Restriction map for
pF1KB5893
(Linear) (Six-base) MAPSORT of: KIBB5893.seq Check: 1041 from: 1 to: 1497
KIBB5893.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AatII G_ACGT'C
Cuts at: 0 1229 1497
Size: 1229 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1497
Size: 94 1403
AfeI AGC'GCT
Cuts at: 0 475 1497
Size: 475 1022
AleI CACnn'nnGTG
Cuts at: 0 676 1432 1497
Size: 676 756 65
Fragments arranged by size:
756 676 65
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1360 1392 1497
Size: 1360 32 105
Fragments arranged by size:
1360 105 32
AlwNI CAG_nnn'CTG
Cuts at: 0 636 1497
Size: 636 861
ApaI G_GGCC'C
Cuts at: 0 1139 1497
Size: 1139 358
AvaI C'yCGr_G
Cuts at: 0 44 1497
Size: 44 1453
BanII G_rGCy'C
Cuts at: 0 41 72 92 581 767 989 1139 1399
Size: 41 31 20 489 186 222 150 260
Cuts at: 1399 1497
Size: 98
Fragments arranged by size:
489 260 222 186 150 98 41 31
20
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 1079 1326 1497
Size: 551 528 247 171
Fragments arranged by size:
551 528 247 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 405 439 1497
Size: 405 34 1058
Fragments arranged by size:
1058 405 34
BclI T'GATC_A
Cuts at: 0 1046 1497
Size: 1046 451
BglI GCCn_nnn'nGGC
Cuts at: 0 259 1497
Size: 259 1238
BglII A'GATC_T
Cuts at: 0 932 1497
Size: 932 565
BlpI GC'TnA_GC
Cuts at: 0 35 667 1497
Size: 35 632 830
Fragments arranged by size:
830 632 35
Bme1580I G_kGCm'C
Cuts at: 0 1139 1497
Size: 1139 358
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 1017 1497
Size: 334 683 480
Fragments arranged by size:
683 480 334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 675 1497
Size: 675 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1497
Size: 1375 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1497
Size: 76 1421
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 1497
Size: 778 719
BsaHI Gr'CG_yC
Cuts at: 0 251 1226 1497
Size: 251 975 271
Fragments arranged by size:
975 271 251
BsaWI w'CCGG_w
Cuts at: 0 614 1097 1497
Size: 614 483 400
Fragments arranged by size:
614 483 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 811 841 1360 1390 1497
Size: 460 30 321 30 519 30 107
Fragments arranged by size:
519 460 321 107 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 739 1497
Size: 100 639 758
Fragments arranged by size:
758 639 100
BseYI C'CCAG_C
Cuts at: 0 175 243 1497
Size: 175 68 1254
Fragments arranged by size:
1254 175 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1497
Size: 595 902
BsiHKAI G_wGCw'C
Cuts at: 0 72 92 470 581 673 767 989 1497
Size: 72 20 378 111 92 94 222 508
Fragments arranged by size:
508 378 222 111 94 92 72 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 242 352 1497
Size: 242 110 1145
Fragments arranged by size:
1145 242 110
Bsp1286I G_dGCh'C
Cuts at: 0 41 72 92 470 581 673 767 989
Size: 41 31 20 378 111 92 94 222
Cuts at: 989 1139 1399 1497
Size: 150 260 98
Fragments arranged by size:
378 260 222 150 111 98 94 92
41 31 20
BspEI T'CCGG_A
Cuts at: 0 1097 1497
Size: 1097 400
BspHI T'CATG_A
Cuts at: 0 569 1497
Size: 569 928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1157 1497
Size: 224 933 340
Fragments arranged by size:
933 340 224
BsrBI CCG'CTC
Cuts at: 0 447 1110 1497
Size: 447 663 387
Fragments arranged by size:
663 447 387
BsrDI GCAATG_nn'
Cuts at: 0 186 1497
Size: 186 1311
BssSI C'ACGA_G
Cuts at: 0 676 910 1497
Size: 676 234 587
Fragments arranged by size:
676 587 234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 227 1497
Size: 227 1270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 318 1497
Size: 270 48 1179
Fragments arranged by size:
1179 270 48
BstYI r'GATC_y
Cuts at: 0 932 1161 1497
Size: 932 229 336
Fragments arranged by size:
932 336 229
BtgI C'CryG_G
Cuts at: 0 479 729 1457 1497
Size: 479 250 728 40
Fragments arranged by size:
728 479 250 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 438 1497
Size: 19 419 1059
Fragments arranged by size:
1059 419 19
BtsI GCAGTG_nn'
Cuts at: 0 592 1439 1497
Size: 592 847 58
Fragments arranged by size:
847 592 58
DraIII CAC_nnn'GTG
Cuts at: 0 227 1497
Size: 227 1270
EaeI y'GGCC_r
Cuts at: 0 732 1497
Size: 732 765
EarI CTCTTCn'nnn_
Cuts at: 0 1241 1497
Size: 1241 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1497
Size: 25 1472
EcoICRI GAG'CTC
Cuts at: 0 70 90 579 765 987 1497
Size: 70 20 489 186 222 510
Fragments arranged by size:
510 489 222 186 70 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1375 1497
Size: 94 1281 122
Fragments arranged by size:
1281 122 94
EcoO109I rG'GnC_Cy
Cuts at: 0 654 1135 1497
Size: 654 481 362
Fragments arranged by size:
654 481 362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1257 1497
Size: 1257 240
FspI TGC'GCA
Cuts at: 0 220 1497
Size: 220 1277
HaeII r_GCGC'y
Cuts at: 0 477 1185 1497
Size: 477 708 312
Fragments arranged by size:
708 477 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 811 843 1497
Size: 811 32 654
Fragments arranged by size:
811 654 32
HindIII A'AGCT_T
Cuts at: 0 538 1497
Size: 538 959
MscI TGG'CCA
Cuts at: 0 734 1497
Size: 734 763
MslI CAynn'nnrTG
Cuts at: 0 676 1432 1497
Size: 676 756 65
Fragments arranged by size:
756 676 65
MspA1I CmG'CkG
Cuts at: 0 530 1322 1497
Size: 530 792 175
Fragments arranged by size:
792 530 175
NcoI C'CATG_G
Cuts at: 0 729 1457 1497
Size: 729 728 40
Fragments arranged by size:
729 728 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 1497
Size: 636 861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1360 1392 1497
Size: 1168 32 160 32 105
Fragments arranged by size:
1168 160 105 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 654 1497
Size: 654 843
PshAI GACnn'nnGTC
Cuts at: 0 364 1497
Size: 364 1133
PspOMI G'GGCC_C
Cuts at: 0 1135 1497
Size: 1135 362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 919 951 1497
Size: 919 32 546
Fragments arranged by size:
919 546 32
PstI C_TGCA'G
Cuts at: 0 1484 1497
Size: 1484 13
SacI G_AGCT'C
Cuts at: 0 72 92 581 767 989 1497
Size: 72 20 489 186 222 508
Fragments arranged by size:
508 489 222 186 72 20
SfcI C'TryA_G
Cuts at: 0 150 836 1480 1497
Size: 150 686 644 17
Fragments arranged by size:
686 644 150 17
SmaI CCC'GGG
Cuts at: 0 46 1497
Size: 46 1451
SmlI C'TyrA_G
Cuts at: 0 91 1497
Size: 91 1406
StuI AGG'CCT
Cuts at: 0 439 1061 1237 1497
Size: 439 622 176 260
Fragments arranged by size:
622 439 260 176
StyI C'CwwG_G
Cuts at: 0 122 729 1232 1457 1497
Size: 122 607 503 225 40
Fragments arranged by size:
607 503 225 122 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 742 836 1497
Size: 742 94 661
Fragments arranged by size:
742 661 94
TatI w'GTAC_w
Cuts at: 0 281 524 965 1497
Size: 281 243 441 532
Fragments arranged by size:
532 441 281 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 460 492 1497
Size: 15 32 413 32 1005
Fragments arranged by size:
1005 413 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 0 1078 1497
Size: 1078 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1239 1497
Size: 1239 258
XmaI C'CCGG_G
Cuts at: 0 44 1497
Size: 44 1453
ZraI GAC'GTC
Cuts at: 0 1227 1497
Size: 1227 270
Enzymes that do cut:
AatII AcuI AfeI AleI AloI AlwNI ApaI AvaI
BanII BbsI BcgI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FspI HaeII Hin4I HindIII MscI MslI
MspA1I NcoI PflMI PpiI PpuMI PshAI PspOMI PsrI
PstI SacI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BfrBI BmgBI BmtI
Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoNI EcoRI EcoRV FseI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PsiI PspXI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI