Restriction map for
pF1KB5828
(Linear) (Six-base) MAPSORT of: KIBB5828.seq Check: 6023 from: 1 to: 1440
KIBB5828.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1102 1440
Size: 1102 338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1440
Size: 313 1127
AfeI AGC'GCT
Cuts at: 0 611 1440
Size: 611 829
AleI CACnn'nnGTG
Cuts at: 0 175 1440
Size: 175 1265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 901 1440
Size: 901 539
AlwNI CAG_nnn'CTG
Cuts at: 0 33 1440
Size: 33 1407
ApaI G_GGCC'C
Cuts at: 0 1037 1440
Size: 1037 403
ApaLI G'TGCA_C
Cuts at: 0 310 1440
Size: 310 1130
AscI GG'CGCG_CC
Cuts at: 0 215 1440
Size: 215 1225
AvaI C'yCGr_G
Cuts at: 0 72 382 785 1257 1440
Size: 72 310 403 472 183
Fragments arranged by size:
472 403 310 183 72
BamHI G'GATC_C
Cuts at: 0 1415 1440
Size: 1415 25
BanI G'GyrC_C
Cuts at: 0 52 339 548 1047 1440
Size: 52 287 209 499 393
Fragments arranged by size:
499 393 287 209 52
BanII G_rGCy'C
Cuts at: 0 280 895 976 1037 1150 1440
Size: 280 615 81 61 113 290
Fragments arranged by size:
615 290 280 113 81 61
BbeI G_GCGC'C
Cuts at: 0 56 343 552 1051 1440
Size: 56 287 209 499 389
Fragments arranged by size:
499 389 287 209 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 189 1440
Size: 155 34 1251
Fragments arranged by size:
1251 155 34
BglI GCCn_nnn'nGGC
Cuts at: 0 97 337 1022 1103 1261 1440
Size: 97 240 685 81 158 179
Fragments arranged by size:
685 240 179 158 97 81
BglII A'GATC_T
Cuts at: 0 944 1440
Size: 944 496
BlpI GC'TnA_GC
Cuts at: 0 889 1440
Size: 889 551
Bme1580I G_kGCm'C
Cuts at: 0 314 1037 1266 1440
Size: 314 723 229 174
Fragments arranged by size:
723 314 229 174
Bpu10I CC'TnA_GC
Cuts at: 0 34 1440
Size: 34 1406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 538 808 1440
Size: 409 129 270 632
Fragments arranged by size:
632 409 270 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 1310 1440
Size: 525 785 130
Fragments arranged by size:
785 525 130
BsaHI Gr'CG_yC
Cuts at: 0 53 340 549 1048 1440
Size: 53 287 209 499 392
Fragments arranged by size:
499 392 287 209 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1021 1051 1150 1180 1440
Size: 1021 30 99 30 260
Fragments arranged by size:
1021 260 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 1440
Size: 676 764
BseYI C'CCAG_C
Cuts at: 0 15 141 180 1440
Size: 15 126 39 1260
Fragments arranged by size:
1260 126 39 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1440
Size: 1205 235
BsiEI CG_ry'CG
Cuts at: 0 92 127 1247 1440
Size: 92 35 1120 193
Fragments arranged by size:
1120 193 92 35
BsiHKAI G_wGCw'C
Cuts at: 0 314 415 446 707 1440
Size: 314 101 31 261 733
Fragments arranged by size:
733 314 261 101 31
BsiWI C'GTAC_G
Cuts at: 0 206 1440
Size: 206 1234
BsmI GAATG_Cn'
Cuts at: 0 1132 1440
Size: 1132 308
Bsp1286I G_dGCh'C
Cuts at: 0 280 314 415 446 707 895 976 1037
Size: 280 34 101 31 261 188 81 61
Cuts at: 1037 1150 1266 1440
Size: 113 116 174
Fragments arranged by size:
280 261 188 174 116 113 101 81
61 34 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 1102 1440
Size: 507 595 338
Fragments arranged by size:
595 507 338
BsrFI r'CCGG_y
Cuts at: 0 342 545 1242 1341 1371 1440
Size: 342 203 697 99 30 69
Fragments arranged by size:
697 342 203 99 69 30
BsrGI T'GTAC_A
Cuts at: 0 1088 1440
Size: 1088 352
BssHII G'CGCG_C
Cuts at: 0 215 541 583 1025 1440
Size: 215 326 42 442 415
Fragments arranged by size:
442 415 326 215 42
BssSI C'ACGA_G
Cuts at: 0 969 1440
Size: 969 471
BstXI CCAn_nnnn'nTGG
Cuts at: 0 604 1440
Size: 604 836
BstYI r'GATC_y
Cuts at: 0 252 944 1415 1440
Size: 252 692 471 25
Fragments arranged by size:
692 471 252 25
BtgI C'CryG_G
Cuts at: 0 41 86 134 248 1010 1080 1203 1411
Size: 41 45 48 114 762 70 123 208
Cuts at: 1411 1440
Size: 29
Fragments arranged by size:
762 208 123 114 70 48 45 41
29
EaeI y'GGCC_r
Cuts at: 0 83 89 360 488 1440
Size: 83 6 271 128 952
Fragments arranged by size:
952 271 128 83 6
EagI C'GGCC_G
Cuts at: 0 89 1440
Size: 89 1351
EarI CTCTTCn'nnn_
Cuts at: 0 268 619 1440
Size: 268 351 821
Fragments arranged by size:
821 351 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1029 1425 1440
Size: 973 56 396 15
Fragments arranged by size:
973 396 56 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1440
Size: 313 1127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 556 853 1141 1440
Size: 514 42 297 288 299
Fragments arranged by size:
514 299 297 288 42
EcoO109I rG'GnC_Cy
Cuts at: 0 378 861 1034 1440
Size: 378 483 173 406
Fragments arranged by size:
483 406 378 173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1380 1440
Size: 1380 60
HaeII r_GCGC'y
Cuts at: 0 41 56 72 233 343 552 613 902
Size: 41 15 16 161 110 209 61 289
Cuts at: 902 1051 1187 1440
Size: 149 136 253
Fragments arranged by size:
289 253 209 161 149 136 110 61
41 16 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1267 1299 1307 1339 1440
Size: 781 32 454 32 8 32 101
Fragments arranged by size:
781 454 101 32 32 32 8
KasI G'GCGC_C
Cuts at: 0 52 339 548 1047 1440
Size: 52 287 209 499 393
Fragments arranged by size:
499 393 287 209 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1440
Size: 1139 301
MscI TGG'CCA
Cuts at: 0 490 1440
Size: 490 950
MslI CAynn'nnrTG
Cuts at: 0 175 502 1440
Size: 175 327 938
Fragments arranged by size:
938 327 175
MspA1I CmG'CkG
Cuts at: 0 15 43 88 94 136 250 804 907
Size: 15 28 45 6 42 114 554 103
Cuts at: 907 1012 1053 1413 1440
Size: 105 41 360 27
Fragments arranged by size:
554 360 114 105 103 45 42 41
28 27 15 6
NaeI GCC'GGC
Cuts at: 0 344 547 1343 1440
Size: 344 203 796 97
Fragments arranged by size:
796 344 203 97
NarI GG'CG_CC
Cuts at: 0 53 340 549 1048 1440
Size: 53 287 209 499 392
Fragments arranged by size:
499 392 287 209 53
NcoI C'CATG_G
Cuts at: 0 1203 1440
Size: 1203 237
NgoMIV G'CCGG_C
Cuts at: 0 342 545 1341 1440
Size: 342 203 796 99
Fragments arranged by size:
796 342 203 99
NotI GC'GGCC_GC
Cuts at: 0 89 1440
Size: 89 1351
NspI r_CATG'y
Cuts at: 0 189 1164 1440
Size: 189 975 276
Fragments arranged by size:
975 276 189
PasI CC'CwG_GG
Cuts at: 0 304 1440
Size: 304 1136
PflMI CCAn_nnn'nTGG
Cuts at: 0 947 1440
Size: 947 493
PpuMI rG'GwC_Cy
Cuts at: 0 378 1440
Size: 378 1062
PspOMI G'GGCC_C
Cuts at: 0 1033 1440
Size: 1033 407
PstI C_TGCA'G
Cuts at: 0 168 906 920 1440
Size: 168 738 14 520
Fragments arranged by size:
738 520 168 14
PvuII CAG'CTG
Cuts at: 0 15 1440
Size: 15 1425
RsrII CG'GwC_CG
Cuts at: 0 1287 1440
Size: 1287 153
SacII CC_GC'GG
Cuts at: 0 44 89 137 251 1013 1414 1440
Size: 44 45 48 114 762 401 26
Fragments arranged by size:
762 401 114 48 45 44 26
SanDI GG'GwC_CC
Cuts at: 0 378 1440
Size: 378 1062
SapI GCTCTTCn'nnn_
Cuts at: 0 619 1440
Size: 619 821
SbfI CC_TGCA'GG
Cuts at: 0 920 1440
Size: 920 520
SexAI A'CCwGG_T
Cuts at: 0 526 1440
Size: 526 914
SfcI C'TryA_G
Cuts at: 0 164 225 902 916 1440
Size: 164 61 677 14 524
Fragments arranged by size:
677 524 164 61 14
SfoI GGC'GCC
Cuts at: 0 54 341 550 1049 1440
Size: 54 287 209 499 391
Fragments arranged by size:
499 391 287 209 54
SgrAI Cr'CCGG_yG
Cuts at: 0 545 1440
Size: 545 895
SmaI CCC'GGG
Cuts at: 0 74 384 787 1259 1440
Size: 74 310 403 472 181
Fragments arranged by size:
472 403 310 181 74
SmlI C'TyrA_G
Cuts at: 0 424 553 823 1440
Size: 424 129 270 617
Fragments arranged by size:
617 424 270 129
StuI AGG'CCT
Cuts at: 0 430 1282 1440
Size: 430 852 158
Fragments arranged by size:
852 430 158
StyI C'CwwG_G
Cuts at: 0 467 792 842 989 1203 1440
Size: 467 325 50 147 214 237
Fragments arranged by size:
467 325 237 214 147 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1275 1440
Size: 1275 165
TatI w'GTAC_w
Cuts at: 0 1088 1440
Size: 1088 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1440
Size: 1021 32 387
Fragments arranged by size:
1021 387 32
Tth111I GACn'n_nGTC
Cuts at: 0 1321 1440
Size: 1321 119
XmaI C'CCGG_G
Cuts at: 0 72 382 785 1257 1440
Size: 72 310 403 472 183
Fragments arranged by size:
472 403 310 183 72
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AscI AvaI BamHI BanI BanII BbeI BcgI BglI
BglII BlpI Bme1580I Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspMI BsrFI BsrGI BssHII BssSI BstXI BstYI BtgI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PflMI PpuMI
PspOMI PstI PvuII RsrII SacII SanDI SapI SbfI
SexAI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AseI AsiSI AvrII BaeI BbsI BbvCI
BciVI BclI BfrBI BmgBI BmrI BmtI BplI BpmI
BsaAI BsaBI BsaWI BsmBI BspEI BspHI BsrBI BsrDI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI
SalI ScaI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XhoI XmnI ZraI