Restriction map for
pF1KB5848
(Linear) (Six-base) MAPSORT of: KIBB5848.seq Check: 4586 from: 1 to: 1458
KIBB5848.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 237 1244 1458
Size: 237 1007 214
Fragments arranged by size:
1007 237 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1458
Size: 640 818
AfeI AGC'GCT
Cuts at: 0 109 1458
Size: 109 1349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 1458
Size: 599 32 827
Fragments arranged by size:
827 599 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 897 1458
Size: 576 321 561
Fragments arranged by size:
576 561 321
ApoI r'AATT_y
Cuts at: 0 490 559 638 649 946 1169 1202 1458
Size: 490 69 79 11 297 223 33 256
Fragments arranged by size:
490 297 256 223 79 69 33 11
BanII G_rGCy'C
Cuts at: 0 121 517 1458
Size: 121 396 941
Fragments arranged by size:
941 396 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 1458
Size: 628 830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1269 1303 1458
Size: 1269 34 155
Fragments arranged by size:
1269 155 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1089 1458
Size: 1089 369
BclI T'GATC_A
Cuts at: 0 377 552 929 1458
Size: 377 175 377 529
Fragments arranged by size:
529 377 377 175
BfrBI ATG'CAT
Cuts at: 0 837 1458
Size: 837 621
BglI GCCn_nnn'nGGC
Cuts at: 0 46 1458
Size: 46 1412
BlpI GC'TnA_GC
Cuts at: 0 869 1458
Size: 869 589
BmtI G_CTAG'C
Cuts at: 0 877 1458
Size: 877 581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 919 1458
Size: 598 321 539
Fragments arranged by size:
598 539 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 519 1458
Size: 475 44 939
Fragments arranged by size:
939 475 44
BsaBI GATnn'nnATC
Cuts at: 0 428 1458
Size: 428 1030
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 390 420 557 587 1458
Size: 390 30 137 30 871
Fragments arranged by size:
871 390 137 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1168 1390 1458
Size: 1168 222 68
Fragments arranged by size:
1168 222 68
BseYI C'CCAG_C
Cuts at: 0 170 1458
Size: 170 1288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 616 1458
Size: 142 474 842
Fragments arranged by size:
842 474 142
BsmI GAATG_Cn'
Cuts at: 0 667 1458
Size: 667 791
Bsp1286I G_dGCh'C
Cuts at: 0 121 517 1458
Size: 121 396 941
Fragments arranged by size:
941 396 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 970 1458
Size: 970 488
BsrDI GCAATG_nn'
Cuts at: 0 533 781 1458
Size: 533 248 677
Fragments arranged by size:
677 533 248
BsrFI r'CCGG_y
Cuts at: 0 18 1458
Size: 18 1440
BsrGI T'GTAC_A
Cuts at: 0 888 1196 1458
Size: 888 308 262
Fragments arranged by size:
888 308 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 1458
Size: 165 1293
BstYI r'GATC_y
Cuts at: 0 597 1458
Size: 597 861
BstZ17I GTA'TAC
Cuts at: 0 238 1245 1458
Size: 238 1007 213
Fragments arranged by size:
1007 238 213
Bsu36I CC'TnA_GG
Cuts at: 0 700 1458
Size: 700 758
BtsI GCAGTG_nn'
Cuts at: 0 235 1458
Size: 235 1223
EaeI y'GGCC_r
Cuts at: 0 952 1458
Size: 952 506
EarI CTCTTCn'nnn_
Cuts at: 0 268 431 1040 1458
Size: 268 163 609 418
Fragments arranged by size:
609 418 268 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 640 919 1458
Size: 598 42 279 539
Fragments arranged by size:
598 539 279 42
EcoRI G'AATT_C
Cuts at: 0 490 1458
Size: 490 968
EcoRV GAT'ATC
Cuts at: 0 1067 1458
Size: 1067 391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1069 1458
Size: 1069 389
HaeII r_GCGC'y
Cuts at: 0 38 111 1458
Size: 38 73 1347
Fragments arranged by size:
1347 73 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 1458
Size: 656 32 770
Fragments arranged by size:
770 656 32
HindIII A'AGCT_T
Cuts at: 0 1282 1458
Size: 1282 176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1458
Size: 1249 209
MscI TGG'CCA
Cuts at: 0 954 1458
Size: 954 504
MslI CAynn'nnrTG
Cuts at: 0 728 1458
Size: 728 730
MspA1I CmG'CkG
Cuts at: 0 165 1458
Size: 165 1293
NaeI GCC'GGC
Cuts at: 0 20 1458
Size: 20 1438
NgoMIV G'CCGG_C
Cuts at: 0 18 1458
Size: 18 1440
NheI G'CTAG_C
Cuts at: 0 873 1458
Size: 873 585
NsiI A_TGCA'T
Cuts at: 0 839 1458
Size: 839 619
NspI r_CATG'y
Cuts at: 0 1428 1458
Size: 1428 30
PasI CC'CwG_GG
Cuts at: 0 114 1458
Size: 114 1344
PfoI T'CCnGG_A
Cuts at: 0 575 896 1458
Size: 575 321 562
Fragments arranged by size:
575 562 321
PstI C_TGCA'G
Cuts at: 0 967 1458
Size: 967 491
RsrII CG'GwC_CG
Cuts at: 0 84 1458
Size: 84 1374
SapI GCTCTTCn'nnn_
Cuts at: 0 268 1040 1458
Size: 268 772 418
Fragments arranged by size:
772 418 268
SfcI C'TryA_G
Cuts at: 0 963 1458
Size: 963 495
SgrAI Cr'CCGG_yG
Cuts at: 0 18 1458
Size: 18 1440
SmlI C'TyrA_G
Cuts at: 0 454 498 1458
Size: 454 44 960
Fragments arranged by size:
960 454 44
SspI AAT'ATT
Cuts at: 0 755 1458
Size: 755 703
StuI AGG'CCT
Cuts at: 0 460 1458
Size: 460 998
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 1458
Size: 72 1386
TatI w'GTAC_w
Cuts at: 0 888 1115 1196 1458
Size: 888 227 81 262
Fragments arranged by size:
888 262 227 81
XbaI T'CTAG_A
Cuts at: 0 1398 1458
Size: 1398 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1236 1458
Size: 1236 222
XmnI GAAnn'nnTTC
Cuts at: 0 1136 1458
Size: 1136 322
Enzymes that do cut:
AccI AcuI AfeI AloI AlwNI ApoI BanII BbsI
BcgI BciVI BclI BfrBI BglI BlpI BmtI BpmI
BpuEI BsaBI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I
BtsI EaeI EarI Eco57MI EcoRI EcoRV FalI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV
NheI NsiI NspI PasI PfoI PstI RsrII SapI
SfcI SgrAI SmlI SspI StuI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BglII Bme1580I
BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstXI BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NotI NruI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI StyI SwaI TstI Tth111I XhoI
XmaI ZraI