Restriction map for
pF1KB5835
(Linear) (Six-base) MAPSORT of: KIBB5835.seq Check: 5146 from: 1 to: 1590
KIBB5835.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1544 1590
Size: 610 934 46
Fragments arranged by size:
934 610 46
AflII C'TTAA_G
Cuts at: 0 1267 1590
Size: 1267 323
AflIII A'CryG_T
Cuts at: 0 384 1590
Size: 384 1206
AhdI GACnn_n'nnGTC
Cuts at: 0 564 1590
Size: 564 1026
ApaI G_GGCC'C
Cuts at: 0 32 172 1117 1590
Size: 32 140 945 473
Fragments arranged by size:
945 473 140 32
ApaLI G'TGCA_C
Cuts at: 0 79 1590
Size: 79 1511
ApoI r'AATT_y
Cuts at: 0 1133 1590
Size: 1133 457
AvaI C'yCGr_G
Cuts at: 0 1460 1590
Size: 1460 130
BamHI G'GATC_C
Cuts at: 0 1575 1590
Size: 1575 15
BanII G_rGCy'C
Cuts at: 0 32 172 359 953 1117 1310 1413 1461
Size: 32 140 187 594 164 193 103 48
Cuts at: 1461 1590
Size: 129
Fragments arranged by size:
594 193 187 164 140 129 103 48
32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 785 819 1590
Size: 785 34 771
Fragments arranged by size:
785 771 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 573 1590
Size: 573 1017
BclI T'GATC_A
Cuts at: 0 926 1157 1590
Size: 926 231 433
Fragments arranged by size:
926 433 231
BfrBI ATG'CAT
Cuts at: 0 3 1590
Size: 3 1587
BglI GCCn_nnn'nGGC
Cuts at: 0 1380 1590
Size: 1380 210
Bme1580I G_kGCm'C
Cuts at: 0 32 83 172 1117 1590
Size: 32 51 89 945 473
Fragments arranged by size:
945 473 89 51 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 888 1026 1407 1445 1590
Size: 888 138 381 38 145
Fragments arranged by size:
888 381 145 138 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1427 1590
Size: 1427 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 442 1048 1318 1324 1590
Size: 343 99 606 270 6 266
Fragments arranged by size:
606 343 270 266 99 6
Bpu10I CC'TnA_GC
Cuts at: 0 1354 1590
Size: 1354 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 1566 1590
Size: 861 705 24
Fragments arranged by size:
861 705 24
BsaAI yAC'GTr
Cuts at: 0 555 1590
Size: 555 1035
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 1590
Size: 466 30 1094
Fragments arranged by size:
1094 466 30
BseYI C'CCAG_C
Cuts at: 0 172 1222 1590
Size: 172 1050 368
Fragments arranged by size:
1050 368 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1590
Size: 96 1494
BsiHKAI G_wGCw'C
Cuts at: 0 83 554 1413 1461 1590
Size: 83 471 859 48 129
Fragments arranged by size:
859 471 129 83 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 572 1590
Size: 572 1018
Bsp1286I G_dGCh'C
Cuts at: 0 32 83 172 359 554 953 1117 1310
Size: 32 51 89 187 195 399 164 193
Cuts at: 1310 1413 1461 1590
Size: 103 48 129
Fragments arranged by size:
399 195 193 187 164 129 103 89
51 48 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 1590
Size: 280 1310
BsrDI GCAATG_nn'
Cuts at: 0 83 594 1590
Size: 83 511 996
Fragments arranged by size:
996 511 83
BstYI r'GATC_y
Cuts at: 0 1575 1590
Size: 1575 15
Bsu36I CC'TnA_GG
Cuts at: 0 1244 1376 1590
Size: 1244 132 214
Fragments arranged by size:
1244 214 132
BtgI C'CryG_G
Cuts at: 0 64 1590
Size: 64 1526
BtsI GCAGTG_nn'
Cuts at: 0 1180 1590
Size: 1180 410
DraI TTT'AAA
Cuts at: 0 938 1590
Size: 938 652
DraIII CAC_nnn'GTG
Cuts at: 0 325 1590
Size: 325 1265
EaeI y'GGCC_r
Cuts at: 0 1199 1590
Size: 1199 391
EcoICRI GAG'CTC
Cuts at: 0 1411 1459 1590
Size: 1411 48 131
Fragments arranged by size:
1411 131 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 442 610 1048 1318 1324 1544 1590
Size: 343 99 168 438 270 6 220 46
Fragments arranged by size:
438 343 270 220 168 99 46 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 1496 1590
Size: 124 1372 94
Fragments arranged by size:
1372 124 94
EcoO109I rG'GnC_Cy
Cuts at: 0 28 29 169 1248 1590
Size: 28 1 140 1079 342
Fragments arranged by size:
1079 342 140 28 1
EcoRI G'AATT_C
Cuts at: 0 1133 1590
Size: 1133 457
EcoRV GAT'ATC
Cuts at: 0 645 1590
Size: 645 945
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 854 886 1407 1439 1590
Size: 550 32 272 32 521 32 151
Fragments arranged by size:
550 521 272 151 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1590
Size: 452 1138
MscI TGG'CCA
Cuts at: 0 1201 1590
Size: 1201 389
MslI CAynn'nnrTG
Cuts at: 0 686 1049 1590
Size: 686 363 541
Fragments arranged by size:
686 541 363
MspA1I CmG'CkG
Cuts at: 0 829 1011 1590
Size: 829 182 579
Fragments arranged by size:
829 579 182
NdeI CA'TA_TG
Cuts at: 0 969 1590
Size: 969 621
NsiI A_TGCA'T
Cuts at: 0 5 1590
Size: 5 1585
NspI r_CATG'y
Cuts at: 0 183 294 388 1287 1330 1590
Size: 183 111 94 899 43 260
Fragments arranged by size:
899 260 183 111 94 43
PciI A'CATG_T
Cuts at: 0 384 1590
Size: 384 1206
PmlI CAC'GTG
Cuts at: 0 555 1590
Size: 555 1035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 1590
Size: 130 32 1428
Fragments arranged by size:
1428 130 32
PpuMI rG'GwC_Cy
Cuts at: 0 1248 1590
Size: 1248 342
PshAI GACnn'nnGTC
Cuts at: 0 1492 1590
Size: 1492 98
PspOMI G'GGCC_C
Cuts at: 0 28 168 1113 1590
Size: 28 140 945 477
Fragments arranged by size:
945 477 140 28
PspXI vC'TCGA_Gb
Cuts at: 0 1460 1590
Size: 1460 130
PstI C_TGCA'G
Cuts at: 0 137 160 1590
Size: 137 23 1430
Fragments arranged by size:
1430 137 23
PvuII CAG'CTG
Cuts at: 0 829 1011 1590
Size: 829 182 579
Fragments arranged by size:
829 579 182
SacI G_AGCT'C
Cuts at: 0 1413 1461 1590
Size: 1413 48 129
Fragments arranged by size:
1413 129 48
SfcI C'TryA_G
Cuts at: 0 133 156 632 1590
Size: 133 23 476 958
Fragments arranged by size:
958 476 133 23
SmlI C'TyrA_G
Cuts at: 0 840 1267 1460 1581 1590
Size: 840 427 193 121 9
Fragments arranged by size:
840 427 193 121 9
SphI G_CATG'C
Cuts at: 0 1287 1590
Size: 1287 303
StyI C'CwwG_G
Cuts at: 0 1097 1590
Size: 1097 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1406 1590
Size: 1406 184
TatI w'GTAC_w
Cuts at: 0 1343 1590
Size: 1343 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 443 475 1590
Size: 443 32 1115
Fragments arranged by size:
1115 443 32
XhoI C'TCGA_G
Cuts at: 0 1460 1590
Size: 1460 130
XmnI GAAnn'nnTTC
Cuts at: 0 142 1590
Size: 142 1448
Enzymes that do cut:
AcuI AflII AflIII AhdI ApaI ApaLI ApoI AvaI
BamHI BanII BcgI BciVI BclI BfrBI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I
NdeI NsiI NspI PciI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SfcI SmlI SphI
StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BglII BlpI BmgBI BmtI
BsaI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DrdI
EagI EarI EciI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XmaI ZraI