Restriction map for
pF1KB5925
(Linear) (Six-base) MAPSORT of: KIBB5925.seq Check: 712 from: 1 to: 1512
KIBB5925.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 861 1512
Size: 861 651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 630 1512
Size: 70 560 882
Fragments arranged by size:
882 560 70
AfeI AGC'GCT
Cuts at: 0 949 1512
Size: 949 563
AlwNI CAG_nnn'CTG
Cuts at: 0 720 969 1512
Size: 720 249 543
Fragments arranged by size:
720 543 249
ApaI G_GGCC'C
Cuts at: 0 320 683 807 1484 1512
Size: 320 363 124 677 28
Fragments arranged by size:
677 363 320 124 28
AvaI C'yCGr_G
Cuts at: 0 320 800 1241 1512
Size: 320 480 441 271
Fragments arranged by size:
480 441 320 271
BanI G'GyrC_C
Cuts at: 0 1272 1512
Size: 1272 240
BanII G_rGCy'C
Cuts at: 0 320 683 807 992 1154 1400 1484 1512
Size: 320 363 124 185 162 246 84 28
Fragments arranged by size:
363 320 246 185 162 124 84 28
BbvCI CC'TCA_GC
Cuts at: 0 335 1071 1512
Size: 335 736 441
Fragments arranged by size:
736 441 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 948 982 1512
Size: 948 34 530
Fragments arranged by size:
948 530 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1348 1512
Size: 1348 164
BclI T'GATC_A
Cuts at: 0 734 1512
Size: 734 778
BglI GCCn_nnn'nGGC
Cuts at: 0 247 1512
Size: 247 1265
BglII A'GATC_T
Cuts at: 0 347 1199 1349 1512
Size: 347 852 150 163
Fragments arranged by size:
852 347 163 150
Bme1580I G_kGCm'C
Cuts at: 0 279 320 683 807 1484 1512
Size: 279 41 363 124 677 28
Fragments arranged by size:
677 363 279 124 41 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 10 929 1512
Size: 10 919 583
Fragments arranged by size:
919 583 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 423 675 1323 1512
Size: 378 45 252 648 189
Fragments arranged by size:
648 378 252 189 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 584 691 787 874 919 982 1074
Size: 159 425 107 96 87 45 63 92
Cuts at: 1074 1153 1318 1475 1512
Size: 79 165 157 37
Fragments arranged by size:
425 165 159 157 107 96 92 87
79 63 45 37
Bpu10I CC'TnA_GC
Cuts at: 0 335 469 1071 1512
Size: 335 134 602 441
Fragments arranged by size:
602 441 335 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1512
Size: 760 30 722
Fragments arranged by size:
760 722 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 442 611 1512
Size: 439 3 169 901
Fragments arranged by size:
901 439 169 3
BseYI C'CCAG_C
Cuts at: 0 163 389 585 607 916 1418 1497 1512
Size: 163 226 196 22 309 502 79 15
Fragments arranged by size:
502 309 226 196 163 79 22 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 508 1512
Size: 437 71 1004
Fragments arranged by size:
1004 437 71
BsiHKAI G_wGCw'C
Cuts at: 0 1154 1400 1512
Size: 1154 246 112
Fragments arranged by size:
1154 246 112
Bsp1286I G_dGCh'C
Cuts at: 0 279 320 683 807 992 1154 1400 1484
Size: 279 41 363 124 185 162 246 84
Cuts at: 1484 1512
Size: 28
Fragments arranged by size:
363 279 246 185 162 124 84 41
28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 737 861 1512
Size: 275 462 124 651
Fragments arranged by size:
651 462 275 124
BsrBI CCG'CTC
Cuts at: 0 1058 1512
Size: 1058 454
BsrFI r'CCGG_y
Cuts at: 0 833 1512
Size: 833 679
BsrGI T'GTAC_A
Cuts at: 0 1263 1512
Size: 1263 249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1428 1512
Size: 1428 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 615 897 1512
Size: 18 597 282 615
Fragments arranged by size:
615 597 282 18
BstYI r'GATC_y
Cuts at: 0 347 1199 1349 1512
Size: 347 852 150 163
Fragments arranged by size:
852 347 163 150
Bsu36I CC'TnA_GG
Cuts at: 0 398 923 1512
Size: 398 525 589
Fragments arranged by size:
589 525 398
BtgI C'CryG_G
Cuts at: 0 578 825 984 1512
Size: 578 247 159 528
Fragments arranged by size:
578 528 247 159
BtsI GCAGTG_nn'
Cuts at: 0 491 1512
Size: 491 1021
EaeI y'GGCC_r
Cuts at: 0 248 541 793 1473 1512
Size: 248 293 252 680 39
Fragments arranged by size:
680 293 252 248 39
EarI CTCTTCn'nnn_
Cuts at: 0 431 437 844 886 1512
Size: 431 6 407 42 626
Fragments arranged by size:
626 431 407 42 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 289 1018 1512
Size: 289 729 494
Fragments arranged by size:
729 494 289
EcoICRI GAG'CTC
Cuts at: 0 1152 1398 1512
Size: 1152 246 114
Fragments arranged by size:
1152 246 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 159 584 630 691 787 874 919
Size: 70 89 425 46 61 96 87 45
Cuts at: 919 982 1074 1153 1318 1475 1512
Size: 63 92 79 165 157 37
Fragments arranged by size:
425 165 157 96 92 89 87 79
70 63 61 46 45 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1457 1512
Size: 1457 55
EcoO109I rG'GnC_Cy
Cuts at: 0 317 589 666 680 804 1014 1446 1480
Size: 317 272 77 14 124 210 432 34
Cuts at: 1480 1481 1512
Size: 1 31
Fragments arranged by size:
432 317 272 210 124 77 34 31
14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 966 1512
Size: 966 546
HaeII r_GCGC'y
Cuts at: 0 951 1512
Size: 951 561
HincII GTy'rAC
Cuts at: 0 108 1512
Size: 108 1404
HindIII A'AGCT_T
Cuts at: 0 1120 1512
Size: 1120 392
MscI TGG'CCA
Cuts at: 0 1475 1512
Size: 1475 37
MslI CAynn'nnrTG
Cuts at: 0 16 1512
Size: 16 1496
MspA1I CmG'CkG
Cuts at: 0 80 852 966 986 1104 1330 1425 1501
Size: 80 772 114 20 118 226 95 76
Cuts at: 1501 1512
Size: 11
Fragments arranged by size:
772 226 118 114 95 80 76 20
11
NaeI GCC'GGC
Cuts at: 0 835 1512
Size: 835 677
NcoI C'CATG_G
Cuts at: 0 825 1512
Size: 825 687
NgoMIV G'CCGG_C
Cuts at: 0 833 1512
Size: 833 679
NspI r_CATG'y
Cuts at: 0 148 330 750 1509 1512
Size: 148 182 420 759 3
Fragments arranged by size:
759 420 182 148 3
PasI CC'CwG_GG
Cuts at: 0 91 1512
Size: 91 1421
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 1512
Size: 969 543
PfoI T'CCnGG_A
Cuts at: 0 668 977 1452 1512
Size: 668 309 475 60
Fragments arranged by size:
668 475 309 60
PpuMI rG'GwC_Cy
Cuts at: 0 589 666 1014 1512
Size: 589 77 348 498
Fragments arranged by size:
589 498 348 77
PspOMI G'GGCC_C
Cuts at: 0 316 679 803 1480 1512
Size: 316 363 124 677 32
Fragments arranged by size:
677 363 316 124 32
PstI C_TGCA'G
Cuts at: 0 452 557 572 708 1512
Size: 452 105 15 136 804
Fragments arranged by size:
804 452 136 105 15
PvuII CAG'CTG
Cuts at: 0 80 852 966 1330 1501 1512
Size: 80 772 114 364 171 11
Fragments arranged by size:
772 364 171 114 80 11
SacI G_AGCT'C
Cuts at: 0 1154 1400 1512
Size: 1154 246 112
Fragments arranged by size:
1154 246 112
SacII CC_GC'GG
Cuts at: 0 987 1512
Size: 987 525
SanDI GG'GwC_CC
Cuts at: 0 589 1512
Size: 589 923
SapI GCTCTTCn'nnn_
Cuts at: 0 437 844 1512
Size: 437 407 668
Fragments arranged by size:
668 437 407
SbfI CC_TGCA'GG
Cuts at: 0 708 1512
Size: 708 804
SfcI C'TryA_G
Cuts at: 0 448 553 568 704 843 1003 1277 1345
Size: 448 105 15 136 139 160 274 68
Cuts at: 1345 1512
Size: 167
Fragments arranged by size:
448 274 167 160 139 136 105 68
15
SmaI CCC'GGG
Cuts at: 0 322 802 1512
Size: 322 480 710
Fragments arranged by size:
710 480 322
SphI G_CATG'C
Cuts at: 0 148 1509 1512
Size: 148 1361 3
Fragments arranged by size:
1361 148 3
StuI AGG'CCT
Cuts at: 0 196 1463 1512
Size: 196 1267 49
Fragments arranged by size:
1267 196 49
StyI C'CwwG_G
Cuts at: 0 203 825 1464 1512
Size: 203 622 639 48
Fragments arranged by size:
639 622 203 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 877 1511 1512
Size: 775 102 634 1
Fragments arranged by size:
775 634 102 1
TatI w'GTAC_w
Cuts at: 0 1263 1512
Size: 1263 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 523 555 1512
Size: 523 32 957
Fragments arranged by size:
957 523 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 98 1450 1512
Size: 98 1352 62
Fragments arranged by size:
1352 98 62
XmaI C'CCGG_G
Cuts at: 0 320 800 1512
Size: 320 480 712
Fragments arranged by size:
712 480 320
XmnI GAAnn'nnTTC
Cuts at: 0 124 1512
Size: 124 1388
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII HincII HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI SfcI SmaI SphI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BfrBI BlpI BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspI FspAI Hin4I
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI