Restriction map for
pF1KB5827
(Linear) (Six-base) MAPSORT of: KIBB5827.seq Check: 3599 from: 1 to: 2067
KIBB5827.orf
With 182 enzymes: SgfI *
September 25, 2012 15:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1667 2067
Size: 1667 400
AhdI GACnn_n'nnGTC
Cuts at: 0 1503 2067
Size: 1503 564
AleI CACnn'nnGTG
Cuts at: 0 1669 2067
Size: 1669 398
AlwNI CAG_nnn'CTG
Cuts at: 0 1205 1271 1347 2067
Size: 1205 66 76 720
Fragments arranged by size:
1205 720 76 66
ApaI G_GGCC'C
Cuts at: 0 783 1917 2067
Size: 783 1134 150
Fragments arranged by size:
1134 783 150
AvaI C'yCGr_G
Cuts at: 0 112 2067
Size: 112 1955
AvrII C'CTAG_G
Cuts at: 0 711 2067
Size: 711 1356
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1285 1318 2067
Size: 1285 33 749
Fragments arranged by size:
1285 749 33
BanI G'GyrC_C
Cuts at: 0 67 73 1886 2067
Size: 67 6 1813 181
Fragments arranged by size:
1813 181 67 6
BanII G_rGCy'C
Cuts at: 0 584 783 1154 1654 1701 1917 2067
Size: 584 199 371 500 47 216 150
Fragments arranged by size:
584 500 371 216 199 150 47
BbeI G_GCGC'C
Cuts at: 0 71 77 2067
Size: 71 6 1990
Fragments arranged by size:
1990 71 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1464 1975 2067
Size: 1464 511 92
Fragments arranged by size:
1464 511 92
BbvCI CC'TCA_GC
Cuts at: 0 733 1351 2067
Size: 733 618 716
Fragments arranged by size:
733 716 618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 2067
Size: 93 34 1940
Fragments arranged by size:
1940 93 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 676 2067
Size: 676 1391
BfrBI ATG'CAT
Cuts at: 0 912 2067
Size: 912 1155
BglI GCCn_nnn'nGGC
Cuts at: 0 89 1884 2067
Size: 89 1795 183
Fragments arranged by size:
1795 183 89
BlpI GC'TnA_GC
Cuts at: 0 484 1264 2067
Size: 484 780 803
Fragments arranged by size:
803 780 484
Bme1580I G_kGCm'C
Cuts at: 0 783 1917 2046 2067
Size: 783 1134 129 21
Fragments arranged by size:
1134 783 129 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 2049 2067
Size: 2049 18
BmtI G_CTAG'C
Cuts at: 0 1574 2067
Size: 1574 493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 598 1227 1273 2067
Size: 352 246 629 46 794
Fragments arranged by size:
794 629 352 246 46
Bpu10I CC'TnA_GC
Cuts at: 0 733 1156 1223 1351 2067
Size: 733 423 67 128 716
Fragments arranged by size:
733 716 423 128 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1918 2067
Size: 1312 606 149
Fragments arranged by size:
1312 606 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 578 2067
Size: 578 1489
BsaAI yAC'GTr
Cuts at: 0 535 2067
Size: 535 1532
BsaHI Gr'CG_yC
Cuts at: 0 68 74 2067
Size: 68 6 1993
Fragments arranged by size:
1993 68 6
BsaWI w'CCGG_w
Cuts at: 0 1502 2067
Size: 1502 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1009 1039 2067
Size: 1009 30 1028
Fragments arranged by size:
1028 1009 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 101 281 455 1444 2067
Size: 101 180 174 989 623
Fragments arranged by size:
989 623 180 174 101
BseYI C'CCAG_C
Cuts at: 0 831 852 1006 1626 1678 2060 2067
Size: 831 21 154 620 52 382 7
Fragments arranged by size:
831 620 382 154 52 21 7
BsiEI CG_ry'CG
Cuts at: 0 84 2067
Size: 84 1983
BsiHKAI G_wGCw'C
Cuts at: 0 584 1154 1259 1925 2067
Size: 584 570 105 666 142
Fragments arranged by size:
666 584 570 142 105
Bsp1286I G_dGCh'C
Cuts at: 0 584 783 1154 1259 1654 1701 1917 1925
Size: 584 199 371 105 395 47 216 8
Cuts at: 1925 2046 2067
Size: 121 21
Fragments arranged by size:
584 395 371 216 199 121 105 47
21 8
BspHI T'CATG_A
Cuts at: 0 200 498 2067
Size: 200 298 1569
Fragments arranged by size:
1569 298 200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 213 374 784 1410 1575 1667 2067
Size: 213 161 410 626 165 92 400
Fragments arranged by size:
626 410 400 213 165 161 92
BsrBI CCG'CTC
Cuts at: 0 183 1644 2067
Size: 183 1461 423
Fragments arranged by size:
1461 423 183
BsrFI r'CCGG_y
Cuts at: 0 70 76 1538 2067
Size: 70 6 1462 529
Fragments arranged by size:
1462 529 70 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 1893 2067
Size: 799 1094 174
Fragments arranged by size:
1094 799 174
BstEII G'GTnAC_C
Cuts at: 0 652 1950 2067
Size: 652 1298 117
Fragments arranged by size:
1298 652 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 419 2067
Size: 419 1648
BstYI r'GATC_y
Cuts at: 0 1796 2067
Size: 1796 271
BtgI C'CryG_G
Cuts at: 0 701 1667 2067
Size: 701 966 400
Fragments arranged by size:
966 701 400
BtsI GCAGTG_nn'
Cuts at: 0 1636 1923 2067
Size: 1636 287 144
Fragments arranged by size:
1636 287 144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 263 298 2067
Size: 263 35 1769
Fragments arranged by size:
1769 263 35
EaeI y'GGCC_r
Cuts at: 0 81 434 1536 2067
Size: 81 353 1102 531
Fragments arranged by size:
1102 531 353 81
EagI C'GGCC_G
Cuts at: 0 81 2067
Size: 81 1986
EcoICRI GAG'CTC
Cuts at: 0 582 1152 2067
Size: 582 570 915
Fragments arranged by size:
915 582 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 598 1227 1273 2067
Size: 352 246 629 46 794
Fragments arranged by size:
794 629 352 246 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 811 925 2067
Size: 370 441 114 1142
Fragments arranged by size:
1142 441 370 114
EcoO109I rG'GnC_Cy
Cuts at: 0 225 429 737 780 1407 1429 1902 2067
Size: 225 204 308 43 627 22 473 165
Fragments arranged by size:
627 473 308 225 204 165 43 22
EcoRV GAT'ATC
Cuts at: 0 2057 2067
Size: 2057 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 975 2067
Size: 975 1092
HaeII r_GCGC'y
Cuts at: 0 71 77 112 2067
Size: 71 6 35 1955
Fragments arranged by size:
1955 71 35 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 2067
Size: 1489 32 546
Fragments arranged by size:
1489 546 32
HindIII A'AGCT_T
Cuts at: 0 1330 2067
Size: 1330 737
KasI G'GCGC_C
Cuts at: 0 67 73 2067
Size: 67 6 1994
Fragments arranged by size:
1994 67 6
MscI TGG'CCA
Cuts at: 0 436 2067
Size: 436 1631
MslI CAynn'nnrTG
Cuts at: 0 417 497 772 1669 1706 1865 1928 2023
Size: 417 80 275 897 37 159 63 95
Cuts at: 2023 2067
Size: 44
Fragments arranged by size:
897 417 275 159 95 80 63 44
37
MspA1I CmG'CkG
Cuts at: 0 1023 1215 1622 1689 2067
Size: 1023 192 407 67 378
Fragments arranged by size:
1023 407 378 192 67
NaeI GCC'GGC
Cuts at: 0 72 78 2067
Size: 72 6 1989
Fragments arranged by size:
1989 72 6
NarI GG'CG_CC
Cuts at: 0 68 74 2067
Size: 68 6 1993
Fragments arranged by size:
1993 68 6
NdeI CA'TA_TG
Cuts at: 0 418 2067
Size: 418 1649
NgoMIV G'CCGG_C
Cuts at: 0 70 76 2067
Size: 70 6 1991
Fragments arranged by size:
1991 70 6
NheI G'CTAG_C
Cuts at: 0 1570 2067
Size: 1570 497
NsiI A_TGCA'T
Cuts at: 0 914 2067
Size: 914 1153
NspI r_CATG'y
Cuts at: 0 1940 2067
Size: 1940 127
PasI CC'CwG_GG
Cuts at: 0 783 2067
Size: 783 1284
PflMI CCAn_nnn'nTGG
Cuts at: 0 776 1205 2067
Size: 776 429 862
Fragments arranged by size:
862 776 429
PfoI T'CCnGG_A
Cuts at: 0 722 2067
Size: 722 1345
PpuMI rG'GwC_Cy
Cuts at: 0 225 429 737 1429 2067
Size: 225 204 308 692 638
Fragments arranged by size:
692 638 308 225 204
PshAI GACnn'nnGTC
Cuts at: 0 596 2067
Size: 596 1471
PspOMI G'GGCC_C
Cuts at: 0 779 1913 2067
Size: 779 1134 154
Fragments arranged by size:
1134 779 154
PstI C_TGCA'G
Cuts at: 0 371 1016 2067
Size: 371 645 1051
Fragments arranged by size:
1051 645 371
PvuII CAG'CTG
Cuts at: 0 1023 1215 1622 1689 2067
Size: 1023 192 407 67 378
Fragments arranged by size:
1023 407 378 192 67
SacI G_AGCT'C
Cuts at: 0 584 1154 2067
Size: 584 570 913
Fragments arranged by size:
913 584 570
SbfI CC_TGCA'GG
Cuts at: 0 1016 2067
Size: 1016 1051
SfcI C'TryA_G
Cuts at: 0 367 510 1012 1478 1602 1617 1792 2011
Size: 367 143 502 466 124 15 175 219
Cuts at: 2011 2067
Size: 56
Fragments arranged by size:
502 466 367 219 175 143 124 56
15
SfoI GGC'GCC
Cuts at: 0 69 75 2067
Size: 69 6 1992
Fragments arranged by size:
1992 69 6
SgrAI Cr'CCGG_yG
Cuts at: 0 70 76 2067
Size: 70 6 1991
Fragments arranged by size:
1991 70 6
SmlI C'TyrA_G
Cuts at: 0 1327 1897 2067
Size: 1327 570 170
Fragments arranged by size:
1327 570 170
SphI G_CATG'C
Cuts at: 0 1940 2067
Size: 1940 127
StuI AGG'CCT
Cuts at: 0 1525 2067
Size: 1525 542
StyI C'CwwG_G
Cuts at: 0 711 926 950 2067
Size: 711 215 24 1117
Fragments arranged by size:
1117 711 215 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1020 2067
Size: 1020 1047
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1009 1041 2067
Size: 809 32 168 32 1026
Fragments arranged by size:
1026 809 168 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 1248 1686 2067
Size: 888 360 438 381
Fragments arranged by size:
888 438 381 360
XmnI GAAnn'nnTTC
Cuts at: 0 1316 2067
Size: 1316 751
Enzymes that do cut:
AarI AhdI AleI AlwNI ApaI AvaI AvrII BaeI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII
BstXI BstYI BtgI BtsI CspCI EaeI EagI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII
KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NsiI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SbfI SfcI SfoI SgrAI
SmlI SphI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BamHI BclI BglII BmgBI BplI BsaBI BsgI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EarI
EciI EcoRI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI NcoI NotI NruI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI