(Linear) (Six-base) MAPSORT of: KIBB5888.seq  Check: 3876  from: 1  to: 1563



KIBB5888.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:      0     779    1563 
   Size:        779     784
 
Acc65I G'GTAC_C

Cuts at:      0    1510    1563 
   Size:       1510      53
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1514    1563 
   Size:       1514      49
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     485    1563 
   Size:        485    1078
 
ApoI r'AATT_y

Cuts at:      0    1552    1563 
   Size:       1552      11
 
AseI AT'TA_AT

Cuts at:      0    1105    1563 
   Size:       1105     458
 
BamHI G'GATC_C

Cuts at:      0     693    1563 
   Size:        693     870
 
BanI G'GyrC_C

Cuts at:      0     940    1510    1563 
   Size:        940     570      53

  Fragments arranged by size: 

                 940     570      53
 
BanII G_rGCy'C

Cuts at:      0     795    1563 
   Size:        795     768
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      84    1418    1563 
   Size:         84    1334     145

  Fragments arranged by size: 

                1334     145      84
 
Bme1580I G_kGCm'C

Cuts at:      0     860     872    1563 
   Size:        860      12     691

  Fragments arranged by size: 

                 860     691      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     247     601    1563 
   Size:        247     354     962

  Fragments arranged by size: 

                 962     354     247
 
Bpu10I CC'TnA_GC

Cuts at:      0    1133    1563 
   Size:       1133     430
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    1259    1563 
   Size:        434     825     304

  Fragments arranged by size: 

                 825     434     304
 
BsaBI GATnn'nnATC

Cuts at:      0     380    1475    1563 
   Size:        380    1095      88

  Fragments arranged by size: 

                1095     380      88
 
BseYI C'CCAG_C

Cuts at:      0      13    1563 
   Size:         13    1550
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     481    1563 
   Size:        425      56    1082

  Fragments arranged by size: 

                1082     425      56
 
BsmI GAATG_Cn'

Cuts at:      0     240    1563 
   Size:        240    1323
 
Bsp1286I G_dGCh'C

Cuts at:      0     795     860     872    1563 
   Size:        795      65      12     691

  Fragments arranged by size: 

                 795     691      65      12
 
BsrFI r'CCGG_y

Cuts at:      0    1324    1563 
   Size:       1324     239
 
BsrGI T'GTAC_A

Cuts at:      0    1221    1563 
   Size:       1221     342
 
BstYI r'GATC_y

Cuts at:      0     693    1476    1563 
   Size:        693     783      87

  Fragments arranged by size: 

                 783     693      87
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      74    1563 
   Size:         74    1489
 
BtsI GCAGTG_nn'

Cuts at:      0    1043    1563 
   Size:       1043     520
 
DraI TTT'AAA

Cuts at:      0     198    1563 
   Size:        198    1365
 
EaeI y'GGCC_r

Cuts at:      0       2    1322    1563 
   Size:          2    1320     241

  Fragments arranged by size: 

                1320     241       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     614    1563 
   Size:        614     949
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1514    1563 
   Size:       1514      49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1158    1563 
   Size:       1158     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     566     598     967     999    1107    1139    1563 
   Size:        566      32     369      32     108      32     424

  Fragments arranged by size: 

                 566     424     369     108      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1514    1563 
   Size:       1514      49
 
MfeI C'AATT_G

Cuts at:      0     262     969    1563 
   Size:        262     707     594

  Fragments arranged by size: 

                 707     594     262
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1076    1552    1563 
   Size:       1076     476      11

  Fragments arranged by size: 

                1076     476      11
 
MslI CAynn'nnrTG

Cuts at:      0      86     512    1406    1563 
   Size:         86     426     894     157

  Fragments arranged by size: 

                 894     426     157      86
 
PstI C_TGCA'G

Cuts at:      0     685    1563 
   Size:        685     878
 
SfcI C'TryA_G

Cuts at:      0     681    1563 
   Size:        681     882
 
SmlI C'TyrA_G

Cuts at:      0     449    1274    1563 
   Size:        449     825     289

  Fragments arranged by size: 

                 825     449     289
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1025    1563 
   Size:       1025     538
 
TatI w'GTAC_w

Cuts at:      0    1221    1563 
   Size:       1221     342
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1024    1056    1563 
   Size:       1024      32     507

  Fragments arranged by size: 

                1024     507      32
 
XbaI T'CTAG_A

Cuts at:      0     168     366    1293    1563 
   Size:        168     198     927     270

  Fragments arranged by size: 

                 927     270     198     168
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI     ApoI     AseI    BamHI     BanI
    BanII     BbsI Bme1580I     BmrI   Bpu10I    BpuEI    BsaBI    BseYI
     BsgI     BsmI Bsp1286I    BsrFI    BsrGI    BstYI    BtgZI     BtsI
     DraI     EaeI     EarI  Eco57MI     FalI    Hin4I     KpnI     MfeI
     MmeI     MslI     PstI     SfcI     SmlI    TaqII     TatI     TstI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI