Restriction map for
pF1KB5826
(Linear) (Six-base) MAPSORT of: KIBB5826.seq Check: 952 from: 1 to: 1425
KIBB5826.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 853 1183 1320 1425
Size: 796 57 330 137 105
Fragments arranged by size:
796 330 137 105 57
AfeI AGC'GCT
Cuts at: 0 169 1425
Size: 169 1256
AflIII A'CryG_T
Cuts at: 0 850 1425
Size: 850 575
AlwNI CAG_nnn'CTG
Cuts at: 0 104 149 597 1425
Size: 104 45 448 828
Fragments arranged by size:
828 448 104 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 385 418 1425
Size: 385 33 1007
Fragments arranged by size:
1007 385 33
BamHI G'GATC_C
Cuts at: 0 873 1411 1425
Size: 873 538 14
Fragments arranged by size:
873 538 14
BanI G'GyrC_C
Cuts at: 0 704 997 1171 1203 1425
Size: 704 293 174 32 222
Fragments arranged by size:
704 293 222 174 32
BanII G_rGCy'C
Cuts at: 0 683 1425
Size: 683 742
BbvCI CC'TCA_GC
Cuts at: 0 844 1425
Size: 844 581
BfrBI ATG'CAT
Cuts at: 0 116 1425
Size: 116 1309
BglI GCCn_nnn'nGGC
Cuts at: 0 652 1425
Size: 652 773
BlpI GC'TnA_GC
Cuts at: 0 714 1425
Size: 714 711
Bme1580I G_kGCm'C
Cuts at: 0 259 345 1002 1103 1425
Size: 259 86 657 101 322
Fragments arranged by size:
657 322 259 101 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 1038 1425
Size: 339 699 387
Fragments arranged by size:
699 387 339
BmtI G_CTAG'C
Cuts at: 0 208 1425
Size: 208 1217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 702 1425
Size: 702 723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 619 782 1425
Size: 400 219 163 643
Fragments arranged by size:
643 400 219 163
Bpu10I CC'TnA_GC
Cuts at: 0 844 1425
Size: 844 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 403 1425
Size: 163 240 1022
Fragments arranged by size:
1022 240 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 1257 1425
Size: 1257 168
BsaBI GATnn'nnATC
Cuts at: 0 980 1425
Size: 980 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 1425
Size: 1264 30 131
Fragments arranged by size:
1264 131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 400 1425
Size: 49 351 1025
Fragments arranged by size:
1025 351 49
BseYI C'CCAG_C
Cuts at: 0 332 656 941 1001 1425
Size: 332 324 285 60 424
Fragments arranged by size:
424 332 324 285 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 377 568 875 1425
Size: 296 81 191 307 550
Fragments arranged by size:
550 307 296 191 81
BsiHKAI G_wGCw'C
Cuts at: 0 683 1425
Size: 683 742
BsmI GAATG_Cn'
Cuts at: 0 118 1425
Size: 118 1307
Bsp1286I G_dGCh'C
Cuts at: 0 259 345 683 1002 1103 1425
Size: 259 86 338 319 101 322
Fragments arranged by size:
338 322 319 259 101 86
BsrFI r'CCGG_y
Cuts at: 0 161 1425
Size: 161 1264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1107 1425
Size: 1107 318
BstYI r'GATC_y
Cuts at: 0 873 1411 1425
Size: 873 538 14
Fragments arranged by size:
873 538 14
BtgI C'CryG_G
Cuts at: 0 371 892 1275 1425
Size: 371 521 383 150
Fragments arranged by size:
521 383 371 150
BtsI GCAGTG_nn'
Cuts at: 0 284 1425
Size: 284 1141
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1425
Size: 1069 356
EaeI y'GGCC_r
Cuts at: 0 93 1425
Size: 93 1332
EarI CTCTTCn'nnn_
Cuts at: 0 959 1425
Size: 959 466
EcoICRI GAG'CTC
Cuts at: 0 681 1425
Size: 681 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 619 782 796 853 1183 1320 1425
Size: 400 219 163 14 57 330 137 105
Fragments arranged by size:
400 330 219 163 137 105 57 14
EcoO109I rG'GnC_Cy
Cuts at: 0 324 669 780 1062 1318 1425
Size: 324 345 111 282 256 107
Fragments arranged by size:
345 324 282 256 111 107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 946 1119 1143 1425
Size: 946 173 24 282
Fragments arranged by size:
946 282 173 24
FspI TGC'GCA
Cuts at: 0 1090 1425
Size: 1090 335
HaeII r_GCGC'y
Cuts at: 0 171 1425
Size: 171 1254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 1264 1296 1425
Size: 778 32 454 32 129
Fragments arranged by size:
778 454 129 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1425
Size: 446 979
MscI TGG'CCA
Cuts at: 0 95 1425
Size: 95 1330
MspA1I CmG'CkG
Cuts at: 0 588 621 1425
Size: 588 33 804
Fragments arranged by size:
804 588 33
NaeI GCC'GGC
Cuts at: 0 163 1425
Size: 163 1262
NcoI C'CATG_G
Cuts at: 0 371 1275 1425
Size: 371 904 150
Fragments arranged by size:
904 371 150
NgoMIV G'CCGG_C
Cuts at: 0 161 1425
Size: 161 1264
NheI G'CTAG_C
Cuts at: 0 204 1425
Size: 204 1221
NsiI A_TGCA'T
Cuts at: 0 118 1425
Size: 118 1307
NspI r_CATG'y
Cuts at: 0 47 854 1425
Size: 47 807 571
Fragments arranged by size:
807 571 47
PciI A'CATG_T
Cuts at: 0 850 1425
Size: 850 575
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 893 1425
Size: 372 521 532
Fragments arranged by size:
532 521 372
PfoI T'CCnGG_A
Cuts at: 0 749 766 1229 1425
Size: 749 17 463 196
Fragments arranged by size:
749 463 196 17
PpuMI rG'GwC_Cy
Cuts at: 0 780 1062 1318 1425
Size: 780 282 256 107
Fragments arranged by size:
780 282 256 107
PstI C_TGCA'G
Cuts at: 0 593 734 1078 1425
Size: 593 141 344 347
Fragments arranged by size:
593 347 344 141
PvuII CAG'CTG
Cuts at: 0 588 621 1425
Size: 588 33 804
Fragments arranged by size:
804 588 33
SacI G_AGCT'C
Cuts at: 0 683 1425
Size: 683 742
SfcI C'TryA_G
Cuts at: 0 589 673 730 1074 1425
Size: 589 84 57 344 351
Fragments arranged by size:
589 351 344 84 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 652 1425
Size: 652 773
SmlI C'TyrA_G
Cuts at: 0 178 382 1425
Size: 178 204 1043
Fragments arranged by size:
1043 204 178
SspI AAT'ATT
Cuts at: 0 1398 1425
Size: 1398 27
StuI AGG'CCT
Cuts at: 0 1057 1425
Size: 1057 368
StyI C'CwwG_G
Cuts at: 0 371 1275 1425
Size: 371 904 150
Fragments arranged by size:
904 371 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 955 985 1057 1425
Size: 955 30 72 368
Fragments arranged by size:
955 368 72 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 345 377 858 873 890 905 1425
Size: 345 32 481 15 17 15 520
Fragments arranged by size:
520 481 345 32 17 15 15
Enzymes that do cut:
AcuI AfeI AflIII AlwNI BaeI BamHI BanI BanII
BbvCI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BsrFI BstAPI BstYI BtgI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
FspI HaeII Hin4I MmeI MscI MspA1I NaeI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PpuMI
PstI PvuII SacI SfcI SfiI SmlI SspI StuI
StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BbeI BbsI BcgI BciVI BclI
BglII BmgBI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoNI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NarI NdeI NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI