(Linear) (Six-base) MAPSORT of: KIBB5878.seq  Check: 9902  from: 1  to: 1485



KIBB5878.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877    1485 
   Size:        877     608
 
AflIII A'CryG_T

Cuts at:      0      78    1485 
   Size:         78    1407
 
ApoI r'AATT_y

Cuts at:      0      18    1144    1469    1485 
   Size:         18    1126     325      16

  Fragments arranged by size: 

                1126     325      18      16
 
AseI AT'TA_AT

Cuts at:      0    1171    1319    1485 
   Size:       1171     148     166

  Fragments arranged by size: 

                1171     166     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     536    1485 
   Size:        536     949
 
BbvCI CC'TCA_GC

Cuts at:      0    1151    1485 
   Size:       1151     334
 
BglII A'GATC_T

Cuts at:      0     665    1485 
   Size:        665     820
 
BmtI G_CTAG'C

Cuts at:      0    1141    1485 
   Size:       1141     344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     783    1485 
   Size:        238     545     702

  Fragments arranged by size: 

                 702     545     238
 
Bpu10I CC'TnA_GC

Cuts at:      0    1151    1485 
   Size:       1151     334
 
BsaBI GATnn'nnATC

Cuts at:      0    1421    1485 
   Size:       1421      64
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     796     889    1129    1485 
   Size:        796      93     240     356

  Fragments arranged by size: 

                 796     356     240      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148    1485 
   Size:        148    1337
 
BsmI GAATG_Cn'

Cuts at:      0     525     720     903    1182    1485 
   Size:        525     195     183     279     303

  Fragments arranged by size: 

                 525     303     279     195     183
 
BspHI T'CATG_A

Cuts at:      0     641    1485 
   Size:        641     844
 
BsrDI GCAATG_nn'

Cuts at:      0     725    1485 
   Size:        725     760
 
BstYI r'GATC_y

Cuts at:      0     665    1485 
   Size:        665     820
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1254    1289    1485 
   Size:       1254      35     196

  Fragments arranged by size: 

                1254     196      35
 
DraI TTT'AAA

Cuts at:      0    1404    1485 
   Size:       1404      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     203     509     731    1106    1232    1277    1430    1472 
   Size:        203     306     222     375     126      45     153      42

Cuts at:   1472    1485 
   Size:         13

  Fragments arranged by size: 

                 375     306     222     203     153     126      45      42
                  13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     783     877    1485 
   Size:        238     545      94     608

  Fragments arranged by size: 

                 608     545     238      94
 
HincII GTy'rAC

Cuts at:      0     102     905    1485 
   Size:        102     803     580

  Fragments arranged by size: 

                 803     580     102
 
HindIII A'AGCT_T

Cuts at:      0     611    1485 
   Size:        611     874
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     665    1485 
   Size:        179     486     820

  Fragments arranged by size: 

                 820     486     179
 
MspA1I CmG'CkG

Cuts at:      0     624    1203    1485 
   Size:        624     579     282

  Fragments arranged by size: 

                 624     579     282
 
NheI G'CTAG_C

Cuts at:      0    1137    1485 
   Size:       1137     348
 
NspI r_CATG'y

Cuts at:      0      82     828    1366    1485 
   Size:         82     746     538     119

  Fragments arranged by size: 

                 746     538     119      82
 
PciI A'CATG_T

Cuts at:      0      78    1485 
   Size:         78    1407
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     242     274    1485 
   Size:        242      32    1211

  Fragments arranged by size: 

                1211     242      32
 
PstI C_TGCA'G

Cuts at:      0     528    1485 
   Size:        528     957
 
PvuII CAG'CTG

Cuts at:      0     624    1203    1485 
   Size:        624     579     282

  Fragments arranged by size: 

                 624     579     282
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1232    1485 
   Size:       1232     253
 
ScaI AGT'ACT

Cuts at:      0     559    1485 
   Size:        559     926
 
SfcI C'TryA_G

Cuts at:      0      27     524     886    1093    1485 
   Size:         27     497     362     207     392

  Fragments arranged by size: 

                 497     392     362     207      27
 
SphI G_CATG'C

Cuts at:      0    1366    1485 
   Size:       1366     119
 
TatI w'GTAC_w

Cuts at:      0     557    1485 
   Size:        557     928
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483    1485 
   Size:        483    1002
 
XmnI GAAnn'nnTTC

Cuts at:      0     740    1485 
   Size:        740     745
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI     BbsI    BbvCI    BglII     BmtI
     BpmI   Bpu10I    BsaBI    BseRI     BsgI     BsmI    BspHI    BsrDI
    BstYI    CspCI     DraI     EarI  Eco57MI   HincII  HindIII     MmeI
   MspA1I     NheI     NspI     PciI     PpiI     PstI    PvuII     SapI
     ScaI     SfcI     SphI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI