Restriction map for
pF1KB5897
(Linear) (Six-base) MAPSORT of: KIBB5897.seq Check: 3244 from: 1 to: 2511
KIBB5897.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 825 2511
Size: 825 1686
AccI GT'mk_AC
Cuts at: 0 2066 2511
Size: 2066 445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 89 1166 1203 1813 2200 2344 2511
Size: 40 49 1077 37 610 387 144 167
Fragments arranged by size:
1077 610 387 167 144 49 40 37
AleI CACnn'nnGTG
Cuts at: 0 2243 2511
Size: 2243 268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 434 2511
Size: 421 13 2077
Fragments arranged by size:
2077 421 13
AlwNI CAG_nnn'CTG
Cuts at: 0 70 142 178 999 2249 2511
Size: 70 72 36 821 1250 262
Fragments arranged by size:
1250 821 262 72 70 36
ApaI G_GGCC'C
Cuts at: 0 188 2511
Size: 188 2323
ApoI r'AATT_y
Cuts at: 0 1072 2511
Size: 1072 1439
AvaI C'yCGr_G
Cuts at: 0 187 743 1036 1454 2070 2511
Size: 187 556 293 418 616 441
Fragments arranged by size:
616 556 441 418 293 187
BamHI G'GATC_C
Cuts at: 0 222 2511
Size: 222 2289
BanI G'GyrC_C
Cuts at: 0 132 1032 1896 2511
Size: 132 900 864 615
Fragments arranged by size:
900 864 615 132
BanII G_rGCy'C
Cuts at: 0 188 770 2078 2511
Size: 188 582 1308 433
Fragments arranged by size:
1308 582 433 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 315 1722 2332 2511
Size: 315 1407 610 179
Fragments arranged by size:
1407 610 315 179
BbvCI CC'TCA_GC
Cuts at: 0 845 2511
Size: 845 1666
BciVI GTATCCnnnnn_n'
Cuts at: 0 483 2511
Size: 483 2028
BclI T'GATC_A
Cuts at: 0 1658 2511
Size: 1658 853
BfrBI ATG'CAT
Cuts at: 0 623 2511
Size: 623 1888
BglI GCCn_nnn'nGGC
Cuts at: 0 1450 2511
Size: 1450 1061
BglII A'GATC_T
Cuts at: 0 1097 2511
Size: 1097 1414
BlpI GC'TnA_GC
Cuts at: 0 2077 2511
Size: 2077 434
Bme1580I G_kGCm'C
Cuts at: 0 188 905 1037 1901 2511
Size: 188 717 132 864 610
Fragments arranged by size:
864 717 610 188 132
BmgBI CAC'GTC
Cuts at: 0 852 2511
Size: 852 1659
BmrI ACTGGGnnnn_n'
Cuts at: 0 188 816 2237 2511
Size: 188 628 1421 274
Fragments arranged by size:
1421 628 274 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1345 1495 2482 2511
Size: 1108 237 150 987 29
Fragments arranged by size:
1108 987 237 150 29
Bpu10I CC'TnA_GC
Cuts at: 0 241 845 2511
Size: 241 604 1666
Fragments arranged by size:
1666 604 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 829 2470 2511
Size: 328 501 1641 41
Fragments arranged by size:
1641 501 328 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 842 2511
Size: 842 1669
BsaAI yAC'GTr
Cuts at: 0 471 900 2361 2511
Size: 471 429 1461 150
Fragments arranged by size:
1461 471 429 150
BsaHI Gr'CG_yC
Cuts at: 0 1525 2511
Size: 1525 986
BsaWI w'CCGG_w
Cuts at: 0 965 2511
Size: 965 1546
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 69 99 475 505 2511
Size: 69 30 376 30 2006
Fragments arranged by size:
2006 376 69 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1105 1108 1492 2511
Size: 1105 3 384 1019
Fragments arranged by size:
1105 1019 384 3
BseYI C'CCAG_C
Cuts at: 0 63 94 147 159 516 1204 1225 1416
Size: 63 31 53 12 357 688 21 191
Cuts at: 1416 2511
Size: 1095
Fragments arranged by size:
1095 688 357 191 63 53 31 21
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 374 736 1211 2511
Size: 74 300 362 475 1300
Fragments arranged by size:
1300 475 362 300 74
BsiHKAI G_wGCw'C
Cuts at: 0 455 770 2511
Size: 455 315 1741
Fragments arranged by size:
1741 455 315
BsmI GAATG_Cn'
Cuts at: 0 2129 2511
Size: 2129 382
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1420 2511
Size: 1420 1091
Bsp1286I G_dGCh'C
Cuts at: 0 188 455 770 905 1037 1901 2078 2511
Size: 188 267 315 135 132 864 177 433
Fragments arranged by size:
864 433 315 267 188 177 135 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 576 825 1746 2225 2396 2511
Size: 576 249 921 479 171 115
Fragments arranged by size:
921 576 479 249 171 115
BsrBI CCG'CTC
Cuts at: 0 215 750 2340 2511
Size: 215 535 1590 171
Fragments arranged by size:
1590 535 215 171
BsrFI r'CCGG_y
Cuts at: 0 163 989 1538 2511
Size: 163 826 549 973
Fragments arranged by size:
973 826 549 163
BssHII G'CGCG_C
Cuts at: 0 255 294 2511
Size: 255 39 2217
Fragments arranged by size:
2217 255 39
BssSI C'ACGA_G
Cuts at: 0 1558 2511
Size: 1558 953
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 2511
Size: 702 1809
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1371 2460 2511
Size: 1371 1089 51
Fragments arranged by size:
1371 1089 51
BstYI r'GATC_y
Cuts at: 0 222 402 1097 2054 2105 2511
Size: 222 180 695 957 51 406
Fragments arranged by size:
957 695 406 222 180 51
Bsu36I CC'TnA_GG
Cuts at: 0 1604 2511
Size: 1604 907
BtgI C'CryG_G
Cuts at: 0 1364 1485 2511
Size: 1364 121 1026
Fragments arranged by size:
1364 1026 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2385 2511
Size: 2385 126
BtsI GCAGTG_nn'
Cuts at: 0 386 2511
Size: 386 2125
EaeI y'GGCC_r
Cuts at: 0 1174 1442 2381 2511
Size: 1174 268 939 130
Fragments arranged by size:
1174 939 268 130
EarI CTCTTCn'nnn_
Cuts at: 0 550 560 607 1036 1291 2511
Size: 550 10 47 429 255 1220
Fragments arranged by size:
1220 550 429 255 47 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2511
Size: 19 2492
EcoICRI GAG'CTC
Cuts at: 0 768 2511
Size: 768 1743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 89 1108 1166 1203 1345 1495 1813
Size: 40 49 1019 58 37 142 150 318
Cuts at: 1813 2200 2344 2482 2511
Size: 387 144 138 29
Fragments arranged by size:
1019 387 318 150 144 142 138 58
49 40 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1286 2511
Size: 1286 1225
EcoO109I rG'GnC_Cy
Cuts at: 0 18 184 656 1046 1420 1772 2184 2283
Size: 18 166 472 390 374 352 412 99
Cuts at: 2283 2511
Size: 228
Fragments arranged by size:
472 412 390 374 352 228 166 99
18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 798 2511
Size: 798 1713
FspI TGC'GCA
Cuts at: 0 41 2511
Size: 41 2470
HaeII r_GCGC'y
Cuts at: 0 1485 2459 2511
Size: 1485 974 52
Fragments arranged by size:
1485 974 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 457 475 489 507 2511
Size: 105 32 320 18 14 18 2004
Fragments arranged by size:
2004 320 105 32 18 18 14
HindIII A'AGCT_T
Cuts at: 0 304 805 2511
Size: 304 501 1706
Fragments arranged by size:
1706 501 304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 142 2258 2511
Size: 106 36 2116 253
Fragments arranged by size:
2116 253 106 36
MslI CAynn'nnrTG
Cuts at: 0 1363 1894 2243 2511
Size: 1363 531 349 268
Fragments arranged by size:
1363 531 349 268
MspA1I CmG'CkG
Cuts at: 0 151 418 996 1164 1195 1487 1590 2081
Size: 151 267 578 168 31 292 103 491
Cuts at: 2081 2394 2447 2511
Size: 313 53 64
Fragments arranged by size:
578 491 313 292 267 168 151 103
64 53 31
NaeI GCC'GGC
Cuts at: 0 165 991 2511
Size: 165 826 1520
Fragments arranged by size:
1520 826 165
NcoI C'CATG_G
Cuts at: 0 1364 2511
Size: 1364 1147
NgoMIV G'CCGG_C
Cuts at: 0 163 989 2511
Size: 163 826 1522
Fragments arranged by size:
1522 826 163
NruI TCG'CGA
Cuts at: 0 2414 2511
Size: 2414 97
NsiI A_TGCA'T
Cuts at: 0 625 2511
Size: 625 1886
NspI r_CATG'y
Cuts at: 0 1602 1701 2511
Size: 1602 99 810
Fragments arranged by size:
1602 810 99
PasI CC'CwG_GG
Cuts at: 0 660 1710 2511
Size: 660 1050 801
Fragments arranged by size:
1050 801 660
PflMI CCAn_nnn'nTGG
Cuts at: 0 2249 2511
Size: 2249 262
PmlI CAC'GTG
Cuts at: 0 900 2511
Size: 900 1611
PpuMI rG'GwC_Cy
Cuts at: 0 656 1046 1772 2184 2511
Size: 656 390 726 412 327
Fragments arranged by size:
726 656 412 390 327
PspOMI G'GGCC_C
Cuts at: 0 184 2511
Size: 184 2327
PstI C_TGCA'G
Cuts at: 0 156 410 632 1272 1571 2393 2511
Size: 156 254 222 640 299 822 118
Fragments arranged by size:
822 640 299 254 222 156 118
PvuII CAG'CTG
Cuts at: 0 151 418 996 1164 1590 2081 2511
Size: 151 267 578 168 426 491 430
Fragments arranged by size:
578 491 430 426 267 168 151
SacI G_AGCT'C
Cuts at: 0 770 2511
Size: 770 1741
SacII CC_GC'GG
Cuts at: 0 1488 2511
Size: 1488 1023
SanDI GG'GwC_CC
Cuts at: 0 656 1772 2511
Size: 656 1116 739
Fragments arranged by size:
1116 739 656
ScaI AGT'ACT
Cuts at: 0 2440 2511
Size: 2440 71
SfcI C'TryA_G
Cuts at: 0 152 406 628 1268 1567 2389 2511
Size: 152 254 222 640 299 822 122
Fragments arranged by size:
822 640 299 254 222 152 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1450 2511
Size: 1450 1061
SmaI CCC'GGG
Cuts at: 0 189 745 1456 2072 2511
Size: 189 556 711 616 439
Fragments arranged by size:
711 616 556 439 189
SmlI C'TyrA_G
Cuts at: 0 307 808 2485 2511
Size: 307 501 1677 26
Fragments arranged by size:
1677 501 307 26
SphI G_CATG'C
Cuts at: 0 1602 2511
Size: 1602 909
SspI AAT'ATT
Cuts at: 0 690 2511
Size: 690 1821
StuI AGG'CCT
Cuts at: 0 2498 2511
Size: 2498 13
StyI C'CwwG_G
Cuts at: 0 458 1364 1529 1883 2511
Size: 458 906 165 354 628
Fragments arranged by size:
906 628 458 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 1222 1342 1723 2511
Size: 108 1114 120 381 788
Fragments arranged by size:
1114 788 381 120 108
TatI w'GTAC_w
Cuts at: 0 719 2417 2438 2511
Size: 719 1698 21 73
Fragments arranged by size:
1698 719 73 21
XmaI C'CCGG_G
Cuts at: 0 187 743 1454 2070 2511
Size: 187 556 711 616 441
Fragments arranged by size:
711 616 556 441 187
XmnI GAAnn'nnTTC
Cuts at: 0 544 2470 2511
Size: 544 1926 41
Fragments arranged by size:
1926 544 41
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HindIII MmeI MslI
MspA1I NaeI NcoI NgoMIV NruI NsiI NspI PasI
PflMI PmlI PpuMI PspOMI PstI PvuII SacI SacII
SanDI ScaI SfcI SfiI SmaI SmlI SphI SspI
StuI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BmtI BplI BsaBI BsiEI BsiWI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI NarI NdeI
NheI NotI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI
SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI
TstI Tth111I XbaI XcmI XhoI ZraI