(Linear) (Six-base) MAPSORT of: KIBB5845.seq  Check: 4135  from: 1  to: 417



KIBB5845.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     401     417 
   Size:        401      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     344     358     417 
   Size:        283      61      14      59

  Fragments arranged by size: 

                 283      61      59      14
 
AflIII A'CryG_T

Cuts at:      0     109     417 
   Size:        109     308
 
AlwNI CAG_nnn'CTG

Cuts at:      0     322     417 
   Size:        322      95
 
ApaI G_GGCC'C

Cuts at:      0     133     417 
   Size:        133     284
 
BanII G_rGCy'C

Cuts at:      0     133     417 
   Size:        133     284
 
Bme1580I G_kGCm'C

Cuts at:      0     133     247     417 
   Size:        133     114     170

  Fragments arranged by size: 

                 170     133     114
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132     417 
   Size:        132     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     195     417 
   Size:        172      23     222

  Fragments arranged by size: 

                 222     172      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     417 
   Size:        118     299
 
BseYI C'CCAG_C

Cuts at:      0     161     179     417 
   Size:        161      18     238

  Fragments arranged by size: 

                 238     161      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     247     417 
   Size:        133     114     170

  Fragments arranged by size: 

                 170     133     114
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     417 
   Size:        401      16
 
BsrFI r'CCGG_y

Cuts at:      0     332     417 
   Size:        332      85
 
BstYI r'GATC_y

Cuts at:      0     188     417 
   Size:        188     229
 
Bsu36I CC'TnA_GG

Cuts at:      0     281     417 
   Size:        281     136
 
EaeI y'GGCC_r

Cuts at:      0     372     417 
   Size:        372      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     195     283     344     358     417 
   Size:        172      23      88      61      14      59

  Fragments arranged by size: 

                 172      88      61      59      23      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     130     417 
   Size:        129       1     287

  Fragments arranged by size: 

                 287     129       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     417 
   Size:        277      32     108

  Fragments arranged by size: 

                 277     108      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     417 
   Size:         94     323
 
MspA1I CmG'CkG

Cuts at:      0     183     319     417 
   Size:        183     136      98

  Fragments arranged by size: 

                 183     136      98
 
NspI r_CATG'y

Cuts at:      0     113     417 
   Size:        113     304
 
PciI A'CATG_T

Cuts at:      0     109     417 
   Size:        109     308
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12     417 
   Size:         12     405
 
PspOMI G'GGCC_C

Cuts at:      0     129     417 
   Size:        129     288
 
PstI C_TGCA'G

Cuts at:      0     188     417 
   Size:        188     229
 
PvuII CAG'CTG

Cuts at:      0     183     319     417 
   Size:        183     136      98

  Fragments arranged by size: 

                 183     136      98
 
SfcI C'TryA_G

Cuts at:      0     184     417 
   Size:        184     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     132     417 
   Size:        132     285
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     199     417 
   Size:        199     218
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApaI    BanII Bme1580I     BmrI
     BpmI    BseRI    BseYI Bsp1286I    BspMI    BsrFI    BstYI   Bsu36I
     EaeI  Eco57MI EcoO109I    Hin4I     MmeI   MspA1I     NspI     PciI
    PflMI   PspOMI     PstI    PvuII     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI