Restriction map for
pF1KB5864
(Linear) (Six-base) MAPSORT of: KIBB5864.seq Check: 9984 from: 1 to: 1473
KIBB5864.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 116 1473
Size: 116 1357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1228 1473
Size: 749 479 245
Fragments arranged by size:
749 479 245
AleI CACnn'nnGTG
Cuts at: 0 1409 1473
Size: 1409 64
AlwNI CAG_nnn'CTG
Cuts at: 0 36 45 1473
Size: 36 9 1428
Fragments arranged by size:
1428 36 9
ApaI G_GGCC'C
Cuts at: 0 72 1473
Size: 72 1401
ApoI r'AATT_y
Cuts at: 0 1131 1473
Size: 1131 342
AvaI C'yCGr_G
Cuts at: 0 4 1285 1473
Size: 4 1281 188
Fragments arranged by size:
1281 188 4
BamHI G'GATC_C
Cuts at: 0 428 1473
Size: 428 1045
BanII G_rGCy'C
Cuts at: 0 56 72 293 1473
Size: 56 16 221 1180
Fragments arranged by size:
1180 221 56 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 519 1473
Size: 519 954
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 74 1473
Size: 40 34 1399
Fragments arranged by size:
1399 40 34
BglI GCCn_nnn'nGGC
Cuts at: 0 67 1473
Size: 67 1406
BglII A'GATC_T
Cuts at: 0 782 1473
Size: 782 691
Bme1580I G_kGCm'C
Cuts at: 0 63 72 447 1473
Size: 63 9 375 1026
Fragments arranged by size:
1026 375 63 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 501 1473
Size: 501 972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1428 1473
Size: 94 1334 45
Fragments arranged by size:
1334 94 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 307 1394 1473
Size: 193 114 1087 79
Fragments arranged by size:
1087 193 114 79
BsaHI Gr'CG_yC
Cuts at: 0 452 1473
Size: 452 1021
BsaWI w'CCGG_w
Cuts at: 0 873 1473
Size: 873 600
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 116 146 630 660 1473
Size: 116 30 484 30 813
Fragments arranged by size:
813 484 116 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 363 1473
Size: 363 1110
BseYI C'CCAG_C
Cuts at: 0 233 1392 1473
Size: 233 1159 81
Fragments arranged by size:
1159 233 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1473
Size: 481 992
BsiHKAI G_wGCw'C
Cuts at: 0 56 80 1473
Size: 56 24 1393
Fragments arranged by size:
1393 56 24
BsmI GAATG_Cn'
Cuts at: 0 1452 1473
Size: 1452 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 633 1473
Size: 633 840
Bsp1286I G_dGCh'C
Cuts at: 0 56 63 72 80 293 447 1473
Size: 56 7 9 8 213 154 1026
Fragments arranged by size:
1026 213 154 56 9 8 7
BspHI T'CATG_A
Cuts at: 0 945 1473
Size: 945 528
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 1473
Size: 116 1357
BsrDI GCAATG_nn'
Cuts at: 0 1148 1439 1473
Size: 1148 291 34
Fragments arranged by size:
1148 291 34
BstYI r'GATC_y
Cuts at: 0 428 768 782 1053 1164 1473
Size: 428 340 14 271 111 309
Fragments arranged by size:
428 340 309 271 111 14
BtgI C'CryG_G
Cuts at: 0 63 241 447 1473
Size: 63 178 206 1026
Fragments arranged by size:
1026 206 178 63
BtsI GCAGTG_nn'
Cuts at: 0 176 1473
Size: 176 1297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 642 677 1473
Size: 642 35 796
Fragments arranged by size:
796 642 35
DraI TTT'AAA
Cuts at: 0 683 1136 1473
Size: 683 453 337
Fragments arranged by size:
683 453 337
EaeI y'GGCC_r
Cuts at: 0 47 957 1473
Size: 47 910 516
Fragments arranged by size:
910 516 47
EarI CTCTTCn'nnn_
Cuts at: 0 217 1207 1473
Size: 217 990 266
Fragments arranged by size:
990 266 217
EcoICRI GAG'CTC
Cuts at: 0 54 1473
Size: 54 1419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 749 1228 1428 1473
Size: 94 655 479 200 45
Fragments arranged by size:
655 479 200 94 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1473
Size: 1018 455
EcoO109I rG'GnC_Cy
Cuts at: 0 68 69 1473
Size: 68 1 1404
Fragments arranged by size:
1404 68 1
EcoRV GAT'ATC
Cuts at: 0 147 1026 1473
Size: 147 879 447
Fragments arranged by size:
879 447 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 1473
Size: 927 546
HaeII r_GCGC'y
Cuts at: 0 37 468 1473
Size: 37 431 1005
Fragments arranged by size:
1005 431 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 753 785 922 954 1473
Size: 116 32 605 32 137 32 519
Fragments arranged by size:
605 519 137 116 32 32 32
HincII GTy'rAC
Cuts at: 0 575 1473
Size: 575 898
HindIII A'AGCT_T
Cuts at: 0 904 1473
Size: 904 569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1473
Size: 1114 359
MslI CAynn'nnrTG
Cuts at: 0 1360 1409 1473
Size: 1360 49 64
Fragments arranged by size:
1360 64 49
MspA1I CmG'CkG
Cuts at: 0 42 86 1473
Size: 42 44 1387
Fragments arranged by size:
1387 44 42
NcoI C'CATG_G
Cuts at: 0 447 1473
Size: 447 1026
PasI CC'CwG_GG
Cuts at: 0 1001 1473
Size: 1001 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 966 1096 1473
Size: 966 130 377
Fragments arranged by size:
966 377 130
PfoI T'CCnGG_A
Cuts at: 0 431 1473
Size: 431 1042
PsiI TTA'TAA
Cuts at: 0 1120 1473
Size: 1120 353
PspOMI G'GGCC_C
Cuts at: 0 68 1473
Size: 68 1405
PstI C_TGCA'G
Cuts at: 0 41 1473
Size: 41 1432
PvuII CAG'CTG
Cuts at: 0 42 86 1473
Size: 42 44 1387
Fragments arranged by size:
1387 44 42
SacI G_AGCT'C
Cuts at: 0 56 1473
Size: 56 1417
SfcI C'TryA_G
Cuts at: 0 37 1473
Size: 37 1436
SmaI CCC'GGG
Cuts at: 0 6 1473
Size: 6 1467
StuI AGG'CCT
Cuts at: 0 257 1473
Size: 257 1216
StyI C'CwwG_G
Cuts at: 0 447 746 1473
Size: 447 299 727
Fragments arranged by size:
727 447 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1473
Size: 433 1040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1438 1470 1473
Size: 1230 32 176 32 3
Fragments arranged by size:
1230 176 32 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1320 1473
Size: 1047 273 153
Fragments arranged by size:
1047 273 153
XmaI C'CCGG_G
Cuts at: 0 4 1473
Size: 4 1469
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApoI AvaI BamHI
BanII BbsI BcgI BglI BglII Bme1580I BplI BpmI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BstYI BtgI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MmeI
MslI MspA1I NcoI PasI PflMI PfoI PsiI PspOMI
PstI PvuII SacI SfcI SmaI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsiEI
BsiWI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII
DrdI EagI EciI EcoRI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI