Restriction map for
pF1KB5887
(Linear) (Six-base) MAPSORT of: KIBB5887.seq Check: 1865 from: 1 to: 1491
KIBB5887.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 313 1491
Size: 313 1178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1445 1491
Size: 1331 114 46
Fragments arranged by size:
1331 114 46
AhdI GACnn_n'nnGTC
Cuts at: 0 1402 1491
Size: 1402 89
ApoI r'AATT_y
Cuts at: 0 64 318 744 753 1187 1478 1491
Size: 64 254 426 9 434 291 13
Fragments arranged by size:
434 426 291 254 64 13 9
AvaI C'yCGr_G
Cuts at: 0 462 1491
Size: 462 1029
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 20 53 1491
Size: 20 33 1438
Fragments arranged by size:
1438 33 20
BanI G'GyrC_C
Cuts at: 0 1325 1491
Size: 1325 166
BbeI G_GCGC'C
Cuts at: 0 1329 1491
Size: 1329 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 249 947 1491
Size: 249 698 544
Fragments arranged by size:
698 544 249
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 337 980 1491
Size: 267 70 643 511
Fragments arranged by size:
643 511 267 70
BglII A'GATC_T
Cuts at: 0 1236 1491
Size: 1236 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 929 1491
Size: 437 492 562
Fragments arranged by size:
562 492 437
Bpu10I CC'TnA_GC
Cuts at: 0 1321 1491
Size: 1321 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1240 1491
Size: 1063 177 251
Fragments arranged by size:
1063 251 177
BsaHI Gr'CG_yC
Cuts at: 0 1326 1491
Size: 1326 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 1491
Size: 164 30 1297
Fragments arranged by size:
1297 164 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 926 1060 1351 1491
Size: 926 134 291 140
Fragments arranged by size:
926 291 140 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1491
Size: 613 878
BsmI GAATG_Cn'
Cuts at: 0 1251 1491
Size: 1251 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 423 1491
Size: 48 375 1068
Fragments arranged by size:
1068 375 48
BssSI C'ACGA_G
Cuts at: 0 143 1491
Size: 143 1348
BstBI TT'CG_AA
Cuts at: 0 711 1491
Size: 711 780
BstEII G'GTnAC_C
Cuts at: 0 583 1491
Size: 583 908
BstYI r'GATC_y
Cuts at: 0 22 803 1236 1491
Size: 22 781 433 255
Fragments arranged by size:
781 433 255 22
BtgI C'CryG_G
Cuts at: 0 782 1491
Size: 782 709
BtsI GCAGTG_nn'
Cuts at: 0 601 1491
Size: 601 890
EaeI y'GGCC_r
Cuts at: 0 517 1491
Size: 517 974
EarI CTCTTCn'nnn_
Cuts at: 0 68 1307 1491
Size: 68 1239 184
Fragments arranged by size:
1239 184 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1445 1491
Size: 1331 114 46
Fragments arranged by size:
1331 114 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 387 1333 1491
Size: 387 946 158
Fragments arranged by size:
946 387 158
EcoRV GAT'ATC
Cuts at: 0 1196 1491
Size: 1196 295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1301 1491
Size: 1301 190
HaeII r_GCGC'y
Cuts at: 0 1329 1491
Size: 1329 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1388 1420 1491
Size: 379 32 977 32 71
Fragments arranged by size:
977 379 71 32 32
HincII GTy'rAC
Cuts at: 0 837 1434 1491
Size: 837 597 57
Fragments arranged by size:
837 597 57
HindIII A'AGCT_T
Cuts at: 0 1342 1491
Size: 1342 149
HpaI GTT'AAC
Cuts at: 0 837 1491
Size: 837 654
KasI G'GCGC_C
Cuts at: 0 1325 1491
Size: 1325 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1491
Size: 219 1272
MscI TGG'CCA
Cuts at: 0 519 1491
Size: 519 972
MspA1I CmG'CkG
Cuts at: 0 205 1491
Size: 205 1286
NarI GG'CG_CC
Cuts at: 0 1326 1491
Size: 1326 165
NcoI C'CATG_G
Cuts at: 0 782 1491
Size: 782 709
NspI r_CATG'y
Cuts at: 0 409 961 1491
Size: 409 552 530
Fragments arranged by size:
552 530 409
PshAI GACnn'nnGTC
Cuts at: 0 1439 1491
Size: 1439 52
PstI C_TGCA'G
Cuts at: 0 45 1491
Size: 45 1446
PvuII CAG'CTG
Cuts at: 0 205 1491
Size: 205 1286
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 1491
Size: 1307 184
ScaI AGT'ACT
Cuts at: 0 1308 1491
Size: 1308 183
SfcI C'TryA_G
Cuts at: 0 41 206 1491
Size: 41 165 1285
Fragments arranged by size:
1285 165 41
SfoI GGC'GCC
Cuts at: 0 1327 1491
Size: 1327 164
SmlI C'TyrA_G
Cuts at: 0 1042 1219 1491
Size: 1042 177 272
Fragments arranged by size:
1042 272 177
SspI AAT'ATT
Cuts at: 0 708 1023 1371 1491
Size: 708 315 348 120
Fragments arranged by size:
708 348 315 120
StyI C'CwwG_G
Cuts at: 0 782 946 1491
Size: 782 164 545
Fragments arranged by size:
782 545 164
TatI w'GTAC_w
Cuts at: 0 341 1306 1491
Size: 341 965 185
Fragments arranged by size:
965 341 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 164 196 921 953 1491
Size: 164 32 725 32 538
Fragments arranged by size:
725 538 164 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1187 1491
Size: 1187 304
Enzymes that do cut:
AccI AcuI AhdI ApoI AvaI BaeI BanI BbeI
BbsI BciVI BglII BmrI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsmI BspMI BssSI BstBI BstEII BstYI
BtgI BtsI EaeI EarI Eco57MI EcoNI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI
MspA1I NarI NcoI NspI PshAI PstI PvuII SapI
ScaI SfcI SfoI SmlI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BamHI BanII BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI FseI
FspI FspAI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI