Restriction map for
pF1KB5909
(Linear) (Six-base) MAPSORT of: KIBB5909.seq Check: 1504 from: 1 to: 1512
KIBB5909.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 1512
Size: 615 897
Acc65I G'GTAC_C
Cuts at: 0 112 1339 1512
Size: 112 1227 173
Fragments arranged by size:
1227 173 112
AflII C'TTAA_G
Cuts at: 0 1270 1512
Size: 1270 242
AleI CACnn'nnGTG
Cuts at: 0 44 805 1027 1438 1512
Size: 44 761 222 411 74
Fragments arranged by size:
761 411 222 74 44
AlwNI CAG_nnn'CTG
Cuts at: 0 285 831 1231 1512
Size: 285 546 400 281
Fragments arranged by size:
546 400 285 281
ApaI G_GGCC'C
Cuts at: 0 912 1180 1512
Size: 912 268 332
Fragments arranged by size:
912 332 268
ApaLI G'TGCA_C
Cuts at: 0 1064 1512
Size: 1064 448
AvaI C'yCGr_G
Cuts at: 0 380 1173 1512
Size: 380 793 339
Fragments arranged by size:
793 380 339
BamHI G'GATC_C
Cuts at: 0 668 1512
Size: 668 844
BanI G'GyrC_C
Cuts at: 0 112 1339 1512
Size: 112 1227 173
Fragments arranged by size:
1227 173 112
BanII G_rGCy'C
Cuts at: 0 611 912 970 1180 1512
Size: 611 301 58 210 332
Fragments arranged by size:
611 332 301 210 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 580 1512
Size: 580 932
BbvCI CC'TCA_GC
Cuts at: 0 198 934 1512
Size: 198 736 578
Fragments arranged by size:
736 578 198
BciVI GTATCCnnnnn_n'
Cuts at: 0 60 1512
Size: 60 1452
BclI T'GATC_A
Cuts at: 0 978 1512
Size: 978 534
BglI GCCn_nnn'nGGC
Cuts at: 0 1175 1512
Size: 1175 337
BglII A'GATC_T
Cuts at: 0 1124 1373 1512
Size: 1124 249 139
Fragments arranged by size:
1124 249 139
BlpI GC'TnA_GC
Cuts at: 0 610 1512
Size: 610 902
Bme1580I G_kGCm'C
Cuts at: 0 428 912 1068 1180 1363 1512
Size: 428 484 156 112 183 149
Fragments arranged by size:
484 428 183 156 149 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 996 1512
Size: 996 516
Bpu10I CC'TnA_GC
Cuts at: 0 198 934 964 1512
Size: 198 736 30 548
Fragments arranged by size:
736 548 198 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1480 1512
Size: 1480 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 1033 1512
Size: 1033 479
BsaAI yAC'GTr
Cuts at: 0 639 1512
Size: 639 873
BsaBI GATnn'nnATC
Cuts at: 0 1123 1378 1512
Size: 1123 255 134
Fragments arranged by size:
1123 255 134
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 601 960 1230 1512
Size: 160 441 359 270 282
Fragments arranged by size:
441 359 282 270 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1512
Size: 244 1268
BsiHKAI G_wGCw'C
Cuts at: 0 41 611 970 1022 1068 1512
Size: 41 570 359 52 46 444
Fragments arranged by size:
570 444 359 52 46 41
BsmI GAATG_Cn'
Cuts at: 0 659 1512
Size: 659 853
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 1512
Size: 361 1151
Bsp1286I G_dGCh'C
Cuts at: 0 41 428 611 912 970 1022 1068 1180
Size: 41 387 183 301 58 52 46 112
Cuts at: 1180 1363 1512
Size: 183 149
Fragments arranged by size:
387 301 183 183 149 112 58 52
46 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 1512
Size: 615 897
BsrBI CCG'CTC
Cuts at: 0 78 1512
Size: 78 1434
BsrDI GCAATG_nn'
Cuts at: 0 98 347 1512
Size: 98 249 1165
Fragments arranged by size:
1165 249 98
BsrFI r'CCGG_y
Cuts at: 0 620 1512
Size: 620 892
BsrGI T'GTAC_A
Cuts at: 0 894 1512
Size: 894 618
BstEII G'GTnAC_C
Cuts at: 0 798 1512
Size: 798 714
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1440 1512
Size: 1440 72
BstYI r'GATC_y
Cuts at: 0 668 707 1124 1257 1373 1512
Size: 668 39 417 133 116 139
Fragments arranged by size:
668 417 139 133 116 39
Bsu36I CC'TnA_GG
Cuts at: 0 584 1512
Size: 584 928
BtgI C'CryG_G
Cuts at: 0 551 1106 1512
Size: 551 555 406
Fragments arranged by size:
555 551 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1176 1452 1512
Size: 1176 276 60
Fragments arranged by size:
1176 276 60
BtsI GCAGTG_nn'
Cuts at: 0 371 1512
Size: 371 1141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 440 475 1512
Size: 440 35 1037
Fragments arranged by size:
1037 440 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1243 1512
Size: 1243 269
EaeI y'GGCC_r
Cuts at: 0 215 1154 1167 1512
Size: 215 939 13 345
Fragments arranged by size:
939 345 215 13
EarI CTCTTCn'nnn_
Cuts at: 0 559 599 1512
Size: 559 40 913
Fragments arranged by size:
913 559 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 739 1512
Size: 739 773
EcoICRI GAG'CTC
Cuts at: 0 609 968 1512
Size: 609 359 544
Fragments arranged by size:
609 544 359
EcoO109I rG'GnC_Cy
Cuts at: 0 828 1177 1512
Size: 828 349 335
Fragments arranged by size:
828 349 335
FspI TGC'GCA
Cuts at: 0 1264 1512
Size: 1264 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1225 1257 1300 1332 1512
Size: 1027 32 166 32 43 32 180
Fragments arranged by size:
1027 180 166 43 32 32 32
HindIII A'AGCT_T
Cuts at: 0 227 523 1267 1512
Size: 227 296 744 245
Fragments arranged by size:
744 296 245 227
KpnI G_GTAC'C
Cuts at: 0 116 1343 1512
Size: 116 1227 169
Fragments arranged by size:
1227 169 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1512
Size: 719 793
MscI TGG'CCA
Cuts at: 0 1156 1169 1512
Size: 1156 13 343
Fragments arranged by size:
1156 343 13
MslI CAynn'nnrTG
Cuts at: 0 44 71 805 917 1027 1438 1512
Size: 44 27 734 112 110 411 74
Fragments arranged by size:
734 411 112 110 74 44 27
MspA1I CmG'CkG
Cuts at: 0 285 1228 1293 1512
Size: 285 943 65 219
Fragments arranged by size:
943 285 219 65
NcoI C'CATG_G
Cuts at: 0 1106 1512
Size: 1106 406
PflMI CCAn_nnn'nTGG
Cuts at: 0 412 831 1512
Size: 412 419 681
Fragments arranged by size:
681 419 412
PmlI CAC'GTG
Cuts at: 0 639 1512
Size: 639 873
PpuMI rG'GwC_Cy
Cuts at: 0 828 1512
Size: 828 684
PspOMI G'GGCC_C
Cuts at: 0 908 1176 1512
Size: 908 268 336
Fragments arranged by size:
908 336 268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 887 919 1512
Size: 887 32 593
Fragments arranged by size:
887 593 32
PstI C_TGCA'G
Cuts at: 0 290 1512
Size: 290 1222
PvuII CAG'CTG
Cuts at: 0 285 1293 1512
Size: 285 1008 219
Fragments arranged by size:
1008 285 219
SacI G_AGCT'C
Cuts at: 0 611 970 1512
Size: 611 359 542
Fragments arranged by size:
611 542 359
SapI GCTCTTCn'nnn_
Cuts at: 0 599 1512
Size: 599 913
ScaI AGT'ACT
Cuts at: 0 985 1512
Size: 985 527
SexAI A'CCwGG_T
Cuts at: 0 830 1512
Size: 830 682
SfcI C'TryA_G
Cuts at: 0 286 1223 1503 1512
Size: 286 937 280 9
Fragments arranged by size:
937 286 280 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1175 1512
Size: 1175 337
SmlI C'TyrA_G
Cuts at: 0 1270 1495 1512
Size: 1270 225 17
Fragments arranged by size:
1270 225 17
StuI AGG'CCT
Cuts at: 0 932 1512
Size: 932 580
StyI C'CwwG_G
Cuts at: 0 362 1025 1106 1512
Size: 362 663 81 406
Fragments arranged by size:
663 406 362 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 191 451 475 864 1512
Size: 56 135 260 24 389 648
Fragments arranged by size:
648 389 260 135 56 24
TatI w'GTAC_w
Cuts at: 0 95 894 983 1512
Size: 95 799 89 529
Fragments arranged by size:
799 529 95 89
Enzymes that do cut:
AarI Acc65I AflII AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI
EcoICRI EcoO109I FspI Hin4I HindIII KpnI MmeI MscI
MslI MspA1I NcoI PflMI PmlI PpuMI PspOMI PsrI
PstI PvuII SacI SapI ScaI SexAI SfcI SfiI
SmlI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BfrBI BmgBI BmtI BplI BpmI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BspEI BspHI BssHII
BssSI BstAPI BstBI BstZ17I ClaI DraI DraIII EagI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII
HincII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PvuI
RsrII SacII SalI SanDI SbfI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI