Restriction map for
pF1KB5911
(Linear) (Six-base) MAPSORT of: KIBB5911.seq Check: 7186 from: 1 to: 1512
KIBB5911.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 905 1172 1176 1512
Size: 905 267 4 336
Fragments arranged by size:
905 336 267 4
AccI GT'mk_AC
Cuts at: 0 59 1512
Size: 59 1453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1512
Size: 316 1196
AfeI AGC'GCT
Cuts at: 0 1032 1512
Size: 1032 480
AflII C'TTAA_G
Cuts at: 0 532 1512
Size: 532 980
AleI CACnn'nnGTG
Cuts at: 0 1351 1512
Size: 1351 161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 1180 1512
Size: 307 873 332
Fragments arranged by size:
873 332 307
AlwNI CAG_nnn'CTG
Cuts at: 0 1118 1128 1212 1329 1512
Size: 1118 10 84 117 183
Fragments arranged by size:
1118 183 117 84 10
ApaI G_GGCC'C
Cuts at: 0 247 1503 1512
Size: 247 1256 9
Fragments arranged by size:
1256 247 9
AvaI C'yCGr_G
Cuts at: 0 561 1078 1477 1512
Size: 561 517 399 35
Fragments arranged by size:
561 517 399 35
BanI G'GyrC_C
Cuts at: 0 133 196 335 1005 1160 1512
Size: 133 63 139 670 155 352
Fragments arranged by size:
670 352 155 139 133 63
BanII G_rGCy'C
Cuts at: 0 247 413 461 692 944 1503 1512
Size: 247 166 48 231 252 559 9
Fragments arranged by size:
559 252 247 231 166 48 9
BbeI G_GCGC'C
Cuts at: 0 137 339 1512
Size: 137 202 1173
Fragments arranged by size:
1173 202 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 1512
Size: 22 1490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 692 726 1512
Size: 692 34 786
Fragments arranged by size:
786 692 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 715 1512
Size: 715 797
BglI GCCn_nnn'nGGC
Cuts at: 0 809 906 1173 1399 1512
Size: 809 97 267 226 113
Fragments arranged by size:
809 267 226 113 97
BlpI GC'TnA_GC
Cuts at: 0 1462 1512
Size: 1462 50
Bme1580I G_kGCm'C
Cuts at: 0 247 1010 1503 1512
Size: 247 763 493 9
Fragments arranged by size:
763 493 247 9
BmgBI CAC'GTC
Cuts at: 0 20 1512
Size: 20 1492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 876 1512
Size: 876 636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 910 946 1045 1078 1155 1512
Size: 460 450 36 99 33 77 357
Fragments arranged by size:
460 450 357 99 77 36 33
BsaAI yAC'GTr
Cuts at: 0 492 1512
Size: 492 1020
BsaHI Gr'CG_yC
Cuts at: 0 56 134 336 1512
Size: 56 78 202 1176
Fragments arranged by size:
1176 202 78 56
BsaWI w'CCGG_w
Cuts at: 0 1088 1512
Size: 1088 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 649 679 1153 1183 1458 1488
Size: 382 30 237 30 474 30 275 30
Cuts at: 1488 1512
Size: 24
Fragments arranged by size:
474 382 275 237 30 30 30 30
24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1324 1512
Size: 1324 188
BseYI C'CCAG_C
Cuts at: 0 1152 1284 1419 1512
Size: 1152 132 135 93
Fragments arranged by size:
1152 135 132 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1329 1512
Size: 235 1094 183
Fragments arranged by size:
1094 235 183
BsiEI CG_ry'CG
Cuts at: 0 143 1512
Size: 143 1369
BsiHKAI G_wGCw'C
Cuts at: 0 95 413 896 1468 1512
Size: 95 318 483 572 44
Fragments arranged by size:
572 483 318 95 44
Bsp1286I G_dGCh'C
Cuts at: 0 95 247 413 461 692 896 944 1010
Size: 95 152 166 48 231 204 48 66
Cuts at: 1010 1468 1503 1512
Size: 458 35 9
Fragments arranged by size:
458 231 204 166 152 95 66 48
48 35 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 653 905 959 1172 1176 1512
Size: 524 129 252 54 213 4 336
Fragments arranged by size:
524 336 252 213 129 54 4
BsrFI r'CCGG_y
Cuts at: 0 767 1512
Size: 767 745
BssHII G'CGCG_C
Cuts at: 0 1137 1383 1512
Size: 1137 246 129
Fragments arranged by size:
1137 246 129
BssSI C'ACGA_G
Cuts at: 0 607 1512
Size: 607 905
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1118 1512
Size: 1118 394
BstEII G'GTnAC_C
Cuts at: 0 168 618 1512
Size: 168 450 894
Fragments arranged by size:
894 450 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 1512
Size: 165 1347
BtgI C'CryG_G
Cuts at: 0 158 807 944 992 1412 1512
Size: 158 649 137 48 420 100
Fragments arranged by size:
649 420 158 137 100 48
BtsI GCAGTG_nn'
Cuts at: 0 839 1252 1512
Size: 839 413 260
Fragments arranged by size:
839 413 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 360 1512
Size: 360 1152
EaeI y'GGCC_r
Cuts at: 0 110 140 155 989 995 1298 1442 1512
Size: 110 30 15 834 6 303 144 70
Fragments arranged by size:
834 303 144 110 70 30 15 6
EagI C'GGCC_G
Cuts at: 0 140 1512
Size: 140 1372
EarI CTCTTCn'nnn_
Cuts at: 0 682 1345 1512
Size: 682 663 167
Fragments arranged by size:
682 663 167
EcoICRI GAG'CTC
Cuts at: 0 411 1512
Size: 411 1101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 460 910 946 1045 1078 1155 1512
Size: 316 144 450 36 99 33 77 357
Fragments arranged by size:
450 357 316 144 99 77 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 295 667 925 1512
Size: 187 108 372 258 587
Fragments arranged by size:
587 372 258 187 108
EcoO109I rG'GnC_Cy
Cuts at: 0 244 300 672 918 1499 1500 1512
Size: 244 56 372 246 581 1 12
Fragments arranged by size:
581 372 246 244 56 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1512
Size: 693 819
FspI TGC'GCA
Cuts at: 0 649 1270 1378 1512
Size: 649 621 108 134
Fragments arranged by size:
649 621 134 108
HaeII r_GCGC'y
Cuts at: 0 137 151 166 339 845 1034 1323 1512
Size: 137 14 15 173 506 189 289 189
Fragments arranged by size:
506 289 189 189 173 137 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 1512
Size: 1408 32 72
Fragments arranged by size:
1408 72 32
KasI G'GCGC_C
Cuts at: 0 133 335 1512
Size: 133 202 1177
Fragments arranged by size:
1177 202 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1512
Size: 992 520
MscI TGG'CCA
Cuts at: 0 112 991 1300 1512
Size: 112 879 309 212
Fragments arranged by size:
879 309 212 112
MslI CAynn'nnrTG
Cuts at: 0 323 329 445 547 997 1003 1351 1512
Size: 323 6 116 102 450 6 348 161
Fragments arranged by size:
450 348 323 161 116 102 6 6
MspA1I CmG'CkG
Cuts at: 0 434 750 867 1023 1227 1326 1414 1440
Size: 434 316 117 156 204 99 88 26
Cuts at: 1440 1512
Size: 72
Fragments arranged by size:
434 316 204 156 117 99 88 72
26
NarI GG'CG_CC
Cuts at: 0 134 336 1512
Size: 134 202 1176
Fragments arranged by size:
1176 202 134
NspI r_CATG'y
Cuts at: 0 328 975 1383 1512
Size: 328 647 408 129
Fragments arranged by size:
647 408 328 129
PasI CC'CwG_GG
Cuts at: 0 1503 1512
Size: 1503 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 1128 1512
Size: 834 294 384
Fragments arranged by size:
834 384 294
PfoI T'CCnGG_A
Cuts at: 0 887 1512
Size: 887 625
PpuMI rG'GwC_Cy
Cuts at: 0 300 1512
Size: 300 1212
PshAI GACnn'nnGTC
Cuts at: 0 578 1512
Size: 578 934
PspOMI G'GGCC_C
Cuts at: 0 243 1499 1512
Size: 243 1256 13
Fragments arranged by size:
1256 243 13
PstI C_TGCA'G
Cuts at: 0 433 749 1512
Size: 433 316 763
Fragments arranged by size:
763 433 316
PvuII CAG'CTG
Cuts at: 0 867 1326 1440 1512
Size: 867 459 114 72
Fragments arranged by size:
867 459 114 72
SacI G_AGCT'C
Cuts at: 0 413 1512
Size: 413 1099
SacII CC_GC'GG
Cuts at: 0 1415 1512
Size: 1415 97
SexAI A'CCwGG_T
Cuts at: 0 266 1127 1512
Size: 266 861 385
Fragments arranged by size:
861 385 266
SfcI C'TryA_G
Cuts at: 0 429 745 1242 1512
Size: 429 316 497 270
Fragments arranged by size:
497 429 316 270
SfoI GGC'GCC
Cuts at: 0 135 337 1512
Size: 135 202 1175
Fragments arranged by size:
1175 202 135
SmlI C'TyrA_G
Cuts at: 0 532 1512
Size: 532 980
SphI G_CATG'C
Cuts at: 0 328 1383 1512
Size: 328 1055 129
Fragments arranged by size:
1055 328 129
StuI AGG'CCT
Cuts at: 0 931 1512
Size: 931 581
StyI C'CwwG_G
Cuts at: 0 401 668 1512
Size: 401 267 844
Fragments arranged by size:
844 401 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 1512
Size: 175 1337
TatI w'GTAC_w
Cuts at: 0 449 1512
Size: 449 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 1075 1107 1153 1185 1458 1490
Size: 382 32 661 32 46 32 273 32
Cuts at: 1490 1512
Size: 22
Fragments arranged by size:
661 382 273 46 32 32 32 32
22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 780 999 1053 1149 1512
Size: 336 444 219 54 96 363
Fragments arranged by size:
444 363 336 219 96 54
XmnI GAAnn'nnTTC
Cuts at: 0 235 1512
Size: 235 1277
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AleI AlfI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BcgI BciVI
BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BtgI
BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SexAI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EciI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI