Restriction map for
pF1KB5815
(Linear) (Six-base) MAPSORT of: KIBB5815.seq Check: 8813 from: 1 to: 1413
KIBB5815.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AleI CACnn'nnGTG
Cuts at: 0 1300 1413
Size: 1300 113
AlwNI CAG_nnn'CTG
Cuts at: 0 355 1332 1413
Size: 355 977 81
Fragments arranged by size:
977 355 81
ApaI G_GGCC'C
Cuts at: 0 851 1413
Size: 851 562
ApoI r'AATT_y
Cuts at: 0 585 1413
Size: 585 828
AvaI C'yCGr_G
Cuts at: 0 164 1413
Size: 164 1249
BanI G'GyrC_C
Cuts at: 0 408 446 1263 1413
Size: 408 38 817 150
Fragments arranged by size:
817 408 150 38
BanII G_rGCy'C
Cuts at: 0 851 893 1084 1413
Size: 851 42 191 329
Fragments arranged by size:
851 329 191 42
BbeI G_GCGC'C
Cuts at: 0 450 1413
Size: 450 963
BglI GCCn_nnn'nGGC
Cuts at: 0 1337 1413
Size: 1337 76
BglII A'GATC_T
Cuts at: 0 1280 1413
Size: 1280 133
BlpI GC'TnA_GC
Cuts at: 0 1030 1413
Size: 1030 383
Bme1580I G_kGCm'C
Cuts at: 0 413 851 1268 1413
Size: 413 438 417 145
Fragments arranged by size:
438 417 413 145
BmgBI CAC'GTC
Cuts at: 0 279 1413
Size: 279 1134
BmrI ACTGGGnnnn_n'
Cuts at: 0 282 547 1413
Size: 282 265 866
Fragments arranged by size:
866 282 265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 741 1314 1413
Size: 624 117 573 99
Fragments arranged by size:
624 573 117 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1413
Size: 838 575
BsaAI yAC'GTr
Cuts at: 0 456 921 1413
Size: 456 465 492
Fragments arranged by size:
492 465 456
BsaHI Gr'CG_yC
Cuts at: 0 447 1048 1256 1413
Size: 447 601 208 157
Fragments arranged by size:
601 447 208 157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 499 1162 1180 1189 1413
Size: 226 273 663 18 9 224
Fragments arranged by size:
663 273 226 224 18 9
BseYI C'CCAG_C
Cuts at: 0 972 1413
Size: 972 441
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1111 1224 1413
Size: 586 525 113 189
Fragments arranged by size:
586 525 189 113
BsiEI CG_ry'CG
Cuts at: 0 135 1413
Size: 135 1278
BsiHKAI G_wGCw'C
Cuts at: 0 218 302 1036 1084 1413
Size: 218 84 734 48 329
Fragments arranged by size:
734 329 218 84 48
BsmI GAATG_Cn'
Cuts at: 0 1026 1413
Size: 1026 387
Bsp1286I G_dGCh'C
Cuts at: 0 218 302 413 851 893 1036 1084 1268
Size: 218 84 111 438 42 143 48 184
Cuts at: 1268 1413
Size: 145
Fragments arranged by size:
438 218 184 145 143 111 84 48
42
BspHI T'CATG_A
Cuts at: 0 1127 1413
Size: 1127 286
BsrBI CCG'CTC
Cuts at: 0 24 51 92 608 1413
Size: 24 27 41 516 805
Fragments arranged by size:
805 516 41 27 24
BsrDI GCAATG_nn'
Cuts at: 0 947 1413
Size: 947 466
BsrFI r'CCGG_y
Cuts at: 0 65 134 405 1413
Size: 65 69 271 1008
Fragments arranged by size:
1008 271 69 65
BssSI C'ACGA_G
Cuts at: 0 244 1413
Size: 244 1169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 544 1359 1413
Size: 544 815 54
Fragments arranged by size:
815 544 54
BstYI r'GATC_y
Cuts at: 0 674 1235 1280 1413
Size: 674 561 45 133
Fragments arranged by size:
674 561 133 45
Bsu36I CC'TnA_GG
Cuts at: 0 1145 1413
Size: 1145 268
BtgI C'CryG_G
Cuts at: 0 413 884 1413
Size: 413 471 529
Fragments arranged by size:
529 471 413
BtsI GCAGTG_nn'
Cuts at: 0 353 1413
Size: 353 1060
DrdI GACnn_nn'nnGTC
Cuts at: 0 931 1413
Size: 931 482
EaeI y'GGCC_r
Cuts at: 0 1101 1413
Size: 1101 312
EarI CTCTTCn'nnn_
Cuts at: 0 365 1413
Size: 365 1048
EcoICRI GAG'CTC
Cuts at: 0 1082 1413
Size: 1082 331
EcoNI CCTnn'n_nnAGG
Cuts at: 0 871 1413
Size: 871 542
EcoO109I rG'GnC_Cy
Cuts at: 0 540 848 1116 1329 1413
Size: 540 308 268 213 84
Fragments arranged by size:
540 308 268 213 84
HaeII r_GCGC'y
Cuts at: 0 161 206 450 1413
Size: 161 45 244 963
Fragments arranged by size:
963 244 161 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1349 1372 1381 1404 1413
Size: 1349 23 9 23 9
Fragments arranged by size:
1349 23 23 9 9
KasI G'GCGC_C
Cuts at: 0 446 1413
Size: 446 967
MslI CAynn'nnrTG
Cuts at: 0 310 1132 1300 1413
Size: 310 822 168 113
Fragments arranged by size:
822 310 168 113
MspA1I CmG'CkG
Cuts at: 0 153 972 1413
Size: 153 819 441
Fragments arranged by size:
819 441 153
NarI GG'CG_CC
Cuts at: 0 447 1413
Size: 447 966
NspI r_CATG'y
Cuts at: 0 714 969 1413
Size: 714 255 444
Fragments arranged by size:
714 444 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 1413
Size: 633 780
PpuMI rG'GwC_Cy
Cuts at: 0 1116 1413
Size: 1116 297
PshAI GACnn'nnGTC
Cuts at: 0 1114 1413
Size: 1114 299
PspOMI G'GGCC_C
Cuts at: 0 847 1413
Size: 847 566
PvuII CAG'CTG
Cuts at: 0 972 1413
Size: 972 441
SacI G_AGCT'C
Cuts at: 0 1084 1413
Size: 1084 329
SapI GCTCTTCn'nnn_
Cuts at: 0 365 1413
Size: 365 1048
SexAI A'CCwGG_T
Cuts at: 0 818 866 1413
Size: 818 48 547
Fragments arranged by size:
818 547 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1337 1413
Size: 1337 76
SfoI GGC'GCC
Cuts at: 0 448 1413
Size: 448 965
SgrAI Cr'CCGG_yG
Cuts at: 0 405 1413
Size: 405 1008
SmaI CCC'GGG
Cuts at: 0 166 1413
Size: 166 1247
SmlI C'TyrA_G
Cuts at: 0 853 1413
Size: 853 560
SphI G_CATG'C
Cuts at: 0 969 1413
Size: 969 444
StuI AGG'CCT
Cuts at: 0 684 859 1413
Size: 684 175 554
Fragments arranged by size:
684 554 175
StyI C'CwwG_G
Cuts at: 0 896 1413
Size: 896 517
TatI w'GTAC_w
Cuts at: 0 317 1413
Size: 317 1096
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 659 691 1413
Size: 659 32 722
Fragments arranged by size:
722 659 32
Tth111I GACn'n_nGTC
Cuts at: 0 820 1255 1413
Size: 820 435 158
Fragments arranged by size:
820 435 158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 703 1413
Size: 703 710
XmaI C'CCGG_G
Cuts at: 0 164 1413
Size: 164 1249
Enzymes that do cut:
AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI EcoNI
EcoO109I HaeII Hin4I KasI MslI MspA1I NarI NspI
PflMI PpuMI PshAI PspOMI PvuII SacI SapI SexAI
SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BmtI BpmI Bpu10I BsaI BsaBI BsaWI
BsaXI BsiWI BsmBI BspEI BspMI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EciI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfcI SgfI SnaBI SpeI SrfI
SspI SwaI TaqII XbaI XhoI XmnI ZraI