Restriction map for
pF1KB5868
(Linear) (Six-base) MAPSORT of: KIBB5868.seq Check: 2719 from: 1 to: 1479
KIBB5868.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 638 1181 1479
Size: 638 543 298
Fragments arranged by size:
638 543 298
AflIII A'CryG_T
Cuts at: 0 506 991 1479
Size: 506 485 488
Fragments arranged by size:
506 488 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 615 1479
Size: 615 864
AlwNI CAG_nnn'CTG
Cuts at: 0 382 1479
Size: 382 1097
ApaLI G'TGCA_C
Cuts at: 0 528 1479
Size: 528 951
ApoI r'AATT_y
Cuts at: 0 943 1479
Size: 943 536
AvaI C'yCGr_G
Cuts at: 0 340 1479
Size: 340 1139
BamHI G'GATC_C
Cuts at: 0 105 583 1479
Size: 105 478 896
Fragments arranged by size:
896 478 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1479
Size: 884 595
BbvCI CC'TCA_GC
Cuts at: 0 251 437 1479
Size: 251 186 1042
Fragments arranged by size:
1042 251 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 548 1115 1149 1479
Size: 514 34 567 34 330
Fragments arranged by size:
567 514 330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 193 478 961 1479
Size: 193 285 483 518
Fragments arranged by size:
518 483 285 193
BlpI GC'TnA_GC
Cuts at: 0 794 853 1479
Size: 794 59 626
Fragments arranged by size:
794 626 59
Bme1580I G_kGCm'C
Cuts at: 0 532 604 961 1479
Size: 532 72 357 518
Fragments arranged by size:
532 518 357 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1132 1164 1479
Size: 1132 32 315
Fragments arranged by size:
1132 315 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 1479
Size: 220 1259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 1479
Size: 1414 65
Bpu10I CC'TnA_GC
Cuts at: 0 251 437 1479
Size: 251 186 1042
Fragments arranged by size:
1042 251 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 1479
Size: 885 594
BsaI GGTCTCn'nnnn_
Cuts at: 0 1009 1479
Size: 1009 470
BsaAI yAC'GTr
Cuts at: 0 74 1479
Size: 74 1405
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 645 675 1479
Size: 645 30 804
Fragments arranged by size:
804 645 30
BseYI C'CCAG_C
Cuts at: 0 426 1479
Size: 426 1053
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 735 1479
Size: 497 238 744
Fragments arranged by size:
744 497 238
BsiHKAI G_wGCw'C
Cuts at: 0 532 795 1352 1479
Size: 532 263 557 127
Fragments arranged by size:
557 532 263 127
BsmI GAATG_Cn'
Cuts at: 0 443 895 1033 1479
Size: 443 452 138 446
Fragments arranged by size:
452 446 443 138
Bsp1286I G_dGCh'C
Cuts at: 0 532 604 795 961 1352 1479
Size: 532 72 191 166 391 127
Fragments arranged by size:
532 391 191 166 127 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 760 1479
Size: 760 719
BsrDI GCAATG_nn'
Cuts at: 0 88 127 1346 1479
Size: 88 39 1219 133
Fragments arranged by size:
1219 133 88 39
BssSI C'ACGA_G
Cuts at: 0 1351 1479
Size: 1351 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1479
Size: 318 1161
BstYI r'GATC_y
Cuts at: 0 105 583 1098 1479
Size: 105 478 515 381
Fragments arranged by size:
515 478 381 105
Bsu36I CC'TnA_GG
Cuts at: 0 282 1479
Size: 282 1197
BtsI GCAGTG_nn'
Cuts at: 0 530 637 805 864 1479
Size: 530 107 168 59 615
Fragments arranged by size:
615 530 168 107 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 282 317 1442 1477 1479
Size: 282 35 1125 35 2
Fragments arranged by size:
1125 282 35 35 2
DraIII CAC_nnn'GTG
Cuts at: 0 507 1479
Size: 507 972
DrdI GACnn_nn'nnGTC
Cuts at: 0 1325 1479
Size: 1325 154
EaeI y'GGCC_r
Cuts at: 0 356 396 1479
Size: 356 40 1083
Fragments arranged by size:
1083 356 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 435 1479
Size: 435 1044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 1479
Size: 1414 65
EcoO109I rG'GnC_Cy
Cuts at: 0 379 1421 1479
Size: 379 1042 58
Fragments arranged by size:
1042 379 58
EcoRV GAT'ATC
Cuts at: 0 613 1479
Size: 613 866
HaeII r_GCGC'y
Cuts at: 0 40 1479
Size: 40 1439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1367 1399 1479
Size: 1367 32 80
Fragments arranged by size:
1367 80 32
HincII GTy'rAC
Cuts at: 0 186 1479
Size: 186 1293
MfeI C'AATT_G
Cuts at: 0 730 1479
Size: 730 749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1030 1479
Size: 476 554 449
Fragments arranged by size:
554 476 449
MscI TGG'CCA
Cuts at: 0 358 1479
Size: 358 1121
MslI CAynn'nnrTG
Cuts at: 0 597 954 1479
Size: 597 357 525
Fragments arranged by size:
597 525 357
MspA1I CmG'CkG
Cuts at: 0 430 1479
Size: 430 1049
NspI r_CATG'y
Cuts at: 0 596 995 1479
Size: 596 399 484
Fragments arranged by size:
596 484 399
PasI CC'CwG_GG
Cuts at: 0 963 1479
Size: 963 516
PciI A'CATG_T
Cuts at: 0 991 1479
Size: 991 488
PfoI T'CCnGG_A
Cuts at: 0 606 1479
Size: 606 873
PpuMI rG'GwC_Cy
Cuts at: 0 1421 1479
Size: 1421 58
PstI C_TGCA'G
Cuts at: 0 372 537 1479
Size: 372 165 942
Fragments arranged by size:
942 372 165
PvuII CAG'CTG
Cuts at: 0 430 1479
Size: 430 1049
SfcI C'TryA_G
Cuts at: 0 368 533 549 1479
Size: 368 165 16 930
Fragments arranged by size:
930 368 165 16
SmaI CCC'GGG
Cuts at: 0 342 1479
Size: 342 1137
SmlI C'TyrA_G
Cuts at: 0 900 1479
Size: 900 579
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 959 991 1030 1062 1479
Size: 959 32 39 32 417
Fragments arranged by size:
959 417 39 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 148 1479
Size: 148 1331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 718 1479
Size: 718 761
XmaI C'CCGG_G
Cuts at: 0 340 1479
Size: 340 1139
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaLI ApoI AvaI BamHI
BbsI BbvCI BcgI BciVI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstXI BstYI
Bsu36I BtsI CspCI DraIII DrdI EaeI EciI Eco57MI
EcoO109I EcoRV HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NspI PasI PciI PfoI PpuMI PstI
PvuII SfcI SmaI SmlI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BanII BbeI BclI BfrBI BglI BglII
BmgBI BmtI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI DraI EagI
EarI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
XbaI XhoI XmnI ZraI