 319
Fragments arranged by size:
1361 822 319
BtsI GCAGTG_nn'
Cuts at: 0 1311 1479 1979 1985 2343 2502
Size: 1311 168 500 6 358 159
Fragments arranged by size:
1311 500 358 168 159 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 607 2502
Size: 607 1895
EaeI y'GGCC_r
Cuts at: 0 140 731 2057 2114 2502
Size: 140 591 1326 57 388
Fragments arranged by size:
1326 591 388 140 57
EarI CTCTTCn'nnn_
Cuts at: 0 502 510 1786 2502
Size: 502 8 1276 716
Fragments arranged by size:
1276 716 502 8
EcoICRI GAG'CTC
Cuts at: 0 849 2502
Size: 849 1653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 439 580 763 1294 2502
Size: 247 192 141 183 531 1208
Fragments arranged by size:
1208 531 247 192 183 141
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1252 1807 1957 2502
Size: 1252 555 150 545
Fragments arranged by size:
1252 555 545 150
EcoO109I rG'GnC_Cy
Cuts at: 0 94 1473 2161 2162 2502
Size: 94 1379 688 1 340
Fragments arranged by size:
1379 688 340 94 1
EcoRI G'AATT_C
Cuts at: 0 168 1901 2502
Size: 168 1733 601
Fragments arranged by size:
1733 601 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 903 935 1535 1561 1567 1593
Size: 371 32 500 32 600 26 6 26
Cuts at: 1593 2502
Size: 909
Fragments arranged by size:
909 600 500 371 32 32 26 26
6
HincII GTy'rAC
Cuts at: 0 647 2502
Size: 647 1855
HindIII A'AGCT_T
Cuts at: 0 256 346 2296 2502
Size: 256 90 1950 206
Fragments arranged by size:
1950 256 206 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 2094 2502
Size: 853 1241 408
Fragments arranged by size:
1241 853 408
MscI TGG'CCA
Cuts at: 0 733 2116 2502
Size: 733 1383 386
Fragments arranged by size:
1383 733 386
MslI CAynn'nnrTG
Cuts at: 0 155 1828 1942 2110 2502
Size: 155 1673 114 168 392
Fragments arranged by size:
1673 392 168 155 114
MspA1I CmG'CkG
Cuts at: 0 1549 2062 2272 2502
Size: 1549 513 210 230
Fragments arranged by size:
1549 513 230 210
NcoI C'CATG_G
Cuts at: 0 1361 2183 2502
Size: 1361 822 319
Fragments arranged by size:
1361 822 319
NsiI A_TGCA'T
Cuts at: 0 1464 2502
Size: 1464 1038
NspI r_CATG'y
Cuts at: 0 2099 2502
Size: 2099 403
PasI CC'CwG_GG
Cuts at: 0 98 1029 2502
Size: 98 931 1473
Fragments arranged by size:
1473 931 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1536 2502
Size: 897 639 966
Fragments arranged by size:
966 897 639
PfoI T'CCnGG_A
Cuts at: 0 1490 2502
Size: 1490 1012
PmlI CAC'GTG
Cuts at: 0 2086 2502
Size: 2086 416
PpuMI rG'GwC_Cy
Cuts at: 0 1473 2502
Size: 1473 1029
PspOMI G'GGCC_C
Cuts at: 0 93 2161 2502
Size: 93 2068 341
Fragments arranged by size:
2068 341 93
PvuII CAG'CTG
Cuts at: 0 1549 2272 2502
Size: 1549 723 230
Fragments arranged by size:
1549 723 230
SacI G_AGCT'C
Cuts at: 0 851 2502
Size: 851 1651
SapI GCTCTTCn'nnn_
Cuts at: 0 1786 2502
Size: 1786 716
SexAI A'CCwGG_T
Cuts at: 0 1958 2502
Size: 1958 544
SfcI C'TryA_G
Cuts at: 0 688 1522 2502
Size: 688 834 980
Fragments arranged by size:
980 834 688
SmaI CCC'GGG
Cuts at: 0 1618 2502
Size: 1618 884
SmlI C'TyrA_G
Cuts at: 0 253 1632 2502
Size: 253 1379 870
Fragments arranged by size:
1379 870 253
SpeI A'CTAG_T
Cuts at: 0 202 2502
Size: 202 2300
StyI C'CwwG_G
Cuts at: 0 22 983 1361 1448 1659 1926 2183 2502
Size: 22 961 378 87 211 267 257 319
Fragments arranged by size:
961 378 319 267 257 211 87 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 2502
Size: 57 2445
TatI w'GTAC_w
Cuts at: 0 722 1019 2502
Size: 722 297 1483
Fragments arranged by size:
1483 722 297
XmaI C'CCGG_G
Cuts at: 0 1616 2502
Size: 1616 886
XmnI GAAnn'nnTTC
Cuts at: 0 2458 2502
Size: 2458 44
ZraI GAC'GTC
Cuts at: 0 867 1590 2502
Size: 867 723 912
Fragments arranged by size:
912 867 723
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AlfI AlwNI ApaI
ApoI AseI AvaI BanII BbsI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NcoI NsiI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PvuII
SacI SapI SexAI SfcI SmaI SmlI SpeI StyI
TaqII TatI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BglII BmtI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BsrFI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI