Restriction map for
pF1KB5855
(Linear) (Six-base) MAPSORT of: KIBB5855.seq Check: 1264 from: 1 to: 1377
KIBB5855.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
Acc65I G'GTAC_C
Cuts at: 0 1093 1377
Size: 1093 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 187 538 778 1377
Size: 74 113 351 240 599
Fragments arranged by size:
599 351 240 113 74
AfeI AGC'GCT
Cuts at: 0 1264 1377
Size: 1264 113
AleI CACnn'nnGTG
Cuts at: 0 196 944 1377
Size: 196 748 433
Fragments arranged by size:
748 433 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 821 1377
Size: 352 469 556
Fragments arranged by size:
556 469 352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1269 1301 1377
Size: 1269 32 76
Fragments arranged by size:
1269 76 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 646 1113 1377
Size: 300 346 467 264
Fragments arranged by size:
467 346 300 264
ApaI G_GGCC'C
Cuts at: 0 1133 1226 1377
Size: 1133 93 151
Fragments arranged by size:
1133 151 93
ApoI r'AATT_y
Cuts at: 0 368 1377
Size: 368 1009
AvaI C'yCGr_G
Cuts at: 0 257 1241 1307 1377
Size: 257 984 66 70
Fragments arranged by size:
984 257 70 66
BamHI G'GATC_C
Cuts at: 0 847 1377
Size: 847 530
BanI G'GyrC_C
Cuts at: 0 231 1058 1093 1377
Size: 231 827 35 284
Fragments arranged by size:
827 284 231 35
BanII G_rGCy'C
Cuts at: 0 611 1133 1145 1226 1377
Size: 611 522 12 81 151
Fragments arranged by size:
611 522 151 81 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 230 1377
Size: 230 1147
BglII A'GATC_T
Cuts at: 0 131 239 1377
Size: 131 108 1138
Fragments arranged by size:
1138 131 108
BlpI GC'TnA_GC
Cuts at: 0 767 1377
Size: 767 610
Bme1580I G_kGCm'C
Cuts at: 0 331 1133 1226 1377
Size: 331 802 93 151
Fragments arranged by size:
802 331 151 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 674 1019 1377
Size: 48 626 345 358
Fragments arranged by size:
626 358 345 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 628 1377
Size: 628 749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1120 1377
Size: 781 339 257
Fragments arranged by size:
781 339 257
BsaAI yAC'GTr
Cuts at: 0 309 1377
Size: 309 1068
BsaBI GATnn'nnATC
Cuts at: 0 136 244 1377
Size: 136 108 1133
Fragments arranged by size:
1133 136 108
BsaHI Gr'CG_yC
Cuts at: 0 7 1377
Size: 7 1370
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 908 1377
Size: 46 862 469
Fragments arranged by size:
862 469 46
BseYI C'CCAG_C
Cuts at: 0 210 300 319 324 859 935 1256 1377
Size: 210 90 19 5 535 76 321 121
Fragments arranged by size:
535 321 210 121 90 76 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 621 704 1377
Size: 607 14 83 673
Fragments arranged by size:
673 607 83 14
Bsp1286I G_dGCh'C
Cuts at: 0 331 611 1133 1145 1226 1377
Size: 331 280 522 12 81 151
Fragments arranged by size:
522 331 280 151 81 12
BsrBI CCG'CTC
Cuts at: 0 741 1377
Size: 741 636
BsrFI r'CCGG_y
Cuts at: 0 1325 1377
Size: 1325 52
BssSI C'ACGA_G
Cuts at: 0 613 1377
Size: 613 764
BstYI r'GATC_y
Cuts at: 0 131 239 278 847 1011 1377
Size: 131 108 39 569 164 366
Fragments arranged by size:
569 366 164 131 108 39
Bsu36I CC'TnA_GG
Cuts at: 0 1124 1377
Size: 1124 253
BtgI C'CryG_G
Cuts at: 0 124 662 1145 1377
Size: 124 538 483 232
Fragments arranged by size:
538 483 232 124
BtsI GCAGTG_nn'
Cuts at: 0 155 329 471 477 595 1377
Size: 155 174 142 6 118 782
Fragments arranged by size:
782 174 155 142 118 6
EaeI y'GGCC_r
Cuts at: 0 311 396 1377
Size: 311 85 981
Fragments arranged by size:
981 311 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 187 538 778 1377
Size: 74 113 351 240 599
Fragments arranged by size:
599 351 240 113 74
EcoO109I rG'GnC_Cy
Cuts at: 0 42 207 1033 1129 1130 1186 1204 1222
Size: 42 165 826 96 1 56 18 18
Cuts at: 1222 1223 1377
Size: 1 154
Fragments arranged by size:
826 165 154 96 56 42 18 18
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 1377
Size: 456 921
HaeII r_GCGC'y
Cuts at: 0 1266 1377
Size: 1266 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 770 802 1377
Size: 613 32 125 32 575
Fragments arranged by size:
613 575 125 32 32
KpnI G_GTAC'C
Cuts at: 0 1097 1377
Size: 1097 280
MscI TGG'CCA
Cuts at: 0 313 398 1377
Size: 313 85 979
Fragments arranged by size:
979 313 85
MslI CAynn'nnrTG
Cuts at: 0 196 334 944 1377
Size: 196 138 610 433
Fragments arranged by size:
610 433 196 138
MspA1I CmG'CkG
Cuts at: 0 22 829 1214 1377
Size: 22 807 385 163
Fragments arranged by size:
807 385 163 22
NcoI C'CATG_G
Cuts at: 0 124 662 1145 1377
Size: 124 538 483 232
Fragments arranged by size:
538 483 232 124
NdeI CA'TA_TG
Cuts at: 0 943 1377
Size: 943 434
NspI r_CATG'y
Cuts at: 0 559 1377
Size: 559 818
PasI CC'CwG_GG
Cuts at: 0 1350 1377
Size: 1350 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 1377
Size: 504 873
PfoI T'CCnGG_A
Cuts at: 0 425 1377
Size: 425 952
PmlI CAC'GTG
Cuts at: 0 309 1377
Size: 309 1068
PpuMI rG'GwC_Cy
Cuts at: 0 42 207 1033 1186 1377
Size: 42 165 826 153 191
Fragments arranged by size:
826 191 165 153 42
PspOMI G'GGCC_C
Cuts at: 0 1129 1222 1377
Size: 1129 93 155
Fragments arranged by size:
1129 155 93
PstI C_TGCA'G
Cuts at: 0 21 27 162 1377
Size: 21 6 135 1215
Fragments arranged by size:
1215 135 21 6
PvuII CAG'CTG
Cuts at: 0 22 829 1214 1377
Size: 22 807 385 163
Fragments arranged by size:
807 385 163 22
SexAI A'CCwGG_T
Cuts at: 0 964 1377
Size: 964 413
SfcI C'TryA_G
Cuts at: 0 17 23 91 158 830 1377
Size: 17 6 68 67 672 547
Fragments arranged by size:
672 547 68 67 17 6
SmlI C'TyrA_G
Cuts at: 0 760 1135 1377
Size: 760 375 242
Fragments arranged by size:
760 375 242
SphI G_CATG'C
Cuts at: 0 559 1377
Size: 559 818
StuI AGG'CCT
Cuts at: 0 439 1377
Size: 439 938
StyI C'CwwG_G
Cuts at: 0 124 314 662 1145 1173 1377
Size: 124 190 348 483 28 204
Fragments arranged by size:
483 348 204 190 124 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 1076 1120 1377
Size: 873 203 44 257
Fragments arranged by size:
873 257 203 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 132 164 881 913 1377
Size: 132 32 717 32 464
Fragments arranged by size:
717 464 132 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 40 966 1377
Size: 40 926 411
Fragments arranged by size:
926 411 40
XbaI T'CTAG_A
Cuts at: 0 81 1377
Size: 81 1296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 1377
Size: 321 1056
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BciVI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaAI BsaBI BsaHI BseRI
BseYI BsgI Bsp1286I BsrBI BsrFI BssSI BstYI Bsu36I
BtgI BtsI EaeI Eco57MI EcoO109I FalI HaeII Hin4I
KpnI MscI MslI MspA1I NcoI NdeI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SexAI
SfcI SmlI SphI StuI StyI TaqII TstI Tth111I
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TatI XhoI XmaI
XmnI ZraI