Restriction map for
pF1KB5883
(Linear) (Six-base) MAPSORT of: KIBB5883.seq Check: 2861 from: 1 to: 1401
KIBB5883.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1401
Size: 82 1319
AhdI GACnn_n'nnGTC
Cuts at: 0 337 1401
Size: 337 1064
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1378 1401
Size: 1378 23
AlwNI CAG_nnn'CTG
Cuts at: 0 828 1074 1401
Size: 828 246 327
Fragments arranged by size:
828 327 246
ApaI G_GGCC'C
Cuts at: 0 20 303 720 1063 1401
Size: 20 283 417 343 338
Fragments arranged by size:
417 343 338 283 20
ApoI r'AATT_y
Cuts at: 0 352 1392 1401
Size: 352 1040 9
Fragments arranged by size:
1040 352 9
AvaI C'yCGr_G
Cuts at: 0 319 1401
Size: 319 1082
AvrII C'CTAG_G
Cuts at: 0 618 1401
Size: 618 783
BanII G_rGCy'C
Cuts at: 0 20 303 720 932 1063 1401
Size: 20 283 417 212 131 338
Fragments arranged by size:
417 338 283 212 131 20
BciVI GTATCCnnnnn_n'
Cuts at: 0 442 1401
Size: 442 959
BglI GCCn_nnn'nGGC
Cuts at: 0 225 848 1401
Size: 225 623 553
Fragments arranged by size:
623 553 225
Bme1580I G_kGCm'C
Cuts at: 0 20 152 303 617 720 796 1063 1401
Size: 20 132 151 314 103 76 267 338
Fragments arranged by size:
338 314 267 151 132 103 76 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 758 1401
Size: 723 35 643
Fragments arranged by size:
723 643 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1311 1401
Size: 1311 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 346 1072 1401
Size: 260 86 726 329
Fragments arranged by size:
726 329 260 86
Bpu10I CC'TnA_GC
Cuts at: 0 1181 1401
Size: 1181 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1401
Size: 1135 266
BsaI GGTCTCn'nnnn_
Cuts at: 0 453 1299 1401
Size: 453 846 102
Fragments arranged by size:
846 453 102
BsaWI w'CCGG_w
Cuts at: 0 774 1401
Size: 774 627
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 353 982 999 1008 1401
Size: 353 629 17 9 393
Fragments arranged by size:
629 393 353 17 9
BseYI C'CCAG_C
Cuts at: 0 74 204 228 392 1401
Size: 74 130 24 164 1009
Fragments arranged by size:
1009 164 130 74 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1109 1401
Size: 658 451 292
Fragments arranged by size:
658 451 292
BsiHKAI G_wGCw'C
Cuts at: 0 698 1260 1401
Size: 698 562 141
Fragments arranged by size:
698 562 141
Bsp1286I G_dGCh'C
Cuts at: 0 20 152 303 617 698 720 796 932
Size: 20 132 151 314 81 22 76 136
Cuts at: 932 1063 1260 1401
Size: 131 197 141
Fragments arranged by size:
314 197 151 141 136 132 131 81
76 22 20
BspEI T'CCGG_A
Cuts at: 0 774 1401
Size: 774 627
BsrDI GCAATG_nn'
Cuts at: 0 986 1401
Size: 986 415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 169 1401
Size: 169 1232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1290 1401
Size: 1290 111
BstYI r'GATC_y
Cuts at: 0 546 1101 1401
Size: 546 555 300
Fragments arranged by size:
555 546 300
BtgI C'CryG_G
Cuts at: 0 11 1401
Size: 11 1390
EaeI y'GGCC_r
Cuts at: 0 50 905 1401
Size: 50 855 496
Fragments arranged by size:
855 496 50
EarI CTCTTCn'nnn_
Cuts at: 0 180 228 410 1162 1401
Size: 180 48 182 752 239
Fragments arranged by size:
752 239 182 180 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 260 346 1072 1401
Size: 82 178 86 726 329
Fragments arranged by size:
726 329 178 86 82
EcoO109I rG'GnC_Cy
Cuts at: 0 16 66 99 217 261 339 704 717
Size: 16 50 33 118 44 78 365 13
Cuts at: 717 890 958 1060 1401
Size: 173 68 102 341
Fragments arranged by size:
365 341 173 118 102 78 68 50
44 33 16 13
EcoRI G'AATT_C
Cuts at: 0 352 1392 1401
Size: 352 1040 9
Fragments arranged by size:
1040 352 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 323 355 531 563 1047 1079 1401
Size: 323 32 176 32 484 32 322
Fragments arranged by size:
484 323 322 176 32 32 32
HincII GTy'rAC
Cuts at: 0 1095 1401
Size: 1095 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1241 1401
Size: 905 336 160
Fragments arranged by size:
905 336 160
MscI TGG'CCA
Cuts at: 0 52 907 1401
Size: 52 855 494
Fragments arranged by size:
855 494 52
MslI CAynn'nnrTG
Cuts at: 0 789 809 1401
Size: 789 20 592
Fragments arranged by size:
789 592 20
NspI r_CATG'y
Cuts at: 0 172 1401
Size: 172 1229
PasI CC'CwG_GG
Cuts at: 0 1192 1401
Size: 1192 209
PflMI CCAn_nnn'nTGG
Cuts at: 0 257 748 1192 1401
Size: 257 491 444 209
Fragments arranged by size:
491 444 257 209
PpuMI rG'GwC_Cy
Cuts at: 0 99 261 339 890 958 1401
Size: 99 162 78 551 68 443
Fragments arranged by size:
551 443 162 99 78 68
PspOMI G'GGCC_C
Cuts at: 0 16 299 716 1059 1401
Size: 16 283 417 343 342
Fragments arranged by size:
417 343 342 283 16
PstI C_TGCA'G
Cuts at: 0 46 1401
Size: 46 1355
SapI GCTCTTCn'nnn_
Cuts at: 0 228 1401
Size: 228 1173
SfcI C'TryA_G
Cuts at: 0 42 443 1265 1401
Size: 42 401 822 136
Fragments arranged by size:
822 401 136 42
SmlI C'TyrA_G
Cuts at: 0 1114 1401
Size: 1114 287
SphI G_CATG'C
Cuts at: 0 172 1401
Size: 172 1229
StyI C'CwwG_G
Cuts at: 0 94 618 1283 1401
Size: 94 524 665 118
Fragments arranged by size:
665 524 118 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 505 1401
Size: 321 184 896
Fragments arranged by size:
896 321 184
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApaI ApoI AvaI AvrII
BanII BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrDI BstAPI BstXI BstYI BtgI EaeI EarI
Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MscI MslI
NspI PasI PflMI PpuMI PspOMI PstI SapI SfcI
SmlI SphI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaLI AscI AseI AsiSI BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI