Restriction map for
pF1KB5927
(Linear) (Six-base) MAPSORT of: KIBB5927.seq Check: 303 from: 1 to: 1701
KIBB5927.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 312 1701
Size: 312 1389
AccI GT'mk_AC
Cuts at: 0 298 1409 1701
Size: 298 1111 292
Fragments arranged by size:
1111 298 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1701
Size: 688 1013
AhdI GACnn_n'nnGTC
Cuts at: 0 1690 1701
Size: 1690 11
AleI CACnn'nnGTG
Cuts at: 0 184 1701
Size: 184 1517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 1701
Size: 136 1565
AlwNI CAG_nnn'CTG
Cuts at: 0 368 1701
Size: 368 1333
ApaI G_GGCC'C
Cuts at: 0 744 1701
Size: 744 957
ApoI r'AATT_y
Cuts at: 0 535 553 1270 1701
Size: 535 18 717 431
Fragments arranged by size:
717 535 431 18
AvrII C'CTAG_G
Cuts at: 0 903 1701
Size: 903 798
BamHI G'GATC_C
Cuts at: 0 1314 1584 1701
Size: 1314 270 117
Fragments arranged by size:
1314 270 117
BanII G_rGCy'C
Cuts at: 0 13 744 931 1627 1701
Size: 13 731 187 696 74
Fragments arranged by size:
731 696 187 74 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 785 1429 1701
Size: 534 251 644 272
Fragments arranged by size:
644 534 272 251
BfrBI ATG'CAT
Cuts at: 0 1504 1701
Size: 1504 197
BglI GCCn_nnn'nGGC
Cuts at: 0 914 1701
Size: 914 787
BlpI GC'TnA_GC
Cuts at: 0 1621 1701
Size: 1621 80
Bme1580I G_kGCm'C
Cuts at: 0 35 744 1701
Size: 35 709 957
Fragments arranged by size:
957 709 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 580 1272 1701
Size: 580 692 429
Fragments arranged by size:
692 580 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 1369 1518 1701
Size: 395 974 149 183
Fragments arranged by size:
974 395 183 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 315 1069 1701
Size: 315 754 632
Fragments arranged by size:
754 632 315
BsaAI yAC'GTr
Cuts at: 0 1443 1701
Size: 1443 258
BsaBI GATnn'nnATC
Cuts at: 0 596 1701
Size: 596 1105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 863 1026 1701
Size: 863 163 675
Fragments arranged by size:
863 675 163
BseYI C'CCAG_C
Cuts at: 0 658 735 744 1080 1588 1701
Size: 658 77 9 336 508 113
Fragments arranged by size:
658 508 336 113 77 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 259 413 1701
Size: 162 97 154 1288
Fragments arranged by size:
1288 162 154 97
BsiEI CG_ry'CG
Cuts at: 0 61 65 168 1701
Size: 61 4 103 1533
Fragments arranged by size:
1533 103 61 4
BsmI GAATG_Cn'
Cuts at: 0 971 1701
Size: 971 730
Bsp1286I G_dGCh'C
Cuts at: 0 13 35 744 931 1627 1701
Size: 13 22 709 187 696 74
Fragments arranged by size:
709 696 187 74 22 13
BspHI T'CATG_A
Cuts at: 0 1337 1662 1701
Size: 1337 325 39
Fragments arranged by size:
1337 325 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 167 194 312 1283 1701
Size: 128 39 27 118 971 418
Fragments arranged by size:
971 418 128 118 39 27
BsrDI GCAATG_nn'
Cuts at: 0 692 845 950 1701
Size: 692 153 105 751
Fragments arranged by size:
751 692 153 105
BsrFI r'CCGG_y
Cuts at: 0 60 64 91 1701
Size: 60 4 27 1610
Fragments arranged by size:
1610 60 27 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 1701
Size: 877 824
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 373 1088 1701
Size: 189 184 715 613
Fragments arranged by size:
715 613 189 184
BstYI r'GATC_y
Cuts at: 0 1314 1584 1701
Size: 1314 270 117
Fragments arranged by size:
1314 270 117
Bsu36I CC'TnA_GG
Cuts at: 0 1483 1701
Size: 1483 218
BtgI C'CryG_G
Cuts at: 0 182 248 999 1701
Size: 182 66 751 702
Fragments arranged by size:
751 702 182 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1623 1701
Size: 1623 78
BtsI GCAGTG_nn'
Cuts at: 0 366 1622 1701
Size: 366 1256 79
Fragments arranged by size:
1256 366 79
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1116 1151 1270 1305 1701
Size: 1116 35 119 35 396
Fragments arranged by size:
1116 396 119 35 35
EaeI y'GGCC_r
Cuts at: 0 58 62 221 245 251 944 1046 1275
Size: 58 4 159 24 6 693 102 229
Cuts at: 1275 1380 1593 1674 1701
Size: 105 213 81 27
Fragments arranged by size:
693 229 213 159 105 102 81 58
27 24 6 4
EagI C'GGCC_G
Cuts at: 0 58 62 1701
Size: 58 4 1639
Fragments arranged by size:
1639 58 4
EarI CTCTTCn'nnn_
Cuts at: 0 103 833 1136 1701
Size: 103 730 303 565
Fragments arranged by size:
730 565 303 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 688 1369 1518 1701
Size: 395 293 681 149 183
Fragments arranged by size:
681 395 293 183 149
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1701
Size: 100 1601
EcoO109I rG'GnC_Cy
Cuts at: 0 424 703 740 741 1062 1344 1353 1479
Size: 424 279 37 1 321 282 9 126
Cuts at: 1479 1684 1701
Size: 205 17
Fragments arranged by size:
424 321 282 279 205 126 37 17
9 1
EcoRV GAT'ATC
Cuts at: 0 594 1701
Size: 594 1107
FseI GG_CCGG'CC
Cuts at: 0 64 1701
Size: 64 1637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 1676 1701
Size: 427 32 1217 25
Fragments arranged by size:
1217 427 32 25
HincII GTy'rAC
Cuts at: 0 299 1701
Size: 299 1402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 1701
Size: 1292 409
MscI TGG'CCA
Cuts at: 0 946 1048 1382 1701
Size: 946 102 334 319
Fragments arranged by size:
946 334 319 102
MslI CAynn'nnrTG
Cuts at: 0 19 184 187 1701
Size: 19 165 3 1514
Fragments arranged by size:
1514 165 19 3
MspA1I CmG'CkG
Cuts at: 0 57 250 340 1001 1084 1592 1701
Size: 57 193 90 661 83 508 109
Fragments arranged by size:
661 508 193 109 90 83 57
NaeI GCC'GGC
Cuts at: 0 62 66 1701
Size: 62 4 1635
Fragments arranged by size:
1635 62 4
NcoI C'CATG_G
Cuts at: 0 182 1701
Size: 182 1519
NdeI CA'TA_TG
Cuts at: 0 816 1701
Size: 816 885
NgoMIV G'CCGG_C
Cuts at: 0 60 64 1701
Size: 60 4 1637
Fragments arranged by size:
1637 60 4
NsiI A_TGCA'T
Cuts at: 0 1506 1701
Size: 1506 195
PpuMI rG'GwC_Cy
Cuts at: 0 424 703 1062 1344 1684 1701
Size: 424 279 359 282 340 17
Fragments arranged by size:
424 359 340 282 279 17
PspOMI G'GGCC_C
Cuts at: 0 740 1701
Size: 740 961
PvuII CAG'CTG
Cuts at: 0 340 1084 1701
Size: 340 744 617
Fragments arranged by size:
744 617 340
SacII CC_GC'GG
Cuts at: 0 251 1002 1701
Size: 251 751 699
Fragments arranged by size:
751 699 251
SalI G'TCGA_C
Cuts at: 0 297 1701
Size: 297 1404
SanDI GG'GwC_CC
Cuts at: 0 1684 1701
Size: 1684 17
SfcI C'TryA_G
Cuts at: 0 1410 1701
Size: 1410 291
StyI C'CwwG_G
Cuts at: 0 182 903 1701
Size: 182 721 798
Fragments arranged by size:
798 721 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 1174 1206 1221 1253 1701
Size: 695 32 447 32 15 32 448
Fragments arranged by size:
695 448 447 32 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1032 1701
Size: 231 801 669
Fragments arranged by size:
801 669 231
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI ApaI
ApoI AvrII BamHI BanII BbsI BfrBI BglI BlpI
Bme1580I BmrI BpmI BsaI BsaAI BsaBI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV FseI
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI PpuMI PspOMI PvuII SacII SalI
SanDI SfcI StyI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
ApaLI AscI AseI AsiSI AvaI BaeI BanI BbeI
BbvCI BcgI BciVI BclI BglII BmgBI BmtI BplI
Bpu10I BpuEI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI DraI DraIII DrdI EciI EcoICRI EcoRI FalI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NarI NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI