(Linear) (Six-base) MAPSORT of: KIBB5866.seq  Check: 9742  from: 1  to: 1473



KIBB5866.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:      0     803    1473 
   Size:        803     670
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105    1197    1473 
   Size:        105    1092     276

  Fragments arranged by size: 

                1092     276     105
 
AflIII A'CryG_T

Cuts at:      0     440    1473 
   Size:        440    1033
 
BamHI G'GATC_C

Cuts at:      0     888    1473 
   Size:        888     585
 
BanII G_rGCy'C

Cuts at:      0     219    1473 
   Size:        219    1254
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     277    1286    1473 
   Size:        277    1009     187

  Fragments arranged by size: 

                1009     277     187
 
BglII A'GATC_T

Cuts at:      0    1106    1473 
   Size:       1106     367
 
Bme1580I G_kGCm'C

Cuts at:      0     129    1473 
   Size:        129    1344
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1350    1473 
   Size:       1350     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     534    1473 
   Size:        534     939
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1473 
   Size:        296    1177
 
BsaAI yAC'GTr

Cuts at:      0    1220    1473 
   Size:       1220     253
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1037    1067    1473 
   Size:       1037      30     406

  Fragments arranged by size: 

                1037     406      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     411     507    1473 
   Size:        124     287      96     966

  Fragments arranged by size: 

                 966     287     124      96
 
BseYI C'CCAG_C

Cuts at:      0     169    1473 
   Size:        169    1304
 
Bsp1286I G_dGCh'C

Cuts at:      0     129     219    1473 
   Size:        129      90    1254

  Fragments arranged by size: 

                1254     129      90
 
BsrFI r'CCGG_y

Cuts at:      0    1135    1473 
   Size:       1135     338
 
BsrGI T'GTAC_A

Cuts at:      0    1080    1473 
   Size:       1080     393
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     677    1473 
   Size:        677     796
 
BstYI r'GATC_y

Cuts at:      0     888    1106    1254    1473 
   Size:        888     218     148     219

  Fragments arranged by size: 

                 888     219     218     148
 
BstZ17I GTA'TAC

Cuts at:      0     804    1473 
   Size:        804     669
 
Bsu36I CC'TnA_GG

Cuts at:      0     175    1473 
   Size:        175    1298
 
BtgI C'CryG_G

Cuts at:      0     129    1473 
   Size:        129    1344
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     784    1473 
   Size:        784     689
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     155     429    1473 
   Size:        148       7     274    1044

  Fragments arranged by size: 

                1044     274     148       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     296    1197    1473 
   Size:        105     191     901     276

  Fragments arranged by size: 

                 901     276     191     105
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1006    1454    1473 
   Size:       1006     448      19

  Fragments arranged by size: 

                1006     448      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1144    1176    1473 
   Size:       1144      32     297

  Fragments arranged by size: 

                1144     297      32
 
HincII GTy'rAC

Cuts at:      0      99     870    1247    1473 
   Size:         99     771     377     226

  Fragments arranged by size: 

                 771     377     226      99
 
HpaI GTT'AAC

Cuts at:      0    1247    1473 
   Size:       1247     226
 
MspA1I CmG'CkG

Cuts at:      0     235    1167    1473 
   Size:        235     932     306

  Fragments arranged by size: 

                 932     306     235
 
NspI r_CATG'y

Cuts at:      0     444    1209    1473 
   Size:        444     765     264

  Fragments arranged by size: 

                 765     444     264
 
PciI A'CATG_T

Cuts at:      0     440    1473 
   Size:        440    1033
 
PfoI T'CCnGG_A

Cuts at:      0     602    1473 
   Size:        602     871
 
PpuMI rG'GwC_Cy

Cuts at:      0    1006    1473 
   Size:       1006     467
 
PvuII CAG'CTG

Cuts at:      0     235    1167    1473 
   Size:        235     932     306

  Fragments arranged by size: 

                 932     306     235
 
SanDI GG'GwC_CC

Cuts at:      0    1006    1473 
   Size:       1006     467
 
ScaI AGT'ACT

Cuts at:      0     514    1473 
   Size:        514     959
 
SexAI A'CCwGG_T

Cuts at:      0     954    1473 
   Size:        954     519
 
SfcI C'TryA_G

Cuts at:      0     320    1473 
   Size:        320    1153
 
SspI AAT'ATT

Cuts at:      0     664     696     964    1473 
   Size:        664      32     268     509

  Fragments arranged by size: 

                 664     509     268      32
 
StuI AGG'CCT

Cuts at:      0     180    1473 
   Size:        180    1293
 
StyI C'CwwG_G

Cuts at:      0      90    1473 
   Size:         90    1383
 
TatI w'GTAC_w

Cuts at:      0     437     512    1080    1352    1473 
   Size:        437      75     568     272     121

  Fragments arranged by size: 

                 568     437     272     121      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     729     761     824     856    1473 
   Size:        729      32      63      32     617

  Fragments arranged by size: 

                 729     617      63      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     962    1473 
   Size:        962     511
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    BamHI    BanII     BbsI    BglII Bme1580I
     BmrI     BplI     BpmI    BsaAI    BsaXI    BseRI    BseYI Bsp1286I
    BsrFI    BsrGI   BstAPI    BstYI  BstZ17I   Bsu36I     BtgI     DrdI
     EarI  Eco57MI EcoO109I    Hin4I   HincII     HpaI   MspA1I     NspI
     PciI     PfoI    PpuMI    PvuII    SanDI     ScaI    SexAI     SfcI
     SspI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI   BssHII    BssSI    BstBI   BstEII    BstXI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI