(Linear) (Six-base) MAPSORT of: KIBB5808.seq Check: 8148 from: 1 to: 1362 KIBB5808.orf With 182 enzymes: SgfI * October 7, 2009 14:17 .. AccI GT'mk_AC Cuts at: 0 257 628 1362 Size: 257 371 734 Fragments arranged by size: 734 371 257 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1219 1362 Size: 1219 143 AflIII A'CryG_T Cuts at: 0 371 373 1121 1362 Size: 371 2 748 241 Fragments arranged by size: 748 371 241 2 AlwNI CAG_nnn'CTG Cuts at: 0 954 1362 Size: 954 408 ApaI G_GGCC'C Cuts at: 0 917 1362 Size: 917 445 AvaI C'yCGr_G Cuts at: 0 251 1362 Size: 251 1111 BanI G'GyrC_C Cuts at: 0 34 219 1097 1362 Size: 34 185 878 265 Fragments arranged by size: 878 265 185 34 BanII G_rGCy'C Cuts at: 0 130 550 566 761 917 1040 1362 Size: 130 420 16 195 156 123 322 Fragments arranged by size: 420 322 195 156 130 123 16 BbeI G_GCGC'C Cuts at: 0 1101 1362 Size: 1101 261 BglI GCCn_nnn'nGGC Cuts at: 0 620 1362 Size: 620 742 BlpI GC'TnA_GC Cuts at: 0 124 544 760 991 1362 Size: 124 420 216 231 371 Fragments arranged by size: 420 371 231 216 124 Bme1580I G_kGCm'C Cuts at: 0 39 96 224 917 1362 Size: 39 57 128 693 445 Fragments arranged by size: 693 445 128 57 39 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 192 1290 1362 Size: 192 1098 72 Fragments arranged by size: 1098 192 72 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 624 982 1051 1267 1362 Size: 624 358 69 216 95 Fragments arranged by size: 624 358 216 95 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 110 1010 1362 Size: 110 900 352 Fragments arranged by size: 900 352 110 BsaAI yAC'GTr Cuts at: 0 374 1362 Size: 374 988 BsaHI Gr'CG_yC Cuts at: 0 1098 1362 Size: 1098 264 BsaWI w'CCGG_w Cuts at: 0 488 884 1362 Size: 488 396 478 Fragments arranged by size: 488 478 396 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 226 239 1048 1266 1362 Size: 226 13 809 218 96 Fragments arranged by size: 809 226 218 96 13 BseYI C'CCAG_C Cuts at: 0 223 618 943 1108 1356 1362 Size: 223 395 325 165 248 6 Fragments arranged by size: 395 325 248 223 165 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 679 810 1362 Size: 665 14 131 552 Fragments arranged by size: 665 552 131 14 BsiHKAI G_wGCw'C Cuts at: 0 202 566 1362 Size: 202 364 796 Fragments arranged by size: 796 364 202 Bsp1286I G_dGCh'C Cuts at: 0 39 96 130 202 224 550 566 761 Size: 39 57 34 72 22 326 16 195 Cuts at: 761 917 1040 1362 Size: 156 123 322 Fragments arranged by size: 326 322 195 156 123 72 57 39 34 22 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 58 1362 Size: 58 1304 BsrDI GCAATG_nn' Cuts at: 0 285 764 1362 Size: 285 479 598 Fragments arranged by size: 598 479 285 BsrFI r'CCGG_y Cuts at: 0 434 904 1100 1362 Size: 434 470 196 262 Fragments arranged by size: 470 434 262 196 BsrGI T'GTAC_A Cuts at: 0 368 1362 Size: 368 994 BstAPI GCAn_nnn'nTGC Cuts at: 0 954 1362 Size: 954 408 BstBI TT'CG_AA Cuts at: 0 452 1362 Size: 452 910 BstEII G'GTnAC_C Cuts at: 0 408 524 1362 Size: 408 116 838 Fragments arranged by size: 838 408 116 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 1362 Size: 171 1191 BstYI r'GATC_y Cuts at: 0 49 666 1362 Size: 49 617 696 Fragments arranged by size: 696 617 49 BstZ17I GTA'TAC Cuts at: 0 629 1362 Size: 629 733 Bsu36I CC'TnA_GG Cuts at: 0 1081 1362 Size: 1081 281 BtsI GCAGTG_nn' Cuts at: 0 1066 1362 Size: 1066 296 DraIII CAC_nnn'GTG Cuts at: 0 657 1362 Size: 657 705 EaeI y'GGCC_r Cuts at: 0 2 1362 Size: 2 1360 EarI CTCTTCn'nnn_ Cuts at: 0 554 1362 Size: 554 808 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 98 233 1362 Size: 98 135 1129 Fragments arranged by size: 1129 135 98 EcoICRI GAG'CTC Cuts at: 0 564 1362 Size: 564 798 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 624 982 1051 1219 1267 1362 Size: 624 358 69 168 48 95 Fragments arranged by size: 624 358 168 95 69 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 163 1362 Size: 163 1199 EcoO109I rG'GnC_Cy Cuts at: 0 43 141 914 1344 1362 Size: 43 98 773 430 18 Fragments arranged by size: 773 430 98 43 18 EcoRV GAT'ATC Cuts at: 0 807 1362 Size: 807 555 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 285 354 1362 Size: 285 69 1008 Fragments arranged by size: 1008 285 69 HaeII r_GCGC'y Cuts at: 0 998 1101 1362 Size: 998 103 261 Fragments arranged by size: 998 261 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 162 194 274 306 402 434 1261 1293 Size: 162 32 80 32 96 32 827 32 Cuts at: 1293 1362 Size: 69 Fragments arranged by size: 827 162 96 80 69 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1097 1362 Size: 1097 265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 890 1362 Size: 890 472 MscI TGG'CCA Cuts at: 0 4 1362 Size: 4 1358 MslI CAynn'nnrTG Cuts at: 0 1298 1362 Size: 1298 64 MspA1I CmG'CkG Cuts at: 0 618 663 1362 Size: 618 45 699 Fragments arranged by size: 699 618 45 NaeI GCC'GGC Cuts at: 0 436 906 1102 1362 Size: 436 470 196 260 Fragments arranged by size: 470 436 260 196 NarI GG'CG_CC Cuts at: 0 1098 1362 Size: 1098 264 NgoMIV G'CCGG_C Cuts at: 0 434 904 1100 1362 Size: 434 470 196 262 Fragments arranged by size: 470 434 262 196 NspI r_CATG'y Cuts at: 0 465 1125 1362 Size: 465 660 237 Fragments arranged by size: 660 465 237 PasI CC'CwG_GG Cuts at: 0 39 61 88 1362 Size: 39 22 27 1274 Fragments arranged by size: 1274 39 27 22 PciI A'CATG_T Cuts at: 0 1121 1362 Size: 1121 241 PmlI CAC'GTG Cuts at: 0 374 1362 Size: 374 988 PspOMI G'GGCC_C Cuts at: 0 913 1362 Size: 913 449 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 352 384 1362 Size: 352 32 978 Fragments arranged by size: 978 352 32 PstI C_TGCA'G Cuts at: 0 1061 1362 Size: 1061 301 PvuII CAG'CTG Cuts at: 0 618 663 1362 Size: 618 45 699 Fragments arranged by size: 699 618 45 SacI G_AGCT'C Cuts at: 0 566 1362 Size: 566 796 SapI GCTCTTCn'nnn_ Cuts at: 0 554 1362 Size: 554 808 ScaI AGT'ACT Cuts at: 0 430 1362 Size: 430 932 SfcI C'TryA_G Cuts at: 0 439 1057 1362 Size: 439 618 305 Fragments arranged by size: 618 439 305 SfoI GGC'GCC Cuts at: 0 1099 1362 Size: 1099 263 SgrAI Cr'CCGG_yG Cuts at: 0 1100 1362 Size: 1100 262 StyI C'CwwG_G Cuts at: 0 164 467 572 1129 1362 Size: 164 303 105 557 233 Fragments arranged by size: 557 303 233 164 105 TatI w'GTAC_w Cuts at: 0 368 428 1362 Size: 368 60 934 Fragments arranged by size: 934 368 60 Tth111I GACn'n_nGTC Cuts at: 0 928 1362 Size: 928 434 XmnI GAAnn'nnTTC Cuts at: 0 448 1362 Size: 448 914 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI AvaI BanI BanII BbeI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PmlI PspOMI PsrI PstI PvuII SacI SapI ScaI SfcI SfoI SgrAI StyI TatI Tth111I XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI