(Linear) (Six-base) MAPSORT of: KIBB5829.seq  Check: 1649  from: 1  to: 372



KIBB5829.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     372 
   Size:          9     363
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     235     372 
   Size:         69     166     137

  Fragments arranged by size: 

                 166     137      69
 
BamHI G'GATC_C

Cuts at:      0     242     372 
   Size:        242     130
 
BanI G'GyrC_C

Cuts at:      0     163     182     372 
   Size:        163      19     190

  Fragments arranged by size: 

                 190     163      19
 
BanII G_rGCy'C

Cuts at:      0     120     335     372 
   Size:        120     215      37

  Fragments arranged by size: 

                 215     120      37
 
BbvCI CC'TCA_GC

Cuts at:      0     228     372 
   Size:        228     144
 
BclI T'GATC_A

Cuts at:      0     342     372 
   Size:        342      30
 
BglII A'GATC_T

Cuts at:      0      28     372 
   Size:         28     344
 
BlpI GC'TnA_GC

Cuts at:      0     334     372 
   Size:        334      38
 
Bme1580I G_kGCm'C

Cuts at:      0     168     372 
   Size:        168     204
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     325     372 
   Size:        325      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     308     372 
   Size:        255      53      64

  Fragments arranged by size: 

                 255      64      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     228     372 
   Size:        228     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     372 
   Size:         20     352
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      74     271     372 
   Size:         74     197     101

  Fragments arranged by size: 

                 197     101      74
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     281     311     372 
   Size:        281      30      61

  Fragments arranged by size: 

                 281      61      30
 
BseYI C'CCAG_C

Cuts at:      0     137     372 
   Size:        137     235
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     372 
   Size:        335      37
 
Bsp1286I G_dGCh'C

Cuts at:      0     120     168     335     372 
   Size:        120      48     167      37

  Fragments arranged by size: 

                 167     120      48      37
 
BstYI r'GATC_y

Cuts at:      0       4      28     147     217     242     372 
   Size:          4      24     119      70      25     130

  Fragments arranged by size: 

                 130     119      70      25      24       4
 
BtsI GCAGTG_nn'

Cuts at:      0      54     372 
   Size:         54     318
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     282     317     372 
   Size:        282      35      55

  Fragments arranged by size: 

                 282      55      35
 
EcoICRI GAG'CTC

Cuts at:      0     333     372 
   Size:        333      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     255     308     372 
   Size:          9     246      53      64

  Fragments arranged by size: 

                 246      64      53       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     372 
   Size:         66     306
 
HindIII A'AGCT_T

Cuts at:      0      38     372 
   Size:         38     334
 
PfoI T'CCnGG_A

Cuts at:      0     285     372 
   Size:        285      87
 
PstI C_TGCA'G

Cuts at:      0      49     372 
   Size:         49     323
 
SacI G_AGCT'C

Cuts at:      0     335     372 
   Size:        335      37
 
SfcI C'TryA_G

Cuts at:      0      45     372 
   Size:         45     327
 
SmlI C'TyrA_G

Cuts at:      0      35     372 
   Size:         35     337
 
StuI AGG'CCT

Cuts at:      0     254     276     372 
   Size:        254      22      96

  Fragments arranged by size: 

                 254      96      22
 
StyI C'CwwG_G

Cuts at:      0     255     372 
   Size:        255     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     151     372 
   Size:        151     221
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     281     313     372 
   Size:        281      32      59

  Fragments arranged by size: 

                 281      59      32
 

 Enzymes that do cut:

     AcuI    AlwNI    BamHI     BanI    BanII    BbvCI     BclI    BglII
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI
    BseYI  BsiHKAI Bsp1286I    BstYI     BtsI    CspCI  EcoICRI  Eco57MI
 EcoO109I  HindIII     PfoI     PstI     SacI     SfcI     SmlI     StuI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI    BmgBI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI