Restriction map for
pF1KB5863
(Linear) (Six-base) MAPSORT of: KIBB5863.seq Check: 4327 from: 1 to: 1383
KIBB5863.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 1383
Size: 666 717
AccI GT'mk_AC
Cuts at: 0 260 1383
Size: 260 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1383
Size: 336 1047
AlwNI CAG_nnn'CTG
Cuts at: 0 108 374 671 1383
Size: 108 266 297 712
Fragments arranged by size:
712 297 266 108
ApaI G_GGCC'C
Cuts at: 0 1214 1383
Size: 1214 169
ApoI r'AATT_y
Cuts at: 0 44 212 1383
Size: 44 168 1171
Fragments arranged by size:
1171 168 44
AvaI C'yCGr_G
Cuts at: 0 1171 1383
Size: 1171 212
BamHI G'GATC_C
Cuts at: 0 113 1331 1383
Size: 113 1218 52
Fragments arranged by size:
1218 113 52
BanI G'GyrC_C
Cuts at: 0 1234 1377 1383
Size: 1234 143 6
Fragments arranged by size:
1234 143 6
BanII G_rGCy'C
Cuts at: 0 149 989 1214 1283 1383
Size: 149 840 225 69 100
Fragments arranged by size:
840 225 149 100 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 611 620 846 1383
Size: 611 9 226 537
Fragments arranged by size:
611 537 226 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 315 349 1383
Size: 315 34 1034
Fragments arranged by size:
1034 315 34
BglI GCCn_nnn'nGGC
Cuts at: 0 371 715 1383
Size: 371 344 668
Fragments arranged by size:
668 371 344
BglII A'GATC_T
Cuts at: 0 185 1383
Size: 185 1198
Bme1580I G_kGCm'C
Cuts at: 0 99 323 950 1214 1343 1380 1383
Size: 99 224 627 264 129 37 3
Fragments arranged by size:
627 264 224 129 99 37 3
BmgBI CAC'GTC
Cuts at: 0 258 1383
Size: 258 1125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 160 184 1383
Size: 66 94 24 1199
Fragments arranged by size:
1199 94 66 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 1383
Size: 1000 383
BsaAI yAC'GTr
Cuts at: 0 329 1383
Size: 329 1054
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 45 75 157 187 283 313 1383
Size: 45 30 82 30 96 30 1070
Fragments arranged by size:
1070 96 82 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 157 196 214 472 1383
Size: 53 104 39 18 258 911
Fragments arranged by size:
911 258 104 53 39 18
BseYI C'CCAG_C
Cuts at: 0 374 501 815 917 1022 1262 1373 1383
Size: 374 127 314 102 105 240 111 10
Fragments arranged by size:
374 314 240 127 111 105 102 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 892 1137 1383
Size: 423 469 245 246
Fragments arranged by size:
469 423 246 245
BsiHKAI G_wGCw'C
Cuts at: 0 149 296 725 883 989 1383
Size: 149 147 429 158 106 394
Fragments arranged by size:
429 394 158 149 147 106
Bsp1286I G_dGCh'C
Cuts at: 0 99 149 296 323 725 883 950 989
Size: 99 50 147 27 402 158 67 39
Cuts at: 989 1214 1283 1343 1380 1383
Size: 225 69 60 37 3
Fragments arranged by size:
402 225 158 147 99 69 67 60
50 39 37 27 3
BspHI T'CATG_A
Cuts at: 0 1127 1383
Size: 1127 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 815 1383
Size: 666 149 568
Fragments arranged by size:
666 568 149
BsrBI CCG'CTC
Cuts at: 0 168 1383
Size: 168 1215
BsrFI r'CCGG_y
Cuts at: 0 997 1178 1383
Size: 997 181 205
Fragments arranged by size:
997 205 181
BssSI C'ACGA_G
Cuts at: 0 1012 1383
Size: 1012 371
BstEII G'GTnAC_C
Cuts at: 0 265 1383
Size: 265 1118
BstYI r'GATC_y
Cuts at: 0 113 185 624 1331 1383
Size: 113 72 439 707 52
Fragments arranged by size:
707 439 113 72 52
BtgI C'CryG_G
Cuts at: 0 628 1097 1214 1230 1335 1383
Size: 628 469 117 16 105 48
Fragments arranged by size:
628 469 117 105 48 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 882 1383
Size: 882 501
BtsI GCAGTG_nn'
Cuts at: 0 526 539 725 756 901 1383
Size: 526 13 186 31 145 482
Fragments arranged by size:
526 482 186 145 31 13
DraIII CAC_nnn'GTG
Cuts at: 0 105 1383
Size: 105 1278
EaeI y'GGCC_r
Cuts at: 0 32 999 1383
Size: 32 967 384
Fragments arranged by size:
967 384 32
EarI CTCTTCn'nnn_
Cuts at: 0 1363 1383
Size: 1363 20
EcoICRI GAG'CTC
Cuts at: 0 147 987 1383
Size: 147 840 396
Fragments arranged by size:
840 396 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 160 184 336 1383
Size: 66 94 24 152 1047
Fragments arranged by size:
1047 152 94 66 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1383
Size: 457 926
EcoO109I rG'GnC_Cy
Cuts at: 0 554 1210 1383
Size: 554 656 173
Fragments arranged by size:
656 554 173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1383
Size: 870 513
FseI GG_CCGG'CC
Cuts at: 0 1001 1383
Size: 1001 382
HaeII r_GCGC'y
Cuts at: 0 477 816 1383
Size: 477 339 567
Fragments arranged by size:
567 477 339
HincII GTy'rAC
Cuts at: 0 261 1383
Size: 261 1122
MfeI C'AATT_G
Cuts at: 0 414 1383
Size: 414 969
MscI TGG'CCA
Cuts at: 0 34 1383
Size: 34 1349
MspA1I CmG'CkG
Cuts at: 0 356 371 630 751 968 1383
Size: 356 15 259 121 217 415
Fragments arranged by size:
415 356 259 217 121 15
NaeI GCC'GGC
Cuts at: 0 999 1383
Size: 999 384
NcoI C'CATG_G
Cuts at: 0 1214 1335 1383
Size: 1214 121 48
Fragments arranged by size:
1214 121 48
NgoMIV G'CCGG_C
Cuts at: 0 997 1383
Size: 997 386
NspI r_CATG'y
Cuts at: 0 1155 1383
Size: 1155 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 276 762 1383
Size: 225 51 486 621
Fragments arranged by size:
621 486 225 51
PfoI T'CCnGG_A
Cuts at: 0 137 959 1383
Size: 137 822 424
Fragments arranged by size:
822 424 137
PmlI CAC'GTG
Cuts at: 0 329 1383
Size: 329 1054
PspOMI G'GGCC_C
Cuts at: 0 1210 1383
Size: 1210 173
PstI C_TGCA'G
Cuts at: 0 521 1383
Size: 521 862
PvuII CAG'CTG
Cuts at: 0 356 1383
Size: 356 1027
SacI G_AGCT'C
Cuts at: 0 149 989 1383
Size: 149 840 394
Fragments arranged by size:
840 394 149
SacII CC_GC'GG
Cuts at: 0 631 1383
Size: 631 752
SalI G'TCGA_C
Cuts at: 0 259 1383
Size: 259 1124
SapI GCTCTTCn'nnn_
Cuts at: 0 1363 1383
Size: 1363 20
SexAI A'CCwGG_T
Cuts at: 0 101 1237 1383
Size: 101 1136 146
Fragments arranged by size:
1136 146 101
SfcI C'TryA_G
Cuts at: 0 477 517 870 1383
Size: 477 40 353 513
Fragments arranged by size:
513 477 353 40
StyI C'CwwG_G
Cuts at: 0 206 287 1214 1335 1383
Size: 206 81 927 121 48
Fragments arranged by size:
927 206 121 81 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 729 1383
Size: 729 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 157 189 283 315 1383
Size: 45 32 80 32 94 32 1068
Fragments arranged by size:
1068 94 80 45 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 1383
Size: 135 1248
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BcgI BglI BglII Bme1580I BmgBI
BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI HaeII HincII MfeI MscI
MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI
PspOMI PstI PvuII SacI SacII SalI SapI SexAI
SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BlpI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FspI FspAI Hin4I HindIII
HpaI KasI KpnI MluI MmeI MslI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI