Restriction map for
pF1KB5837
(Linear) (Six-base) MAPSORT of: KIBB5837.seq Check: 3144 from: 1 to: 720
KIBB5837.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 394 720
Size: 394 326
Acc65I G'GTAC_C
Cuts at: 0 148 720
Size: 148 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 720
Size: 205 515
AhdI GACnn_n'nnGTC
Cuts at: 0 306 720
Size: 306 414
AleI CACnn'nnGTG
Cuts at: 0 457 460 720
Size: 457 3 260
Fragments arranged by size:
457 260 3
ApaI G_GGCC'C
Cuts at: 0 616 720
Size: 616 104
ApaLI G'TGCA_C
Cuts at: 0 74 720
Size: 74 646
ApoI r'AATT_y
Cuts at: 0 190 720
Size: 190 530
AvaI C'yCGr_G
Cuts at: 0 121 720
Size: 121 599
BamHI G'GATC_C
Cuts at: 0 26 720
Size: 26 694
BanI G'GyrC_C
Cuts at: 0 148 720
Size: 148 572
BanII G_rGCy'C
Cuts at: 0 271 616 720
Size: 271 345 104
Fragments arranged by size:
345 271 104
BbvCI CC'TCA_GC
Cuts at: 0 277 479 545 720
Size: 277 202 66 175
Fragments arranged by size:
277 202 175 66
BglI GCCn_nnn'nGGC
Cuts at: 0 709 720
Size: 709 11
Bme1580I G_kGCm'C
Cuts at: 0 78 616 720
Size: 78 538 104
Fragments arranged by size:
538 104 78
BmgBI CAC'GTC
Cuts at: 0 180 413 427 720
Size: 180 233 14 293
Fragments arranged by size:
293 233 180 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 720
Size: 662 58
Bpu10I CC'TnA_GC
Cuts at: 0 277 479 545 720
Size: 277 202 66 175
Fragments arranged by size:
277 202 175 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 720
Size: 625 95
BsaAI yAC'GTr
Cuts at: 0 79 720
Size: 79 641
BsaWI w'CCGG_w
Cuts at: 0 429 720
Size: 429 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 51 81 720
Size: 51 30 639
Fragments arranged by size:
639 51 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 659 720
Size: 659 61
BseYI C'CCAG_C
Cuts at: 0 264 499 597 602 720
Size: 264 235 98 5 118
Fragments arranged by size:
264 235 118 98 5
BsiHKAI G_wGCw'C
Cuts at: 0 78 720
Size: 78 642
Bsp1286I G_dGCh'C
Cuts at: 0 78 271 616 720
Size: 78 193 345 104
Fragments arranged by size:
345 193 104 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 394 720
Size: 394 326
BsrFI r'CCGG_y
Cuts at: 0 9 252 618 720
Size: 9 243 366 102
Fragments arranged by size:
366 243 102 9
BssSI C'ACGA_G
Cuts at: 0 628 720
Size: 628 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 459 720
Size: 459 261
BstYI r'GATC_y
Cuts at: 0 26 720
Size: 26 694
Bsu36I CC'TnA_GG
Cuts at: 0 224 720
Size: 224 496
BtgI C'CryG_G
Cuts at: 0 22 102 608 720
Size: 22 80 506 112
Fragments arranged by size:
506 112 80 22
DraIII CAC_nnn'GTG
Cuts at: 0 82 720
Size: 82 638
EaeI y'GGCC_r
Cuts at: 0 329 720
Size: 329 391
EarI CTCTTCn'nnn_
Cuts at: 0 121 720
Size: 121 599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 662 720
Size: 205 457 58
Fragments arranged by size:
457 205 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 548 720
Size: 548 172
HaeII r_GCGC'y
Cuts at: 0 277 720
Size: 277 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 292 324 720
Size: 98 32 162 32 396
Fragments arranged by size:
396 162 98 32 32
KpnI G_GTAC'C
Cuts at: 0 152 720
Size: 152 568
MslI CAynn'nnrTG
Cuts at: 0 349 457 460 720
Size: 349 108 3 260
Fragments arranged by size:
349 260 108 3
MspA1I CmG'CkG
Cuts at: 0 24 264 720
Size: 24 240 456
Fragments arranged by size:
456 240 24
NcoI C'CATG_G
Cuts at: 0 608 720
Size: 608 112
NspI r_CATG'y
Cuts at: 0 469 720
Size: 469 251
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 720
Size: 609 111
PmlI CAC'GTG
Cuts at: 0 79 720
Size: 79 641
PspOMI G'GGCC_C
Cuts at: 0 612 720
Size: 612 108
PvuII CAG'CTG
Cuts at: 0 264 720
Size: 264 456
SacII CC_GC'GG
Cuts at: 0 25 720
Size: 25 695
SphI G_CATG'C
Cuts at: 0 469 720
Size: 469 251
StyI C'CwwG_G
Cuts at: 0 326 608 720
Size: 326 282 112
Fragments arranged by size:
326 282 112
Tth111I GACn'n_nGTC
Cuts at: 0 425 720
Size: 425 295
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbvCI BglI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstXI BstYI Bsu36I
BtgI DraIII EaeI EarI Eco57MI EcoNI HaeII Hin4I
KpnI MslI MspA1I NcoI NspI PflMI PmlI PspOMI
PvuII SacII SphI StyI Tth111I
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AlfI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BpuEI BsaBI BsaHI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
TstI XbaI XcmI XhoI XmaI XmnI ZraI