(Linear) (Six-base) MAPSORT of: KIBB5919.seq Check: 5391 from: 1 to: 1515 KIBB5919.orf With 182 enzymes: SgfI * April 24, 2009 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 184 1515 Size: 184 1331 AccI GT'mk_AC Cuts at: 0 995 1515 Size: 995 520 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 1515 Size: 730 785 AgeI A'CCGG_T Cuts at: 0 409 1515 Size: 409 1106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 29 61 1515 Size: 29 32 1454 Fragments arranged by size: 1454 32 29 AlwNI CAG_nnn'CTG Cuts at: 0 708 1515 Size: 708 807 ApaI G_GGCC'C Cuts at: 0 102 1515 Size: 102 1413 ApoI r'AATT_y Cuts at: 0 451 562 1225 1515 Size: 451 111 663 290 Fragments arranged by size: 663 451 290 111 BamHI G'GATC_C Cuts at: 0 807 1515 Size: 807 708 BanI G'GyrC_C Cuts at: 0 1315 1515 Size: 1315 200 BanII G_rGCy'C Cuts at: 0 102 1515 Size: 102 1413 BbsI GAAGACnn'nnnn_ Cuts at: 0 1157 1469 1515 Size: 1157 312 46 Fragments arranged by size: 1157 312 46 BbvCI CC'TCA_GC Cuts at: 0 173 317 1515 Size: 173 144 1198 Fragments arranged by size: 1198 173 144 BglII A'GATC_T Cuts at: 0 1059 1515 Size: 1059 456 Bme1580I G_kGCm'C Cuts at: 0 102 1320 1515 Size: 102 1218 195 Fragments arranged by size: 1218 195 102 BmrI ACTGGGnnnn_n' Cuts at: 0 108 1515 Size: 108 1407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 755 1054 1515 Size: 258 497 299 461 Fragments arranged by size: 497 461 299 258 Bpu10I CC'TnA_GC Cuts at: 0 173 317 1515 Size: 173 144 1198 Fragments arranged by size: 1198 173 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 1515 Size: 201 1314 BsaAI yAC'GTr Cuts at: 0 312 1515 Size: 312 1203 BsaBI GATnn'nnATC Cuts at: 0 720 1515 Size: 720 795 BsaWI w'CCGG_w Cuts at: 0 409 1515 Size: 409 1106 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 29 59 467 497 679 709 1515 Size: 29 30 408 30 182 30 806 Fragments arranged by size: 806 408 182 30 30 30 29 BseYI C'CCAG_C Cuts at: 0 609 639 1515 Size: 609 30 876 Fragments arranged by size: 876 609 30 BsmI GAATG_Cn' Cuts at: 0 1416 1515 Size: 1416 99 Bsp1286I G_dGCh'C Cuts at: 0 102 1320 1515 Size: 102 1218 195 Fragments arranged by size: 1218 195 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 184 1515 Size: 184 1331 BsrFI r'CCGG_y Cuts at: 0 409 1185 1515 Size: 409 776 330 Fragments arranged by size: 776 409 330 BstAPI GCAn_nnn'nTGC Cuts at: 0 1012 1515 Size: 1012 503 BstXI CCAn_nnnn'nTGG Cuts at: 0 252 1515 Size: 252 1263 BstYI r'GATC_y Cuts at: 0 629 721 807 897 1059 1515 Size: 629 92 86 90 162 456 Fragments arranged by size: 629 456 162 92 90 86 Bsu36I CC'TnA_GG Cuts at: 0 823 1421 1515 Size: 823 598 94 Fragments arranged by size: 823 598 94 BtgI C'CryG_G Cuts at: 0 59 343 668 1515 Size: 59 284 325 847 Fragments arranged by size: 847 325 284 59 BtsI GCAGTG_nn' Cuts at: 0 785 1444 1515 Size: 785 659 71 Fragments arranged by size: 785 659 71 DrdI GACnn_nn'nnGTC Cuts at: 0 935 1515 Size: 935 580 EaeI y'GGCC_r Cuts at: 0 129 1515 Size: 129 1386 EarI CTCTTCn'nnn_ Cuts at: 0 1488 1515 Size: 1488 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 730 755 1054 1515 Size: 258 472 25 299 461 Fragments arranged by size: 472 461 299 258 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 826 1515 Size: 826 689 EcoO109I rG'GnC_Cy Cuts at: 0 98 99 636 1515 Size: 98 1 537 879 Fragments arranged by size: 879 537 98 1 EcoRI G'AATT_C Cuts at: 0 451 1515 Size: 451 1064 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 75 107 512 544 679 711 952 959 Size: 75 32 405 32 135 32 241 7 Cuts at: 959 984 991 1234 1266 1323 1355 1515 Size: 25 7 243 32 57 32 160 Fragments arranged by size: 405 243 241 160 135 75 57 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 0 77 1515 Size: 77 1438 HindIII A'AGCT_T Cuts at: 0 862 1515 Size: 862 653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1379 1391 1432 1515 Size: 1379 12 41 83 Fragments arranged by size: 1379 83 41 12 MscI TGG'CCA Cuts at: 0 131 1515 Size: 131 1384 PflMI CCAn_nnn'nTGG Cuts at: 0 1335 1515 Size: 1335 180 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 29 61 331 363 1515 Size: 29 32 270 32 1152 Fragments arranged by size: 1152 270 32 32 29 PpuMI rG'GwC_Cy Cuts at: 0 636 1515 Size: 636 879 PspOMI G'GGCC_C Cuts at: 0 98 1515 Size: 98 1417 SexAI A'CCwGG_T Cuts at: 0 795 1515 Size: 795 720 SfcI C'TryA_G Cuts at: 0 331 492 1347 1515 Size: 331 161 855 168 Fragments arranged by size: 855 331 168 161 SmlI C'TyrA_G Cuts at: 0 180 1515 Size: 180 1335 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 739 771 1515 Size: 739 32 744 Fragments arranged by size: 744 739 32 XbaI T'CTAG_A Cuts at: 0 1165 1515 Size: 1165 350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 961 1515 Size: 961 554 XmnI GAAnn'nnTTC Cuts at: 0 546 1211 1515 Size: 546 665 304 Fragments arranged by size: 665 546 304 Enzymes that do cut: AarI AccI AcuI AgeI AloI AlwNI ApaI ApoI BamHI BanI BanII BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsmI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MmeI MscI PflMI PpiI PpuMI PspOMI SexAI SfcI SmlI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XhoI XmaI ZraI