Restriction map for
pF1KB5865
(Linear) (Six-base) MAPSORT of: KIBB5865.seq Check: 8940 from: 1 to: 1383
KIBB5865.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1228 1383
Size: 1228 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 220 268 520 943 1383
Size: 124 96 48 252 423 440
Fragments arranged by size:
440 423 252 124 96 48
AleI CACnn'nnGTG
Cuts at: 0 976 1383
Size: 976 407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 1383
Size: 994 389
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 1383
Size: 1015 32 336
Fragments arranged by size:
1015 336 32
AlwNI CAG_nnn'CTG
Cuts at: 0 15 102 663 816 921 945 1200 1293
Size: 15 87 561 153 105 24 255 93
Cuts at: 1293 1344 1383
Size: 51 39
Fragments arranged by size:
561 255 153 105 93 87 51 39
24 15
ApaLI G'TGCA_C
Cuts at: 0 398 1383
Size: 398 985
ApoI r'AATT_y
Cuts at: 0 409 1383
Size: 409 974
AvaI C'yCGr_G
Cuts at: 0 928 1165 1383
Size: 928 237 218
Fragments arranged by size:
928 237 218
BamHI G'GATC_C
Cuts at: 0 262 1383
Size: 262 1121
BanII G_rGCy'C
Cuts at: 0 719 807 1354 1383
Size: 719 88 547 29
Fragments arranged by size:
719 547 88 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 452 673 1383
Size: 452 221 710
Fragments arranged by size:
710 452 221
BbvCI CC'TCA_GC
Cuts at: 0 464 1383
Size: 464 919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1072 1106 1156 1190 1383
Size: 1072 34 50 34 193
Fragments arranged by size:
1072 193 50 34 34
BclI T'GATC_A
Cuts at: 0 563 692 1383
Size: 563 129 691
Fragments arranged by size:
691 563 129
BlpI GC'TnA_GC
Cuts at: 0 1049 1383
Size: 1049 334
Bme1580I G_kGCm'C
Cuts at: 0 402 973 1383
Size: 402 571 410
Fragments arranged by size:
571 410 402
BmrI ACTGGGnnnn_n'
Cuts at: 0 299 1210 1383
Size: 299 911 173
Fragments arranged by size:
911 299 173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 573 1324 1383
Size: 220 353 751 59
Fragments arranged by size:
751 353 220 59
Bpu10I CC'TnA_GC
Cuts at: 0 464 1383
Size: 464 919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1383
Size: 376 1007
BsaAI yAC'GTr
Cuts at: 0 270 1383
Size: 270 1113
BsaHI Gr'CG_yC
Cuts at: 0 1205 1383
Size: 1205 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1015 1045 1383
Size: 1015 30 338
Fragments arranged by size:
1015 338 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 190 193 1054 1057 1383
Size: 26 164 3 861 3 326
Fragments arranged by size:
861 326 164 26 3 3
BseYI C'CCAG_C
Cuts at: 0 747 908 1247 1347 1383
Size: 747 161 339 100 36
Fragments arranged by size:
747 339 161 100 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1383
Size: 979 404
BsiHKAI G_wGCw'C
Cuts at: 0 402 883 983 1383
Size: 402 481 100 400
Fragments arranged by size:
481 402 400 100
BsiWI C'GTAC_G
Cuts at: 0 270 1383
Size: 270 1113
Bsp1286I G_dGCh'C
Cuts at: 0 402 719 807 883 973 983 1354 1383
Size: 402 317 88 76 90 10 371 29
Fragments arranged by size:
402 371 317 90 88 76 29 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 930 1228 1383
Size: 671 259 298 155
Fragments arranged by size:
671 298 259 155
BsrBI CCG'CTC
Cuts at: 0 846 1383
Size: 846 537
BssSI C'ACGA_G
Cuts at: 0 721 1383
Size: 721 662
BstAPI GCAn_nnn'nTGC
Cuts at: 0 663 816 1383
Size: 663 153 567
Fragments arranged by size:
663 567 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1118 1383
Size: 1118 265
BstYI r'GATC_y
Cuts at: 0 262 387 1383
Size: 262 125 996
Fragments arranged by size:
996 262 125
Bsu36I CC'TnA_GG
Cuts at: 0 946 1383
Size: 946 437
BtgI C'CryG_G
Cuts at: 0 833 1383
Size: 833 550
BtsI GCAGTG_nn'
Cuts at: 0 848 1000 1383
Size: 848 152 383
Fragments arranged by size:
848 383 152
EaeI y'GGCC_r
Cuts at: 0 65 830 1265 1295 1383
Size: 65 765 435 30 88
Fragments arranged by size:
765 435 88 65 30
EarI CTCTTCn'nnn_
Cuts at: 0 127 484 593 1383
Size: 127 357 109 790
Fragments arranged by size:
790 357 127 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 472 1383
Size: 472 911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 220 268 520 573 943 1324 1383
Size: 124 96 48 252 53 370 381 59
Fragments arranged by size:
381 370 252 124 96 59 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 724 958 1383
Size: 724 234 425
Fragments arranged by size:
724 425 234
EcoO109I rG'GnC_Cy
Cuts at: 0 231 660 864 942 950 1242 1383
Size: 231 429 204 78 8 292 141
Fragments arranged by size:
429 292 231 204 141 78 8
EcoRV GAT'ATC
Cuts at: 0 360 1383
Size: 360 1023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 525 1383
Size: 495 30 858
Fragments arranged by size:
858 495 30
FspI TGC'GCA
Cuts at: 0 656 1383
Size: 656 727
HaeII r_GCGC'y
Cuts at: 0 755 1162 1383
Size: 755 407 221
Fragments arranged by size:
755 407 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1383
Size: 21 32 1330
Fragments arranged by size:
1330 32 21
HincII GTy'rAC
Cuts at: 0 1359 1383
Size: 1359 24
MscI TGG'CCA
Cuts at: 0 832 1267 1297 1383
Size: 832 435 30 86
Fragments arranged by size:
832 435 86 30
MslI CAynn'nnrTG
Cuts at: 0 976 1383
Size: 976 407
MspA1I CmG'CkG
Cuts at: 0 912 1383
Size: 912 471
NspI r_CATG'y
Cuts at: 0 48 546 1383
Size: 48 498 837
Fragments arranged by size:
837 498 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 645 1155 1293 1383
Size: 645 510 138 90
Fragments arranged by size:
645 510 138 90
PfoI T'CCnGG_A
Cuts at: 0 197 1337 1383
Size: 197 1140 46
Fragments arranged by size:
1140 197 46
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1015 1047 1383
Size: 310 32 673 32 336
Fragments arranged by size:
673 336 310 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 660 864 942 950 1242 1383
Size: 660 204 78 8 292 141
Fragments arranged by size:
660 292 204 141 78 8
PstI C_TGCA'G
Cuts at: 0 261 812 941 1085 1196 1277 1313 1383
Size: 261 551 129 144 111 81 36 70
Fragments arranged by size:
551 261 144 129 111 81 70 36
SapI GCTCTTCn'nnn_
Cuts at: 0 127 484 1383
Size: 127 357 899
Fragments arranged by size:
899 357 127
SbfI CC_TGCA'GG
Cuts at: 0 261 1277 1383
Size: 261 1016 106
Fragments arranged by size:
1016 261 106
SexAI A'CCwGG_T
Cuts at: 0 1013 1286 1383
Size: 1013 273 97
Fragments arranged by size:
1013 273 97
SfcI C'TryA_G
Cuts at: 0 257 808 937 1081 1192 1273 1309 1383
Size: 257 551 129 144 111 81 36 74
Fragments arranged by size:
551 257 144 129 111 81 74 36
SmlI C'TyrA_G
Cuts at: 0 391 1383
Size: 391 992
SphI G_CATG'C
Cuts at: 0 48 1383
Size: 48 1335
SspI AAT'ATT
Cuts at: 0 631 1383
Size: 631 752
StuI AGG'CCT
Cuts at: 0 1115 1383
Size: 1115 268
StyI C'CwwG_G
Cuts at: 0 773 1383
Size: 773 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1190 1222 1383
Size: 1190 32 161
Fragments arranged by size:
1190 161 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 1383
Size: 1015 368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 780 1383
Size: 591 189 603
Fragments arranged by size:
603 591 189
XmnI GAAnn'nnTTC
Cuts at: 0 1030 1383
Size: 1030 353
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaLI ApoI
AvaI BamHI BanII BbsI BbvCI BcgI BclI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspMI BsrBI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
HaeII Hin4I HincII MscI MslI MspA1I NspI PflMI
PfoI PpiI PpuMI PstI SapI SbfI SexAI SfcI
SmlI SphI SspI StuI StyI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BciVI BfrBI BglI BglII BmgBI BmtI BplI
BsaI BsaBI BsaWI BsiEI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TaqII TatI XbaI
XhoI XmaI ZraI