Restriction map for
pF1KB5930
(Linear) (Six-base) MAPSORT of: KIBB5930.seq Check: 5296 from: 1 to: 1614
KIBB5930.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 247 585 719 1614
Size: 247 338 134 895
Fragments arranged by size:
895 338 247 134
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 445 469 784 1614
Size: 193 252 24 315 830
Fragments arranged by size:
830 315 252 193 24
AflIII A'CryG_T
Cuts at: 0 1463 1614
Size: 1463 151
AlwNI CAG_nnn'CTG
Cuts at: 0 1052 1138 1614
Size: 1052 86 476
Fragments arranged by size:
1052 476 86
ApaI G_GGCC'C
Cuts at: 0 611 1322 1614
Size: 611 711 292
Fragments arranged by size:
711 611 292
ApaLI G'TGCA_C
Cuts at: 0 878 1614
Size: 878 736
AvaI C'yCGr_G
Cuts at: 0 579 1071 1528 1614
Size: 579 492 457 86
Fragments arranged by size:
579 492 457 86
BanI G'GyrC_C
Cuts at: 0 927 1614
Size: 927 687
BanII G_rGCy'C
Cuts at: 0 611 922 973 1081 1229 1322 1438 1489
Size: 611 311 51 108 148 93 116 51
Cuts at: 1489 1597 1614
Size: 108 17
Fragments arranged by size:
611 311 148 116 108 108 93 51
51 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 732 772 1614
Size: 434 298 40 842
Fragments arranged by size:
842 434 298 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 415 449 1614
Size: 415 34 1165
Fragments arranged by size:
1165 415 34
BlpI GC'TnA_GC
Cuts at: 0 1080 1614
Size: 1080 534
Bme1580I G_kGCm'C
Cuts at: 0 611 882 932 1040 1322 1614
Size: 611 271 50 108 282 292
Fragments arranged by size:
611 292 282 271 108 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 1383 1614
Size: 669 714 231
Fragments arranged by size:
714 669 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 204 948 1452 1614
Size: 204 744 504 162
Fragments arranged by size:
744 504 204 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 73 583 660 777 879 922 936
Size: 25 48 510 77 117 102 43 14
Cuts at: 936 961 1243 1430 1614
Size: 25 282 187 184
Fragments arranged by size:
510 282 187 184 117 102 77 48
43 25 25 14
Bpu10I CC'TnA_GC
Cuts at: 0 818 1614
Size: 818 796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1614
Size: 522 1092
BsaI GGTCTCn'nnnn_
Cuts at: 0 485 1422 1614
Size: 485 937 192
Fragments arranged by size:
937 485 192
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 528 544 612 697 712 1108 1614
Size: 528 16 68 85 15 396 506
Fragments arranged by size:
528 506 396 85 68 16 15
BseYI C'CCAG_C
Cuts at: 0 11 50 130 811 906 1045 1197 1228
Size: 11 39 80 681 95 139 152 31
Cuts at: 1228 1378 1614
Size: 150 236
Fragments arranged by size:
681 236 152 150 139 95 80 39
31 11
BsiHKAI G_wGCw'C
Cuts at: 0 882 1614
Size: 882 732
Bsp1286I G_dGCh'C
Cuts at: 0 611 882 922 932 973 1040 1081 1229
Size: 611 271 40 10 41 67 41 148
Cuts at: 1229 1322 1438 1489 1597 1614
Size: 93 116 51 108 17
Fragments arranged by size:
611 271 148 116 108 93 67 51
41 41 40 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 247 585 719 1614
Size: 247 338 134 895
Fragments arranged by size:
895 338 247 134
BsrBI CCG'CTC
Cuts at: 0 822 1614
Size: 822 792
BsrFI r'CCGG_y
Cuts at: 0 357 1400 1447 1614
Size: 357 1043 47 167
Fragments arranged by size:
1043 357 167 47
BsrGI T'GTAC_A
Cuts at: 0 839 947 1614
Size: 839 108 667
Fragments arranged by size:
839 667 108
BstEII G'GTnAC_C
Cuts at: 0 1403 1614
Size: 1403 211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 325 1166 1614
Size: 325 841 448
Fragments arranged by size:
841 448 325
BtgI C'CryG_G
Cuts at: 0 964 1614
Size: 964 650
EaeI y'GGCC_r
Cuts at: 0 266 974 1054 1105 1398 1445 1614
Size: 266 708 80 51 293 47 169
Fragments arranged by size:
708 293 266 169 80 51 47
EarI CTCTTCn'nnn_
Cuts at: 0 161 1614
Size: 161 1453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1549 1571 1614
Size: 1549 22 43
Fragments arranged by size:
1549 43 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 73 193 445 469 583 660 777
Size: 25 48 120 252 24 114 77 117
Cuts at: 777 784 879 922 936 961 1243 1430 1614
Size: 7 95 43 14 25 282 187 184
Fragments arranged by size:
282 252 187 184 120 117 114 95
77 48 43 25 25 24 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 841 1310 1614
Size: 841 469 304
Fragments arranged by size:
841 469 304
EcoO109I rG'GnC_Cy
Cuts at: 0 607 635 910 1245 1318 1319 1540 1614
Size: 607 28 275 335 73 1 221 74
Fragments arranged by size:
607 335 275 221 74 73 28 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1065 1614
Size: 1065 549
FseI GG_CCGG'CC
Cuts at: 0 1451 1614
Size: 1451 163
HaeII r_GCGC'y
Cuts at: 0 417 1614
Size: 417 1197
HincII GTy'rAC
Cuts at: 0 288 1614
Size: 288 1326
HindIII A'AGCT_T
Cuts at: 0 457 1614
Size: 457 1157
MslI CAynn'nnrTG
Cuts at: 0 248 1614
Size: 248 1366
MspA1I CmG'CkG
Cuts at: 0 854 953 1178 1297 1614
Size: 854 99 225 119 317
Fragments arranged by size:
854 317 225 119 99
NaeI GCC'GGC
Cuts at: 0 359 1449 1614
Size: 359 1090 165
Fragments arranged by size:
1090 359 165
NgoMIV G'CCGG_C
Cuts at: 0 357 1447 1614
Size: 357 1090 167
Fragments arranged by size:
1090 357 167
NspI r_CATG'y
Cuts at: 0 229 447 1614
Size: 229 218 1167
Fragments arranged by size:
1167 229 218
PasI CC'CwG_GG
Cuts at: 0 497 1306 1323 1391 1614
Size: 497 809 17 68 223
Fragments arranged by size:
809 497 223 68 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 1052 1569 1614
Size: 264 788 517 45
Fragments arranged by size:
788 517 264 45
PfoI T'CCnGG_A
Cuts at: 0 107 1334 1614
Size: 107 1227 280
Fragments arranged by size:
1227 280 107
PpuMI rG'GwC_Cy
Cuts at: 0 635 910 1245 1540 1614
Size: 635 275 335 295 74
Fragments arranged by size:
635 335 295 275 74
PshAI GACnn'nnGTC
Cuts at: 0 1270 1614
Size: 1270 344
PspOMI G'GGCC_C
Cuts at: 0 607 1318 1614
Size: 607 711 296
Fragments arranged by size:
711 607 296
PspXI vC'TCGA_Gb
Cuts at: 0 579 1614
Size: 579 1035
PstI C_TGCA'G
Cuts at: 0 196 476 596 853 989 1177 1498 1614
Size: 196 280 120 257 136 188 321 116
Fragments arranged by size:
321 280 257 196 188 136 120 116
PvuII CAG'CTG
Cuts at: 0 953 1178 1297 1614
Size: 953 225 119 317
Fragments arranged by size:
953 317 225 119
SanDI GG'GwC_CC
Cuts at: 0 910 1614
Size: 910 704
SapI GCTCTTCn'nnn_
Cuts at: 0 161 1614
Size: 161 1453
SfcI C'TryA_G
Cuts at: 0 192 472 592 849 985 1173 1494 1614
Size: 192 280 120 257 136 188 321 120
Fragments arranged by size:
321 280 257 192 188 136 120 120
SmaI CCC'GGG
Cuts at: 0 1530 1614
Size: 1530 84
SmlI C'TyrA_G
Cuts at: 0 537 579 1614
Size: 537 42 1035
Fragments arranged by size:
1035 537 42
SphI G_CATG'C
Cuts at: 0 229 1614
Size: 229 1385
StuI AGG'CCT
Cuts at: 0 1238 1614
Size: 1238 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 861 865 974 1557 1614
Size: 861 4 109 583 57
Fragments arranged by size:
861 583 109 57 4
TatI w'GTAC_w
Cuts at: 0 476 839 947 1614
Size: 476 363 108 667
Fragments arranged by size:
667 476 363 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 1614
Size: 627 32 955
Fragments arranged by size:
955 627 32
Tth111I GACn'n_nGTC
Cuts at: 0 67 658 675 1614
Size: 67 591 17 939
Fragments arranged by size:
939 591 67 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 1614
Size: 903 711
XhoI C'TCGA_G
Cuts at: 0 579 1614
Size: 579 1035
XmaI C'CCGG_G
Cuts at: 0 1528 1614
Size: 1528 86
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BcgI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstEII BstXI BtgI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FseI HaeII HincII
HindIII MslI MspA1I NaeI NgoMIV NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI
SapI SfcI SmaI SmlI SphI StuI TaqII TatI
TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FspI FspAI Hin4I HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StyI SwaI XbaI XmnI ZraI