Restriction map for
pF1KB5926
(Linear) (Six-base) MAPSORT of: KIBB5926.seq Check: 7987 from: 1 to: 1608
KIBB5926.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1371 1608
Size: 1371 237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 904 1600 1608
Size: 578 326 696 8
Fragments arranged by size:
696 578 326 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1057 1608
Size: 1057 551
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 562 594 1608
Size: 280 32 250 32 1014
Fragments arranged by size:
1014 280 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 556 879 882 1240 1527 1608
Size: 96 460 323 3 358 287 81
Fragments arranged by size:
460 358 323 287 96 81 3
ApaI G_GGCC'C
Cuts at: 0 1606 1608
Size: 1606 2
AvaI C'yCGr_G
Cuts at: 0 190 1608
Size: 190 1418
BamHI G'GATC_C
Cuts at: 0 1112 1608
Size: 1112 496
BanI G'GyrC_C
Cuts at: 0 48 1608
Size: 48 1560
BanII G_rGCy'C
Cuts at: 0 23 170 341 1606 1608
Size: 23 147 171 1265 2
Fragments arranged by size:
1265 171 147 23 2
BbeI G_GCGC'C
Cuts at: 0 52 1608
Size: 52 1556
BbsI GAAGACnn'nnnn_
Cuts at: 0 892 1608
Size: 892 716
BbvCI CC'TCA_GC
Cuts at: 0 32 1001 1069 1131 1608
Size: 32 969 68 62 477
Fragments arranged by size:
969 477 68 62 32
BglI GCCn_nnn'nGGC
Cuts at: 0 521 1608
Size: 521 1087
BglII A'GATC_T
Cuts at: 0 1319 1608
Size: 1319 289
Bme1580I G_kGCm'C
Cuts at: 0 405 987 1606 1608
Size: 405 582 619 2
Fragments arranged by size:
619 582 405 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 922 1608
Size: 186 736 686
Fragments arranged by size:
736 686 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 262 533 1204 1306 1608
Size: 85 177 271 671 102 302
Fragments arranged by size:
671 302 271 177 102 85
Bpu10I CC'TnA_GC
Cuts at: 0 32 1001 1069 1131 1608
Size: 32 969 68 62 477
Fragments arranged by size:
969 477 68 62 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 690 1024 1510 1608
Size: 690 334 486 98
Fragments arranged by size:
690 486 334 98
BsaHI Gr'CG_yC
Cuts at: 0 49 1608
Size: 49 1559
BsaWI w'CCGG_w
Cuts at: 0 9 1608
Size: 9 1599
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 886 916 1608
Size: 562 30 294 30 692
Fragments arranged by size:
692 562 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 331 580 910 1608
Size: 223 108 249 330 698
Fragments arranged by size:
698 330 249 223 108
BseYI C'CCAG_C
Cuts at: 0 147 161 362 510 581 961 986 1311
Size: 147 14 201 148 71 380 25 325
Cuts at: 1311 1527 1608
Size: 216 81
Fragments arranged by size:
380 325 216 201 148 147 81 71
25 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1017 1608
Size: 241 776 591
Fragments arranged by size:
776 591 241
BsiEI CG_ry'CG
Cuts at: 0 218 237 1608
Size: 218 19 1371
Fragments arranged by size:
1371 218 19
BsiHKAI G_wGCw'C
Cuts at: 0 140 1220 1608
Size: 140 1080 388
Fragments arranged by size:
1080 388 140
Bsp1286I G_dGCh'C
Cuts at: 0 23 140 170 341 405 987 1220 1606
Size: 23 117 30 171 64 582 233 386
Cuts at: 1606 1608
Size: 2
Fragments arranged by size:
582 386 233 171 117 64 30 23
2
BspHI T'CATG_A
Cuts at: 0 684 1608
Size: 684 924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1371 1608
Size: 1371 237
BsrBI CCG'CTC
Cuts at: 0 1377 1608
Size: 1377 231
BsrDI GCAATG_nn'
Cuts at: 0 1080 1608
Size: 1080 528
BsrFI r'CCGG_y
Cuts at: 0 45 213 1608
Size: 45 168 1395
Fragments arranged by size:
1395 168 45
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 879 1608
Size: 96 783 729
Fragments arranged by size:
783 729 96
BstBI TT'CG_AA
Cuts at: 0 796 1608
Size: 796 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 589 969 1458 1608
Size: 589 380 489 150
Fragments arranged by size:
589 489 380 150
BstYI r'GATC_y
Cuts at: 0 1112 1319 1608
Size: 1112 207 289
Fragments arranged by size:
1112 289 207
Bsu36I CC'TnA_GG
Cuts at: 0 110 632 1567 1608
Size: 110 522 935 41
Fragments arranged by size:
935 522 110 41
BtgI C'CryG_G
Cuts at: 0 156 446 1457 1608
Size: 156 290 1011 151
Fragments arranged by size:
1011 290 156 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 305 1608
Size: 305 1303
DrdI GACnn_nn'nnGTC
Cuts at: 0 763 1608
Size: 763 845
EaeI y'GGCC_r
Cuts at: 0 234 308 316 1608
Size: 234 74 8 1292
Fragments arranged by size:
1292 234 74 8
EagI C'GGCC_G
Cuts at: 0 234 1608
Size: 234 1374
EarI CTCTTCn'nnn_
Cuts at: 0 557 611 634 665 1608
Size: 557 54 23 31 943
Fragments arranged by size:
943 557 54 31 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 262 533 578 904 1204 1306 1600
Size: 85 177 271 45 326 300 102 294
Cuts at: 1600 1608
Size: 8
Fragments arranged by size:
326 300 294 271 177 102 85 45
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 1566 1608
Size: 88 1478 42
Fragments arranged by size:
1478 88 42
EcoO109I rG'GnC_Cy
Cuts at: 0 69 106 195 226 369 440 645 1224
Size: 69 37 89 31 143 71 205 579
Cuts at: 1224 1237 1603 1608
Size: 13 366 5
Fragments arranged by size:
579 366 205 143 89 71 69 37
31 13 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1605 1608
Size: 1605 3
HaeII r_GCGC'y
Cuts at: 0 52 97 148 1202 1608
Size: 52 45 51 1054 406
Fragments arranged by size:
1054 406 52 51 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1100 1132 1482 1514 1608
Size: 1100 32 350 32 94
Fragments arranged by size:
1100 350 94 32 32
KasI G'GCGC_C
Cuts at: 0 48 1608
Size: 48 1560
MslI CAynn'nnrTG
Cuts at: 0 1456 1608
Size: 1456 152
MspA1I CmG'CkG
Cuts at: 0 63 158 879 990 1608
Size: 63 95 721 111 618
Fragments arranged by size:
721 618 111 95 63
NaeI GCC'GGC
Cuts at: 0 47 1608
Size: 47 1561
NarI GG'CG_CC
Cuts at: 0 49 1608
Size: 49 1559
NcoI C'CATG_G
Cuts at: 0 446 1457 1608
Size: 446 1011 151
Fragments arranged by size:
1011 446 151
NgoMIV G'CCGG_C
Cuts at: 0 45 1608
Size: 45 1563
PasI CC'CwG_GG
Cuts at: 0 25 53 405 1608
Size: 25 28 352 1203
Fragments arranged by size:
1203 352 28 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 1527 1608
Size: 1527 81
PfoI T'CCnGG_A
Cuts at: 0 851 1608
Size: 851 757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 1608
Size: 562 32 1014
Fragments arranged by size:
1014 562 32
PpuMI rG'GwC_Cy
Cuts at: 0 369 440 645 1237 1608
Size: 369 71 205 592 371
Fragments arranged by size:
592 371 369 205 71
PshAI GACnn'nnGTC
Cuts at: 0 179 236 1016 1608
Size: 179 57 780 592
Fragments arranged by size:
780 592 179 57
PspOMI G'GGCC_C
Cuts at: 0 1602 1608
Size: 1602 6
PspXI vC'TCGA_Gb
Cuts at: 0 190 1608
Size: 190 1418
PstI C_TGCA'G
Cuts at: 0 92 101 356 875 995 1198 1608
Size: 92 9 255 519 120 203 410
Fragments arranged by size:
519 410 255 203 120 92 9
PvuII CAG'CTG
Cuts at: 0 63 879 990 1608
Size: 63 816 111 618
Fragments arranged by size:
816 618 111 63
SacII CC_GC'GG
Cuts at: 0 159 1608
Size: 159 1449
SanDI GG'GwC_CC
Cuts at: 0 645 1608
Size: 645 963
SfcI C'TryA_G
Cuts at: 0 88 97 352 871 937 991 1194 1608
Size: 88 9 255 519 66 54 203 414
Fragments arranged by size:
519 414 255 203 88 66 54 9
SfoI GGC'GCC
Cuts at: 0 50 1608
Size: 50 1558
SmlI C'TyrA_G
Cuts at: 0 190 1608
Size: 190 1418
StyI C'CwwG_G
Cuts at: 0 446 890 1457 1608
Size: 446 444 567 151
Fragments arranged by size:
567 446 444 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1608
Size: 1006 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 964 996 1608
Size: 964 32 612
Fragments arranged by size:
964 612 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1455 1608
Size: 1455 153
XhoI C'TCGA_G
Cuts at: 0 190 1608
Size: 190 1418
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacII SanDI SfcI SfoI
SmlI StyI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BssSI BstEII BstZ17I BtsI ClaI CspCI
DraI DraIII EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PsiI PsrI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XmaI XmnI ZraI