Restriction map for
pF1KB5852
(Linear) (Six-base) MAPSORT of: KIBB5852.seq Check: 6420 from: 1 to: 1407
KIBB5852.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 0 89 1407
Size: 89 1318
AccI GT'mk_AC
Cuts at: 0 846 1407
Size: 846 561
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1407
Size: 650 757
AgeI A'CCGG_T
Cuts at: 0 323 1407
Size: 323 1084
ApaLI G'TGCA_C
Cuts at: 0 595 1407
Size: 595 812
ApoI r'AATT_y
Cuts at: 0 119 1045 1060 1074 1407
Size: 119 926 15 14 333
Fragments arranged by size:
926 333 119 15 14
AseI AT'TA_AT
Cuts at: 0 250 1407
Size: 250 1157
BanI G'GyrC_C
Cuts at: 0 313 1407
Size: 313 1094
BanII G_rGCy'C
Cuts at: 0 434 1179 1407
Size: 434 745 228
Fragments arranged by size:
745 434 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 159 1407
Size: 89 70 1248
Fragments arranged by size:
1248 89 70
Bme1580I G_kGCm'C
Cuts at: 0 599 1407
Size: 599 808
BmgBI CAC'GTC
Cuts at: 0 594 1373 1407
Size: 594 779 34
Fragments arranged by size:
779 594 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 1175 1407
Size: 1015 160 232
Fragments arranged by size:
1015 232 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1407
Size: 448 959
Bpu10I CC'TnA_GC
Cuts at: 0 1265 1407
Size: 1265 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 967 1119 1407
Size: 286 681 152 288
Fragments arranged by size:
681 288 286 152
BsaBI GATnn'nnATC
Cuts at: 0 812 1407
Size: 812 595
BsaHI Gr'CG_yC
Cuts at: 0 86 1407
Size: 86 1321
BsaWI w'CCGG_w
Cuts at: 0 323 1407
Size: 323 1084
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 738 768 1407
Size: 738 30 639
Fragments arranged by size:
738 639 30
BsiHKAI G_wGCw'C
Cuts at: 0 434 599 1274 1407
Size: 434 165 675 133
Fragments arranged by size:
675 434 165 133
BsiWI C'GTAC_G
Cuts at: 0 130 1407
Size: 130 1277
Bsp1286I G_dGCh'C
Cuts at: 0 434 599 1179 1274 1407
Size: 434 165 580 95 133
Fragments arranged by size:
580 434 165 133 95
BsrFI r'CCGG_y
Cuts at: 0 323 1407
Size: 323 1084
BsrGI T'GTAC_A
Cuts at: 0 68 1407
Size: 68 1339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 1407
Size: 63 1344
BstYI r'GATC_y
Cuts at: 0 219 560 1318 1407
Size: 219 341 758 89
Fragments arranged by size:
758 341 219 89
BtgI C'CryG_G
Cuts at: 0 261 913 1407
Size: 261 652 494
Fragments arranged by size:
652 494 261
BtsI GCAGTG_nn'
Cuts at: 0 1100 1407
Size: 1100 307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 614 649 1407
Size: 614 35 758
Fragments arranged by size:
758 614 35
EaeI y'GGCC_r
Cuts at: 0 41 346 458 1407
Size: 41 305 112 949
Fragments arranged by size:
949 305 112 41
EarI CTCTTCn'nnn_
Cuts at: 0 532 1407
Size: 532 875
EcoICRI GAG'CTC
Cuts at: 0 432 1407
Size: 432 975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 650 1407
Size: 448 202 757
Fragments arranged by size:
757 448 202
EcoO109I rG'GnC_Cy
Cuts at: 0 1101 1407
Size: 1101 306
EcoRI G'AATT_C
Cuts at: 0 119 1060 1407
Size: 119 941 347
Fragments arranged by size:
941 347 119
HaeII r_GCGC'y
Cuts at: 0 235 1407
Size: 235 1172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 416 448 1407
Size: 416 32 959
Fragments arranged by size:
959 416 32
HincII GTy'rAC
Cuts at: 0 1385 1407
Size: 1385 22
HindIII A'AGCT_T
Cuts at: 0 141 1407
Size: 141 1266
HpaI GTT'AAC
Cuts at: 0 1385 1407
Size: 1385 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1407
Size: 781 626
MscI TGG'CCA
Cuts at: 0 348 460 1407
Size: 348 112 947
Fragments arranged by size:
947 348 112
MslI CAynn'nnrTG
Cuts at: 0 64 502 1407
Size: 64 438 905
Fragments arranged by size:
905 438 64
NcoI C'CATG_G
Cuts at: 0 913 1407
Size: 913 494
NspI r_CATG'y
Cuts at: 0 63 1407
Size: 63 1344
PasI CC'CwG_GG
Cuts at: 0 6 1407
Size: 6 1401
PflMI CCAn_nnn'nTGG
Cuts at: 0 938 1407
Size: 938 469
PfoI T'CCnGG_A
Cuts at: 0 1170 1407
Size: 1170 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 1407
Size: 568 32 807
Fragments arranged by size:
807 568 32
PpuMI rG'GwC_Cy
Cuts at: 0 1101 1407
Size: 1101 306
PsiI TTA'TAA
Cuts at: 0 1315 1407
Size: 1315 92
PstI C_TGCA'G
Cuts at: 0 470 1407
Size: 470 937
SacI G_AGCT'C
Cuts at: 0 434 1407
Size: 434 973
SfcI C'TryA_G
Cuts at: 0 212 466 1342 1407
Size: 212 254 876 65
Fragments arranged by size:
876 254 212 65
SmlI C'TyrA_G
Cuts at: 0 301 946 1134 1407
Size: 301 645 188 273
Fragments arranged by size:
645 301 273 188
SspI AAT'ATT
Cuts at: 0 790 1407
Size: 790 617
StuI AGG'CCT
Cuts at: 0 1116 1407
Size: 1116 291
StyI C'CwwG_G
Cuts at: 0 913 1407
Size: 913 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 69 750 1407
Size: 69 681 657
Fragments arranged by size:
681 657 69
TatI w'GTAC_w
Cuts at: 0 68 1407
Size: 68 1339
XbaI T'CTAG_A
Cuts at: 0 1050 1407
Size: 1050 357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 356 1407
Size: 36 320 1051
Fragments arranged by size:
1051 320 36
ZraI GAC'GTC
Cuts at: 0 87 1407
Size: 87 1320
Enzymes that do cut:
AatII AccI AcuI AgeI ApaLI ApoI AseI BanI
BanII BbsI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI Bsp1286I BsrFI
BsrGI BstXI BstYI BtgI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI NcoI NspI PasI PflMI
PfoI PpiI PpuMI PsiI PstI SacI SfcI SmlI
SspI StuI StyI TaqII TatI XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI BsaI BsaAI BseRI
BseYI BsgI BsiEI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI
Tth111I XhoI XmaI XmnI