(Linear) (Six-base) MAPSORT of: KIBB5817.seq  Check: 3963  from: 1  to: 636



KIBB5817.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     505     636 
   Size:        124     381     131

  Fragments arranged by size: 

                 381     131     124
 
AflIII A'CryG_T

Cuts at:      0     413     636 
   Size:        413     223
 
ApoI r'AATT_y

Cuts at:      0     353     636 
   Size:        353     283
 
BamHI G'GATC_C

Cuts at:      0     522     636 
   Size:        522     114
 
BanII G_rGCy'C

Cuts at:      0     633     636 
   Size:        633       3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329     636 
   Size:        329     307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     619     634     636 
   Size:        283     336      15       2

  Fragments arranged by size: 

                 336     283      15       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     538     636 
   Size:        538      98
 
BsaAI yAC'GTr

Cuts at:      0     416     636 
   Size:        416     220
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286     636 
   Size:        256      30     350

  Fragments arranged by size: 

                 350     256      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     636 
   Size:        400     236
 
BseYI C'CCAG_C

Cuts at:      0     210     636 
   Size:        210     426
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     636 
   Size:        544      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     633     636 
   Size:        633       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     633     636 
   Size:        633       3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     118     636 
   Size:        118     518
 
BsrGI T'GTAC_A

Cuts at:      0     464     636 
   Size:        464     172
 
BstYI r'GATC_y

Cuts at:      0     522     636 
   Size:        522     114
 
BtsI GCAGTG_nn'

Cuts at:      0     137     636 
   Size:        137     499
 
EaeI y'GGCC_r

Cuts at:      0     497     636 
   Size:        497     139
 
EcoICRI GAG'CTC

Cuts at:      0     631     636 
   Size:        631       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     283     505     619     634     636 
   Size:        124     159     222     114      15       2

  Fragments arranged by size: 

                 222     159     124     114      15       2
 
HincII GTy'rAC

Cuts at:      0     305     636 
   Size:        305     331
 
MscI TGG'CCA

Cuts at:      0     499     636 
   Size:        499     137
 
MslI CAynn'nnrTG

Cuts at:      0     434     636 
   Size:        434     202
 
NspI r_CATG'y

Cuts at:      0     609     636 
   Size:        609      27
 
PmlI CAC'GTG

Cuts at:      0     416     636 
   Size:        416     220
 
PstI C_TGCA'G

Cuts at:      0     287     636 
   Size:        287     349
 
SacI G_AGCT'C

Cuts at:      0     633     636 
   Size:        633       3
 
SfcI C'TryA_G

Cuts at:      0     283     636 
   Size:        283     353
 
SphI G_CATG'C

Cuts at:      0     609     636 
   Size:        609      27
 
SspI AAT'ATT

Cuts at:      0     150     636 
   Size:        150     486
 
StyI C'CwwG_G

Cuts at:      0     293     347     500     636 
   Size:        293      54     153     136

  Fragments arranged by size: 

                 293     153     136      54
 
TatI w'GTAC_w

Cuts at:      0     464     636 
   Size:        464     172
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     256     288     507     539     636 
   Size:        256      32     219      32      97

  Fragments arranged by size: 

                 256     219      97      32      32
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BamHI    BanII     BbsI     BpmI   Bpu10I
    BsaAI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrGI    BstYI     BtsI     EaeI  EcoICRI  Eco57MI   HincII     MscI
     MslI     NspI     PmlI     PstI     SacI     SfcI     SphI     SspI
     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI