Restriction map for
pF1KB5849
(Linear) (Six-base) MAPSORT of: KIBB5849.seq Check: 3158 from: 1 to: 738
KIBB5849.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AfeI AGC'GCT
Cuts at: 0 637 738
Size: 637 101
AleI CACnn'nnGTG
Cuts at: 0 283 738
Size: 283 455
AlwNI CAG_nnn'CTG
Cuts at: 0 84 181 738
Size: 84 97 557
Fragments arranged by size:
557 97 84
AvaI C'yCGr_G
Cuts at: 0 504 595 738
Size: 504 91 143
Fragments arranged by size:
504 143 91
BanI G'GyrC_C
Cuts at: 0 568 738
Size: 568 170
BanII G_rGCy'C
Cuts at: 0 448 587 596 602 738
Size: 448 139 9 6 136
Fragments arranged by size:
448 139 136 9 6
BbvCI CC'TCA_GC
Cuts at: 0 254 738
Size: 254 484
BclI T'GATC_A
Cuts at: 0 287 738
Size: 287 451
Bme1580I G_kGCm'C
Cuts at: 0 571 738
Size: 571 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 738
Size: 315 423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 738
Size: 462 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 109 738
Size: 58 51 629
Fragments arranged by size:
629 58 51
Bpu10I CC'TnA_GC
Cuts at: 0 254 738
Size: 254 484
BsaWI w'CCGG_w
Cuts at: 0 302 738
Size: 302 436
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 454 738
Size: 243 211 284
Fragments arranged by size:
284 243 211
BseYI C'CCAG_C
Cuts at: 0 130 406 738
Size: 130 276 332
Fragments arranged by size:
332 276 130
BsiEI CG_ry'CG
Cuts at: 0 303 738
Size: 303 435
BsmI GAATG_Cn'
Cuts at: 0 403 738
Size: 403 335
Bsp1286I G_dGCh'C
Cuts at: 0 448 571 587 596 602 738
Size: 448 123 16 9 6 136
Fragments arranged by size:
448 136 123 16 9 6
BsrGI T'GTAC_A
Cuts at: 0 479 738
Size: 479 259
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 738
Size: 84 654
BstEII G'GTnAC_C
Cuts at: 0 453 738
Size: 453 285
BstYI r'GATC_y
Cuts at: 0 529 710 738
Size: 529 181 28
Fragments arranged by size:
529 181 28
Bsu36I CC'TnA_GG
Cuts at: 0 431 738
Size: 431 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 738
Size: 156 582
BtsI GCAGTG_nn'
Cuts at: 0 85 547 738
Size: 85 462 191
Fragments arranged by size:
462 191 85
EaeI y'GGCC_r
Cuts at: 0 44 738
Size: 44 694
EarI CTCTTCn'nnn_
Cuts at: 0 88 220 738
Size: 88 132 518
Fragments arranged by size:
518 132 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 640 738
Size: 640 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 109 738
Size: 58 51 629
Fragments arranged by size:
629 58 51
FspI TGC'GCA
Cuts at: 0 370 738
Size: 370 368
HaeII r_GCGC'y
Cuts at: 0 639 738
Size: 639 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 571 603 738
Size: 521 32 18 32 135
Fragments arranged by size:
521 135 32 32 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 738
Size: 299 439
MscI TGG'CCA
Cuts at: 0 46 738
Size: 46 692
MslI CAynn'nnrTG
Cuts at: 0 283 738
Size: 283 455
MspA1I CmG'CkG
Cuts at: 0 216 426 738
Size: 216 210 312
Fragments arranged by size:
312 216 210
NdeI CA'TA_TG
Cuts at: 0 282 738
Size: 282 456
NspI r_CATG'y
Cuts at: 0 633 738
Size: 633 105
PstI C_TGCA'G
Cuts at: 0 529 554 738
Size: 529 25 184
Fragments arranged by size:
529 184 25
SfcI C'TryA_G
Cuts at: 0 525 550 738
Size: 525 25 188
Fragments arranged by size:
525 188 25
SmaI CCC'GGG
Cuts at: 0 506 738
Size: 506 232
StuI AGG'CCT
Cuts at: 0 670 738
Size: 670 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 510 738
Size: 166 344 228
Fragments arranged by size:
344 228 166
TatI w'GTAC_w
Cuts at: 0 479 738
Size: 479 259
XmaI C'CCGG_G
Cuts at: 0 504 738
Size: 504 234
Enzymes that do cut:
AfeI AleI AlwNI AvaI BanI BanII BbvCI BclI
Bme1580I BmrI BplI BpmI Bpu10I BsaWI BseRI BseYI
BsiEI BsmI Bsp1286I BsrGI BstAPI BstEII BstYI Bsu36I
BtgZI BtsI EaeI EarI EciI Eco57MI FspI HaeII
Hin4I MmeI MscI MslI MspA1I NdeI NspI PstI
SfcI SmaI StuI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstXI BstZ17I BtgI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI