Restriction map for
pF1KB5935
(Linear) (Six-base) MAPSORT of: KIBB5935.seq Check: 661 from: 1 to: 1524
KIBB5935.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 758 1524
Size: 512 246 766
Fragments arranged by size:
766 512 246
AflIII A'CryG_T
Cuts at: 0 296 1524
Size: 296 1228
AleI CACnn'nnGTG
Cuts at: 0 631 1524
Size: 631 893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 1524
Size: 649 875
ApoI r'AATT_y
Cuts at: 0 748 1449 1524
Size: 748 701 75
Fragments arranged by size:
748 701 75
AseI AT'TA_AT
Cuts at: 0 71 1524
Size: 71 1453
BamHI G'GATC_C
Cuts at: 0 714 1524
Size: 714 810
BbvCI CC'TCA_GC
Cuts at: 0 566 1524
Size: 566 958
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1417 1451 1524
Size: 1417 34 73
Fragments arranged by size:
1417 73 34
BfrBI ATG'CAT
Cuts at: 0 1167 1524
Size: 1167 357
Bme1580I G_kGCm'C
Cuts at: 0 276 1524
Size: 276 1248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 823 1524
Size: 398 425 701
Fragments arranged by size:
701 425 398
Bpu10I CC'TnA_GC
Cuts at: 0 566 1524
Size: 566 958
BsaBI GATnn'nnATC
Cuts at: 0 47 1423 1524
Size: 47 1376 101
Fragments arranged by size:
1376 101 47
BsaHI Gr'CG_yC
Cuts at: 0 1233 1275 1524
Size: 1233 42 249
Fragments arranged by size:
1233 249 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 954 984 1524
Size: 954 30 540
Fragments arranged by size:
954 540 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1524
Size: 299 1225
BsiHKAI G_wGCw'C
Cuts at: 0 519 1212 1524
Size: 519 693 312
Fragments arranged by size:
693 519 312
Bsp1286I G_dGCh'C
Cuts at: 0 276 519 1212 1524
Size: 276 243 693 312
Fragments arranged by size:
693 312 276 243
BsrBI CCG'CTC
Cuts at: 0 615 1524
Size: 615 909
BsrGI T'GTAC_A
Cuts at: 0 1243 1524
Size: 1243 281
BssSI C'ACGA_G
Cuts at: 0 1469 1524
Size: 1469 55
BstYI r'GATC_y
Cuts at: 0 714 1158 1524
Size: 714 444 366
Fragments arranged by size:
714 444 366
Bsu36I CC'TnA_GG
Cuts at: 0 951 1524
Size: 951 573
BtsI GCAGTG_nn'
Cuts at: 0 1338 1462 1524
Size: 1338 124 62
Fragments arranged by size:
1338 124 62
EarI CTCTTCn'nnn_
Cuts at: 0 335 605 810 879 1524
Size: 335 270 205 69 645
Fragments arranged by size:
645 335 270 205 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 512 758 823 1524
Size: 398 114 246 65 701
Fragments arranged by size:
701 398 246 114 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 530 1524
Size: 530 994
EcoRV GAT'ATC
Cuts at: 0 448 457 1269 1524
Size: 448 9 812 255
Fragments arranged by size:
812 448 255 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 463 495 880 912 954 986
Size: 239 32 192 32 385 32 42 32
Cuts at: 986 1116 1148 1468 1500 1524
Size: 130 32 320 32 24
Fragments arranged by size:
385 320 239 192 130 42 32 32
32 32 32 32 24
HincII GTy'rAC
Cuts at: 0 591 903 1317 1524
Size: 591 312 414 207
Fragments arranged by size:
591 414 312 207
HpaI GTT'AAC
Cuts at: 0 903 1524
Size: 903 621
MslI CAynn'nnrTG
Cuts at: 0 73 584 631 1172 1524
Size: 73 511 47 541 352
Fragments arranged by size:
541 511 352 73 47
NsiI A_TGCA'T
Cuts at: 0 1169 1524
Size: 1169 355
NspI r_CATG'y
Cuts at: 0 300 640 1524
Size: 300 340 884
Fragments arranged by size:
884 340 300
PciI A'CATG_T
Cuts at: 0 296 1524
Size: 296 1228
PfoI T'CCnGG_A
Cuts at: 0 414 1524
Size: 414 1110
SapI GCTCTTCn'nnn_
Cuts at: 0 335 605 1524
Size: 335 270 919
Fragments arranged by size:
919 335 270
ScaI AGT'ACT
Cuts at: 0 1509 1524
Size: 1509 15
SfcI C'TryA_G
Cuts at: 0 596 1524
Size: 596 928
SspI AAT'ATT
Cuts at: 0 1225 1524
Size: 1225 299
TatI w'GTAC_w
Cuts at: 0 576 1243 1507 1524
Size: 576 667 264 17
Fragments arranged by size:
667 576 264 17
Tth111I GACn'n_nGTC
Cuts at: 0 34 1524
Size: 34 1490
XbaI T'CTAG_A
Cuts at: 0 1155 1524
Size: 1155 369
Enzymes that do cut:
AcuI AflIII AleI AlfI ApoI AseI BamHI BbvCI
BcgI BfrBI Bme1580I BpmI Bpu10I BsaBI BsaHI BsaXI
BsgI BsiHKAI Bsp1286I BsrBI BsrGI BssSI BstYI Bsu36I
BtsI EarI Eco57MI EcoNI EcoRV Hin4I HincII HpaI
MslI NsiI NspI PciI PfoI SapI ScaI SfcI
SspI TatI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaWI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII TstI XcmI XhoI XmaI XmnI ZraI