Restriction map for
pF1KB5853
(Linear) (Six-base) MAPSORT of: KIBB5853.seq Check: 1653 from: 1 to: 966
KIBB5853.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 796 875 966
Size: 796 79 91
Fragments arranged by size:
796 91 79
AfeI AGC'GCT
Cuts at: 0 268 966
Size: 268 698
AhdI GACnn_n'nnGTC
Cuts at: 0 174 966
Size: 174 792
AleI CACnn'nnGTG
Cuts at: 0 258 391 966
Size: 258 133 575
Fragments arranged by size:
575 258 133
AvrII C'CTAG_G
Cuts at: 0 741 966
Size: 741 225
BanII G_rGCy'C
Cuts at: 0 344 966
Size: 344 622
BbvCI CC'TCA_GC
Cuts at: 0 199 966
Size: 199 767
BmrI ACTGGGnnnn_n'
Cuts at: 0 276 966
Size: 276 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 966
Size: 526 440
Bpu10I CC'TnA_GC
Cuts at: 0 199 931 966
Size: 199 732 35
Fragments arranged by size:
732 199 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 921 966
Size: 921 45
BseYI C'CCAG_C
Cuts at: 0 146 356 460 471 966
Size: 146 210 104 11 495
Fragments arranged by size:
495 210 146 104 11
BsiHKAI G_wGCw'C
Cuts at: 0 17 966
Size: 17 949
BsmI GAATG_Cn'
Cuts at: 0 124 966
Size: 124 842
Bsp1286I G_dGCh'C
Cuts at: 0 17 344 966
Size: 17 327 622
Fragments arranged by size:
622 327 17
BsrDI GCAATG_nn'
Cuts at: 0 673 966
Size: 673 293
BstAPI GCAn_nnn'nTGC
Cuts at: 0 780 966
Size: 780 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 966
Size: 234 732
BstYI r'GATC_y
Cuts at: 0 905 966
Size: 905 61
Bsu36I CC'TnA_GG
Cuts at: 0 247 966
Size: 247 719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 124 966
Size: 124 842
BtsI GCAGTG_nn'
Cuts at: 0 119 466 966
Size: 119 347 500
Fragments arranged by size:
500 347 119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 570 605 833 868 966
Size: 570 35 228 35 98
Fragments arranged by size:
570 228 98 35 35
EaeI y'GGCC_r
Cuts at: 0 489 548 966
Size: 489 59 418
Fragments arranged by size:
489 418 59
EarI CTCTTCn'nnn_
Cuts at: 0 820 966
Size: 820 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 966
Size: 526 440
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 966
Size: 202 764
EcoO109I rG'GnC_Cy
Cuts at: 0 150 194 308 405 966
Size: 150 44 114 97 561
Fragments arranged by size:
561 150 114 97 44
HaeII r_GCGC'y
Cuts at: 0 270 966
Size: 270 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 358 390 503 535 966
Size: 160 32 166 32 113 32 431
Fragments arranged by size:
431 166 160 113 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 489 966
Size: 89 400 477
Fragments arranged by size:
477 400 89
MscI TGG'CCA
Cuts at: 0 491 550 966
Size: 491 59 416
Fragments arranged by size:
491 416 59
MslI CAynn'nnrTG
Cuts at: 0 258 391 966
Size: 258 133 575
Fragments arranged by size:
575 258 133
MspA1I CmG'CkG
Cuts at: 0 546 966
Size: 546 420
NdeI CA'TA_TG
Cuts at: 0 523 966
Size: 523 443
PfoI T'CCnGG_A
Cuts at: 0 17 158 966
Size: 17 141 808
Fragments arranged by size:
808 141 17
PpuMI rG'GwC_Cy
Cuts at: 0 150 194 308 405 966
Size: 150 44 114 97 561
Fragments arranged by size:
561 150 114 97 44
PvuII CAG'CTG
Cuts at: 0 546 966
Size: 546 420
SanDI GG'GwC_CC
Cuts at: 0 150 194 966
Size: 150 44 772
Fragments arranged by size:
772 150 44
SexAI A'CCwGG_T
Cuts at: 0 407 966
Size: 407 559
SfcI C'TryA_G
Cuts at: 0 720 966
Size: 720 246
SspI AAT'ATT
Cuts at: 0 102 966
Size: 102 864
StyI C'CwwG_G
Cuts at: 0 741 966
Size: 741 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 348 816 903 966
Size: 19 329 468 87 63
Fragments arranged by size:
468 329 87 63 19
Tth111I GACn'n_nGTC
Cuts at: 0 792 966
Size: 792 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 966
Size: 636 330
Enzymes that do cut:
AccI AfeI AhdI AleI AvrII BanII BbvCI BmrI
BpmI Bpu10I BsaI BseYI BsiHKAI BsmI Bsp1286I BsrDI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MslI MspA1I NdeI PfoI PpuMI PvuII SanDI SexAI
SfcI SspI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI XbaI XhoI XmaI XmnI ZraI