Restriction map for
pF1KB5811
(Linear) (Six-base) MAPSORT of: KIBB5811.seq Check: 7349 from: 1 to: 1422
KIBB5811.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 0 348 1132 1422
Size: 348 784 290
Fragments arranged by size:
784 348 290
AccI GT'mk_AC
Cuts at: 0 301 1422
Size: 301 1121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 1030 1158 1277 1422
Size: 552 478 128 119 145
Fragments arranged by size:
552 478 145 128 119
AfeI AGC'GCT
Cuts at: 0 775 1422
Size: 775 647
AflII C'TTAA_G
Cuts at: 0 1137 1422
Size: 1137 285
AhdI GACnn_n'nnGTC
Cuts at: 0 1395 1422
Size: 1395 27
AleI CACnn'nnGTG
Cuts at: 0 1348 1422
Size: 1348 74
ApoI r'AATT_y
Cuts at: 0 143 1422
Size: 143 1279
AvaI C'yCGr_G
Cuts at: 0 1376 1422
Size: 1376 46
BanII G_rGCy'C
Cuts at: 0 413 1422
Size: 413 1009
BbsI GAAGACnn'nnnn_
Cuts at: 0 279 1132 1422
Size: 279 853 290
Fragments arranged by size:
853 290 279
BglII A'GATC_T
Cuts at: 0 695 1422
Size: 695 727
Bme1580I G_kGCm'C
Cuts at: 0 920 1147 1422
Size: 920 227 275
Fragments arranged by size:
920 275 227
BmrI ACTGGGnnnn_n'
Cuts at: 0 935 1422
Size: 935 487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1338 1422
Size: 820 518 84
Fragments arranged by size:
820 518 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1422
Size: 355 1067
BsaI GGTCTCn'nnnn_
Cuts at: 0 1300 1422
Size: 1300 122
BsaHI Gr'CG_yC
Cuts at: 0 345 1129 1422
Size: 345 784 293
Fragments arranged by size:
784 345 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 523 553 1422
Size: 469 30 24 30 869
Fragments arranged by size:
869 469 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 784 825 1422
Size: 544 240 41 597
Fragments arranged by size:
597 544 240 41
BseYI C'CCAG_C
Cuts at: 0 349 552 963 1422
Size: 349 203 411 459
Fragments arranged by size:
459 411 349 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 547 574 1422
Size: 87 460 27 848
Fragments arranged by size:
848 460 87 27
BsiHKAI G_wGCw'C
Cuts at: 0 431 1422
Size: 431 991
Bsp1286I G_dGCh'C
Cuts at: 0 413 431 920 1147 1422
Size: 413 18 489 227 275
Fragments arranged by size:
489 413 275 227 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 85 593 638 1422
Size: 85 508 45 784
Fragments arranged by size:
784 508 85 45
BsrDI GCAATG_nn'
Cuts at: 0 1260 1422
Size: 1260 162
BsrFI r'CCGG_y
Cuts at: 0 80 1422
Size: 80 1342
BstBI TT'CG_AA
Cuts at: 0 470 1422
Size: 470 952
BstEII G'GTnAC_C
Cuts at: 0 1416 1422
Size: 1416 6
BstYI r'GATC_y
Cuts at: 0 533 695 788 1422
Size: 533 162 93 634
Fragments arranged by size:
634 533 162 93
Bsu36I CC'TnA_GG
Cuts at: 0 1369 1422
Size: 1369 53
BtgI C'CryG_G
Cuts at: 0 250 830 1349 1422
Size: 250 580 519 73
Fragments arranged by size:
580 519 250 73
BtsI GCAGTG_nn'
Cuts at: 0 84 204 1363 1422
Size: 84 120 1159 59
Fragments arranged by size:
1159 120 84 59
ClaI AT'CG_AT
Cuts at: 0 365 1422
Size: 365 1057
EaeI y'GGCC_r
Cuts at: 0 185 227 1233 1281 1422
Size: 185 42 1006 48 141
Fragments arranged by size:
1006 185 141 48 42
EarI CTCTTCn'nnn_
Cuts at: 0 1240 1296 1422
Size: 1240 56 126
Fragments arranged by size:
1240 126 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 820 1030 1158 1277 1338 1422
Size: 552 268 210 128 119 61 84
Fragments arranged by size:
552 268 210 128 119 84 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 1137 1422
Size: 907 230 285
Fragments arranged by size:
907 285 230
EcoO109I rG'GnC_Cy
Cuts at: 0 151 627 815 1297 1373 1422
Size: 151 476 188 482 76 49
Fragments arranged by size:
482 476 188 151 76 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1149 1422
Size: 1149 273
HaeII r_GCGC'y
Cuts at: 0 401 777 934 1422
Size: 401 376 157 488
Fragments arranged by size:
488 401 376 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 315 347 499 523 531 555
Size: 199 32 84 32 152 24 8 24
Cuts at: 555 835 867 1381 1413 1422
Size: 280 32 514 32 9
Fragments arranged by size:
514 280 199 152 84 32 32 32
32 24 24 9 8
HincII GTy'rAC
Cuts at: 0 900 1422
Size: 900 522
MfeI C'AATT_G
Cuts at: 0 1148 1422
Size: 1148 274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 521 704 1422
Size: 441 80 183 718
Fragments arranged by size:
718 441 183 80
MscI TGG'CCA
Cuts at: 0 187 1283 1422
Size: 187 1096 139
Fragments arranged by size:
1096 187 139
MslI CAynn'nnrTG
Cuts at: 0 1348 1422
Size: 1348 74
MspA1I CmG'CkG
Cuts at: 0 113 243 552 1092 1422
Size: 113 130 309 540 330
Fragments arranged by size:
540 330 309 130 113
NaeI GCC'GGC
Cuts at: 0 82 1422
Size: 82 1340
NgoMIV G'CCGG_C
Cuts at: 0 80 1422
Size: 80 1342
NspI r_CATG'y
Cuts at: 0 561 1329 1422
Size: 561 768 93
Fragments arranged by size:
768 561 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 1003 1316 1422
Size: 669 334 313 106
Fragments arranged by size:
669 334 313 106
PfoI T'CCnGG_A
Cuts at: 0 797 1422
Size: 797 625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1422
Size: 1050 32 340
Fragments arranged by size:
1050 340 32
PpuMI rG'GwC_Cy
Cuts at: 0 151 627 815 1373 1422
Size: 151 476 188 558 49
Fragments arranged by size:
558 476 188 151 49
PshAI GACnn'nnGTC
Cuts at: 0 279 736 1422
Size: 279 457 686
Fragments arranged by size:
686 457 279
PvuII CAG'CTG
Cuts at: 0 113 552 1092 1422
Size: 113 439 540 330
Fragments arranged by size:
540 439 330 113
SapI GCTCTTCn'nnn_
Cuts at: 0 1240 1422
Size: 1240 182
ScaI AGT'ACT
Cuts at: 0 1288 1422
Size: 1288 134
SexAI A'CCwGG_T
Cuts at: 0 902 1422
Size: 902 520
SmlI C'TyrA_G
Cuts at: 0 334 1137 1422
Size: 334 803 285
Fragments arranged by size:
803 334 285
SphI G_CATG'C
Cuts at: 0 561 1329 1422
Size: 561 768 93
Fragments arranged by size:
768 561 93
StyI C'CwwG_G
Cuts at: 0 1062 1422
Size: 1062 360
TatI w'GTAC_w
Cuts at: 0 482 1286 1422
Size: 482 804 136
Fragments arranged by size:
804 482 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 1137 1169 1422
Size: 469 32 636 32 253
Fragments arranged by size:
636 469 253 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 217 904 1422
Size: 217 687 518
Fragments arranged by size:
687 518 217
XbaI T'CTAG_A
Cuts at: 0 43 1422
Size: 43 1379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 865 1096 1422
Size: 549 316 231 326
Fragments arranged by size:
549 326 316 231
ZraI GAC'GTC
Cuts at: 0 346 1130 1422
Size: 346 784 292
Fragments arranged by size:
784 346 292
Enzymes that do cut:
AatII AccI AcuI AfeI AflII AhdI AleI ApoI
AvaI BanII BbsI BglII Bme1580I BmrI BpmI BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BstBI BstEII BstYI Bsu36I BtgI
BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PflMI PfoI PpiI PpuMI PshAI
PvuII SapI ScaI SexAI SmlI SphI StyI TatI
TstI Tth111I XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflIII AgeI AlfI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstXI BstZ17I BtgZI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII XhoI XmaI XmnI