Restriction map for
pF1KB5891
(Linear) (Six-base) MAPSORT of: KIBB5891.seq Check: 8141 from: 1 to: 1494
KIBB5891.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 966 1494
Size: 966 528
AccI GT'mk_AC
Cuts at: 0 215 1494
Size: 215 1279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1494
Size: 282 1212
AflIII A'CryG_T
Cuts at: 0 592 1494
Size: 592 902
AhdI GACnn_n'nnGTC
Cuts at: 0 1485 1494
Size: 1485 9
AleI CACnn'nnGTG
Cuts at: 0 682 1494
Size: 682 812
AlwNI CAG_nnn'CTG
Cuts at: 0 355 760 1117 1494
Size: 355 405 357 377
Fragments arranged by size:
405 377 357 355
ApaI G_GGCC'C
Cuts at: 0 1205 1494
Size: 1205 289
ApoI r'AATT_y
Cuts at: 0 442 631 817 1468 1494
Size: 442 189 186 651 26
Fragments arranged by size:
651 442 189 186 26
AvaI C'yCGr_G
Cuts at: 0 188 940 1494
Size: 188 752 554
Fragments arranged by size:
752 554 188
BamHI G'GATC_C
Cuts at: 0 246 1494
Size: 246 1248
BanI G'GyrC_C
Cuts at: 0 32 1494
Size: 32 1462
BanII G_rGCy'C
Cuts at: 0 161 1205 1494
Size: 161 1044 289
Fragments arranged by size:
1044 289 161
BciVI GTATCCnnnnn_n'
Cuts at: 0 680 1494
Size: 680 814
Bme1580I G_kGCm'C
Cuts at: 0 1205 1494
Size: 1205 289
BmgBI CAC'GTC
Cuts at: 0 1404 1494
Size: 1404 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 343 823 1494
Size: 343 480 671
Fragments arranged by size:
671 480 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1494
Size: 1013 481
BsaI GGTCTCn'nnnn_
Cuts at: 0 1031 1494
Size: 1031 463
BsaAI yAC'GTr
Cuts at: 0 580 595 1494
Size: 580 15 899
Fragments arranged by size:
899 580 15
BsaBI GATnn'nnATC
Cuts at: 0 991 1494
Size: 991 503
BsaHI Gr'CG_yC
Cuts at: 0 1036 1494
Size: 1036 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 377 794 899 902 1088 1091 1163 1166
Size: 377 417 105 3 186 3 72 3
Cuts at: 1166 1175 1178 1181 1184 1199 1375 1452 1494
Size: 9 3 3 3 15 176 77 42
Fragments arranged by size:
417 377 186 176 105 77 72 42
15 9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 117 654 1151 1494
Size: 117 537 497 343
Fragments arranged by size:
537 497 343 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1494
Size: 1247 247
BsiEI CG_ry'CG
Cuts at: 0 219 1494
Size: 219 1275
BsiHKAI G_wGCw'C
Cuts at: 0 161 1170 1494
Size: 161 1009 324
Fragments arranged by size:
1009 324 161
BsmI GAATG_Cn'
Cuts at: 0 82 1494
Size: 82 1412
Bsp1286I G_dGCh'C
Cuts at: 0 161 1170 1205 1494
Size: 161 1009 35 289
Fragments arranged by size:
1009 289 161 35
BspHI T'CATG_A
Cuts at: 0 512 1494
Size: 512 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 966 1325 1494
Size: 966 359 169
Fragments arranged by size:
966 359 169
BsrDI GCAATG_nn'
Cuts at: 0 1404 1427 1494
Size: 1404 23 67
Fragments arranged by size:
1404 67 23
BstYI r'GATC_y
Cuts at: 0 246 847 871 1494
Size: 246 601 24 623
Fragments arranged by size:
623 601 246 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1316 1494
Size: 1316 178
BtsI GCAGTG_nn'
Cuts at: 0 268 1494
Size: 268 1226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 662 697 1127 1162 1494
Size: 662 35 430 35 332
Fragments arranged by size:
662 430 332 35 35
EaeI y'GGCC_r
Cuts at: 0 1233 1406 1494
Size: 1233 173 88
Fragments arranged by size:
1233 173 88
EarI CTCTTCn'nnn_
Cuts at: 0 517 596 1494
Size: 517 79 898
Fragments arranged by size:
898 517 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 728 956 965 1494
Size: 728 228 9 529
Fragments arranged by size:
728 529 228 9
EcoICRI GAG'CTC
Cuts at: 0 159 1494
Size: 159 1335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1013 1494
Size: 282 731 481
Fragments arranged by size:
731 481 282
EcoNI CCTnn'n_nnAGG
Cuts at: 0 914 1494
Size: 914 580
EcoO109I rG'GnC_Cy
Cuts at: 0 931 1201 1202 1494
Size: 931 270 1 292
Fragments arranged by size:
931 292 270 1
EcoRI G'AATT_C
Cuts at: 0 817 1494
Size: 817 677
EcoRV GAT'ATC
Cuts at: 0 510 1494
Size: 510 984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1302 1494
Size: 1302 192
FspI TGC'GCA
Cuts at: 0 1351 1494
Size: 1351 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 1447 1471 1479 1494
Size: 325 32 1090 24 8 15
Fragments arranged by size:
1090 325 32 24 15 8
HincII GTy'rAC
Cuts at: 0 216 1494
Size: 216 1278
HindIII A'AGCT_T
Cuts at: 0 325 1494
Size: 325 1169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 1494
Size: 135 1359
MscI TGG'CCA
Cuts at: 0 1235 1408 1494
Size: 1235 173 86
Fragments arranged by size:
1235 173 86
MslI CAynn'nnrTG
Cuts at: 0 682 1494
Size: 682 812
MspA1I CmG'CkG
Cuts at: 0 117 243 1117 1347 1368 1494
Size: 117 126 874 230 21 126
Fragments arranged by size:
874 230 126 126 117 21
PspOMI G'GGCC_C
Cuts at: 0 1201 1494
Size: 1201 293
PstI C_TGCA'G
Cuts at: 0 497 1494
Size: 497 997
PvuII CAG'CTG
Cuts at: 0 117 243 1117 1347 1494
Size: 117 126 874 230 147
Fragments arranged by size:
874 230 147 126 117
SacI G_AGCT'C
Cuts at: 0 161 1494
Size: 161 1333
SalI G'TCGA_C
Cuts at: 0 214 1494
Size: 214 1280
SfcI C'TryA_G
Cuts at: 0 223 493 1013 1494
Size: 223 270 520 481
Fragments arranged by size:
520 481 270 223
SmaI CCC'GGG
Cuts at: 0 942 1494
Size: 942 552
StyI C'CwwG_G
Cuts at: 0 28 251 503 1494
Size: 28 223 252 991
Fragments arranged by size:
991 252 223 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 690 1494
Size: 233 457 804
Fragments arranged by size:
804 457 233
TatI w'GTAC_w
Cuts at: 0 1439 1494
Size: 1439 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 651 847 1494
Size: 93 558 196 647
Fragments arranged by size:
647 558 196 93
XmaI C'CCGG_G
Cuts at: 0 940 1494
Size: 940 554
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BciVI Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstYI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I
HincII HindIII MmeI MscI MslI MspA1I PspOMI PstI
PvuII SacI SalI SfcI SmaI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaWI BsaXI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraI DraIII DrdI
EagI FseI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI