Restriction map for
pF1KB5912
(Linear) (Six-base) MAPSORT of: KIBB5912.seq Check: 3349 from: 1 to: 1587
KIBB5912.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 1587
Size: 559 1028
AclI AA'CG_TT
Cuts at: 0 1514 1587
Size: 1514 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1556 1587
Size: 163 1393 31
Fragments arranged by size:
1393 163 31
AleI CACnn'nnGTG
Cuts at: 0 533 1587
Size: 533 1054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 1587
Size: 828 759
AlwNI CAG_nnn'CTG
Cuts at: 0 141 646 1587
Size: 141 505 941
Fragments arranged by size:
941 505 141
ApoI r'AATT_y
Cuts at: 0 367 1587
Size: 367 1220
BanI G'GyrC_C
Cuts at: 0 484 1587
Size: 484 1103
BanII G_rGCy'C
Cuts at: 0 555 1569 1587
Size: 555 1014 18
Fragments arranged by size:
1014 555 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 1587
Size: 31 1556
BbvCI CC'TCA_GC
Cuts at: 0 112 1587
Size: 112 1475
Bme1580I G_kGCm'C
Cuts at: 0 217 429 887 1587
Size: 217 212 458 700
Fragments arranged by size:
700 458 217 212
Bpu10I CC'TnA_GC
Cuts at: 0 112 1587
Size: 112 1475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1587
Size: 275 1312
BsaAI yAC'GTr
Cuts at: 0 679 1067 1587
Size: 679 388 520
Fragments arranged by size:
679 520 388
BsaHI Gr'CG_yC
Cuts at: 0 84 1587
Size: 84 1503
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 1587
Size: 162 1425
BseYI C'CCAG_C
Cuts at: 0 492 815 1021 1587
Size: 492 323 206 566
Fragments arranged by size:
566 492 323 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 710 1587
Size: 632 78 877
Fragments arranged by size:
877 632 78
BsiEI CG_ry'CG
Cuts at: 0 1095 1587
Size: 1095 492
BsiHKAI G_wGCw'C
Cuts at: 0 1008 1587
Size: 1008 579
BsmI GAATG_Cn'
Cuts at: 0 21 496 1587
Size: 21 475 1091
Fragments arranged by size:
1091 475 21
Bsp1286I G_dGCh'C
Cuts at: 0 217 429 555 887 1008 1569 1587
Size: 217 212 126 332 121 561 18
Fragments arranged by size:
561 332 217 212 126 121 18
BspHI T'CATG_A
Cuts at: 0 783 1587
Size: 783 804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 820 1587
Size: 559 261 767
Fragments arranged by size:
767 559 261
BsrDI GCAATG_nn'
Cuts at: 0 270 437 563 1587
Size: 270 167 126 1024
Fragments arranged by size:
1024 270 167 126
BsrFI r'CCGG_y
Cuts at: 0 1090 1587
Size: 1090 497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1587
Size: 471 1116
EaeI y'GGCC_r
Cuts at: 0 1092 1587
Size: 1092 495
EagI C'GGCC_G
Cuts at: 0 1092 1587
Size: 1092 495
EarI CTCTTCn'nnn_
Cuts at: 0 139 1493 1587
Size: 139 1354 94
Fragments arranged by size:
1354 139 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1556 1587
Size: 163 1393 31
Fragments arranged by size:
1393 163 31
EcoO109I rG'GnC_Cy
Cuts at: 0 1574 1587
Size: 1574 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 473 505 640 672 1587
Size: 473 32 135 32 915
Fragments arranged by size:
915 473 135 32 32
HincII GTy'rAC
Cuts at: 0 618 1587
Size: 618 969
HindIII A'AGCT_T
Cuts at: 0 898 1467 1587
Size: 898 569 120
Fragments arranged by size:
898 569 120
MfeI C'AATT_G
Cuts at: 0 923 1587
Size: 923 664
MslI CAynn'nnrTG
Cuts at: 0 533 785 865 1439 1587
Size: 533 252 80 574 148
Fragments arranged by size:
574 533 252 148 80
MspA1I CmG'CkG
Cuts at: 0 31 189 1587
Size: 31 158 1398
Fragments arranged by size:
1398 158 31
NaeI GCC'GGC
Cuts at: 0 1092 1587
Size: 1092 495
NgoMIV G'CCGG_C
Cuts at: 0 1090 1587
Size: 1090 497
NspI r_CATG'y
Cuts at: 0 668 1587
Size: 668 919
PfoI T'CCnGG_A
Cuts at: 0 1319 1587
Size: 1319 268
PpuMI rG'GwC_Cy
Cuts at: 0 1574 1587
Size: 1574 13
PstI C_TGCA'G
Cuts at: 0 194 1587
Size: 194 1393
PvuII CAG'CTG
Cuts at: 0 31 1587
Size: 31 1556
SfcI C'TryA_G
Cuts at: 0 190 1490 1587
Size: 190 1300 97
Fragments arranged by size:
1300 190 97
SmlI C'TyrA_G
Cuts at: 0 290 1587
Size: 290 1297
SnaBI TAC'GTA
Cuts at: 0 679 1587
Size: 679 908
SspI AAT'ATT
Cuts at: 0 957 1587
Size: 957 630
StyI C'CwwG_G
Cuts at: 0 464 1587
Size: 464 1123
TatI w'GTAC_w
Cuts at: 0 70 1587
Size: 70 1517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1177 1209 1587
Size: 1051 32 94 32 378
Fragments arranged by size:
1051 378 94 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 241 1074 1587
Size: 241 833 513
Fragments arranged by size:
833 513 241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1308 1558 1587
Size: 1308 250 29
Fragments arranged by size:
1308 250 29
XmnI GAAnn'nnTTC
Cuts at: 0 1483 1587
Size: 1483 104
Enzymes that do cut:
AarI AclI AcuI AleI AlfI AlwNI ApoI BanI
BanII BbsI BbvCI Bme1580I Bpu10I BpuEI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BstXI EaeI EagI EarI Eco57MI
EcoO109I Hin4I HincII HindIII MfeI MslI MspA1I NaeI
NgoMIV NspI PfoI PpuMI PstI PvuII SfcI SmlI
SnaBI SspI StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaI
BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI StuI SwaI TaqII
XbaI XhoI XmaI ZraI