(Linear) (Six-base) MAPSORT of: KIBB5836.seq  Check: 1238  from: 1  to: 1443



KIBB5836.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AatII G_ACGT'C

Cuts at:      0      19    1443 
   Size:         19    1424
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365    1443 
   Size:        365    1078
 
AflII C'TTAA_G

Cuts at:      0     291    1443 
   Size:        291    1152
 
AleI CACnn'nnGTG

Cuts at:      0    1016    1443 
   Size:       1016     427
 
ApoI r'AATT_y

Cuts at:      0     399     525     693     920    1078    1443 
   Size:        399     126     168     227     158     365

  Fragments arranged by size: 

                 399     365     227     168     158     126
 
AseI AT'TA_AT

Cuts at:      0     281    1443 
   Size:        281    1162
 
BanII G_rGCy'C

Cuts at:      0    1059    1443 
   Size:       1059     384
 
BbvCI CC'TCA_GC

Cuts at:      0      44    1443 
   Size:         44    1399
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1204    1443 
   Size:       1204     239
 
BglII A'GATC_T

Cuts at:      0     816    1443 
   Size:        816     627
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155    1443 
   Size:        155    1288
 
Bpu10I CC'TnA_GC

Cuts at:      0      44    1443 
   Size:         44    1399
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225    1443 
   Size:        225    1218
 
BsaHI Gr'CG_yC

Cuts at:      0      16    1443 
   Size:         16    1427
 
Bsp1286I G_dGCh'C

Cuts at:      0    1059    1443 
   Size:       1059     384
 
BsrBI CCG'CTC

Cuts at:      0     571    1443 
   Size:        571     872
 
BstEII G'GTnAC_C

Cuts at:      0     651    1443 
   Size:        651     792
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     968    1443 
   Size:        968     475
 
BstYI r'GATC_y

Cuts at:      0     183     816    1443 
   Size:        183     633     627

  Fragments arranged by size: 

                 633     627     183
 
BtgI C'CryG_G

Cuts at:      0     140    1443 
   Size:        140    1303
 
BtsI GCAGTG_nn'

Cuts at:      0    1079    1443 
   Size:       1079     364
 
DraI TTT'AAA

Cuts at:      0     530    1443 
   Size:        530     913
 
EaeI y'GGCC_r

Cuts at:      0     101     932    1443 
   Size:        101     831     511

  Fragments arranged by size: 

                 831     511     101
 
EarI CTCTTCn'nnn_

Cuts at:      0     341    1187    1443 
   Size:        341     846     256

  Fragments arranged by size: 

                 846     341     256
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     365    1443 
   Size:        155     210    1078

  Fragments arranged by size: 

                1078     210     155
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     779    1443 
   Size:        779     664
 
EcoRI G'AATT_C

Cuts at:      0     399    1078    1443 
   Size:        399     679     365

  Fragments arranged by size: 

                 679     399     365
 
HaeII r_GCGC'y

Cuts at:      0    1235    1443 
   Size:       1235     208
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1202    1234    1443 
   Size:       1202      32     209

  Fragments arranged by size: 

                1202     209      32
 
HincII GTy'rAC

Cuts at:      0    1400    1443 
   Size:       1400      43
 
HpaI GTT'AAC

Cuts at:      0    1400    1443 
   Size:       1400      43
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335    1443 
   Size:        335    1108
 
MscI TGG'CCA

Cuts at:      0     934    1443 
   Size:        934     509
 
MslI CAynn'nnrTG

Cuts at:      0     640     800     828    1016    1202    1443 
   Size:        640     160      28     188     186     241

  Fragments arranged by size: 

                 640     241     188     186     160      28
 
MspA1I CmG'CkG

Cuts at:      0      43    1443 
   Size:         43    1400
 
NcoI C'CATG_G

Cuts at:      0     140    1443 
   Size:        140    1303
 
PacI TTA_AT'TAA

Cuts at:      0     368    1443 
   Size:        368    1075
 
PshAI GACnn'nnGTC

Cuts at:      0    1310    1443 
   Size:       1310     133
 
PstI C_TGCA'G

Cuts at:      0      91    1443 
   Size:         91    1352
 
PvuII CAG'CTG

Cuts at:      0      43    1443 
   Size:         43    1400
 
SapI GCTCTTCn'nnn_

Cuts at:      0     341    1187    1443 
   Size:        341     846     256

  Fragments arranged by size: 

                 846     341     256
 
ScaI AGT'ACT

Cuts at:      0    1360    1443 
   Size:       1360      83
 
SfcI C'TryA_G

Cuts at:      0      87    1443 
   Size:         87    1356
 
SmlI C'TyrA_G

Cuts at:      0     240     291    1443 
   Size:        240      51    1152

  Fragments arranged by size: 

                1152     240      51
 
SspI AAT'ATT

Cuts at:      0    1048    1443 
   Size:       1048     395
 
StuI AGG'CCT

Cuts at:      0    1064    1443 
   Size:       1064     379
 
StyI C'CwwG_G

Cuts at:      0     140     935    1065    1308    1443 
   Size:        140     795     130     243     135

  Fragments arranged by size: 

                 795     243     140     135     130
 
TatI w'GTAC_w

Cuts at:      0    1358    1443 
   Size:       1358      85
 
XmnI GAAnn'nnTTC

Cuts at:      0      82     626     710    1339    1443 
   Size:         82     544      84     629     104

  Fragments arranged by size: 

                 629     544     104      84      82
 
ZraI GAC'GTC

Cuts at:      0      17    1443 
   Size:         17    1426
 

 Enzymes that do cut:

    AatII     AcuI    AflII     AleI     ApoI     AseI    BanII    BbvCI
    BciVI    BglII     BpmI   Bpu10I    BpuEI    BsaHI Bsp1286I    BsrBI
   BstEII    BstXI    BstYI     BtgI     BtsI     DraI     EaeI     EarI
  Eco57MI    EcoNI    EcoRI    HaeII    Hin4I   HincII     HpaI     MmeI
     MscI     MslI   MspA1I     NcoI     PacI    PshAI     PstI    PvuII
     SapI     ScaI     SfcI     SmlI     SspI     StuI     StyI     TatI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI