Restriction map for
pF1KB5939
(Linear) (Six-base) MAPSORT of: KIBB5939.seq Check: 500 from: 1 to: 1524
KIBB5939.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 297 406 940 1524
Size: 229 68 109 534 584
Fragments arranged by size:
584 534 229 109 68
AflIII A'CryG_T
Cuts at: 0 572 1524
Size: 572 952
AhdI GACnn_n'nnGTC
Cuts at: 0 492 1524
Size: 492 1032
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1524
Size: 1077 447
ApaI G_GGCC'C
Cuts at: 0 403 1524
Size: 403 1121
ApaLI G'TGCA_C
Cuts at: 0 1309 1524
Size: 1309 215
ApoI r'AATT_y
Cuts at: 0 1402 1524
Size: 1402 122
AvaI C'yCGr_G
Cuts at: 0 58 904 1450 1524
Size: 58 846 546 74
Fragments arranged by size:
846 546 74 58
BanII G_rGCy'C
Cuts at: 0 403 1524
Size: 403 1121
BbsI GAAGACnn'nnnn_
Cuts at: 0 1139 1496 1524
Size: 1139 357 28
Fragments arranged by size:
1139 357 28
BclI T'GATC_A
Cuts at: 0 1497 1524
Size: 1497 27
Bme1580I G_kGCm'C
Cuts at: 0 403 937 1313 1524
Size: 403 534 376 211
Fragments arranged by size:
534 403 376 211
BmgBI CAC'GTC
Cuts at: 0 75 489 573 1137 1524
Size: 75 414 84 564 387
Fragments arranged by size:
564 414 387 84 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 1098 1237 1524
Size: 1098 139 287
Fragments arranged by size:
1098 287 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 516 1524
Size: 516 1008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1060 1429 1524
Size: 979 81 369 95
Fragments arranged by size:
979 369 95 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1524
Size: 1312 212
BsaBI GATnn'nnATC
Cuts at: 0 1445 1524
Size: 1445 79
BsaHI Gr'CG_yC
Cuts at: 0 53 248 1524
Size: 53 195 1276
Fragments arranged by size:
1276 195 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 1524
Size: 562 30 932
Fragments arranged by size:
932 562 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 79 271 976 979 1009 1486 1524
Size: 76 3 192 705 3 30 477 38
Fragments arranged by size:
705 477 192 76 38 30 3 3
BseYI C'CCAG_C
Cuts at: 0 199 272 1524
Size: 199 73 1252
Fragments arranged by size:
1252 199 73
BsiEI CG_ry'CG
Cuts at: 0 941 1524
Size: 941 583
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1524
Size: 1313 211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1228 1524
Size: 1228 296
Bsp1286I G_dGCh'C
Cuts at: 0 403 937 1313 1524
Size: 403 534 376 211
Fragments arranged by size:
534 403 376 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 680 1524
Size: 137 543 844
Fragments arranged by size:
844 543 137
BsrBI CCG'CTC
Cuts at: 0 1415 1524
Size: 1415 109
BssHII G'CGCG_C
Cuts at: 0 45 1524
Size: 45 1479
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 1524
Size: 516 1008
BstEII G'GTnAC_C
Cuts at: 0 657 1524
Size: 657 867
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1293 1524
Size: 462 831 231
Fragments arranged by size:
831 462 231
BstYI r'GATC_y
Cuts at: 0 854 1524
Size: 854 670
BtgI C'CryG_G
Cuts at: 0 29 251 1183 1524
Size: 29 222 932 341
Fragments arranged by size:
932 341 222 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 1524
Size: 1248 276
BtsI GCAGTG_nn'
Cuts at: 0 162 1194 1524
Size: 162 1032 330
Fragments arranged by size:
1032 330 162
ClaI AT'CG_AT
Cuts at: 0 536 1524
Size: 536 988
DrdI GACnn_nn'nnGTC
Cuts at: 0 1357 1524
Size: 1357 167
EaeI y'GGCC_r
Cuts at: 0 26 32 159 236 938 1419 1524
Size: 26 6 127 77 702 481 105
Fragments arranged by size:
702 481 127 105 77 26 6
EagI C'GGCC_G
Cuts at: 0 938 1524
Size: 938 586
EarI CTCTTCn'nnn_
Cuts at: 0 923 1524
Size: 923 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 297 406 940 979 1060 1429 1524
Size: 229 68 109 534 39 81 369 95
Fragments arranged by size:
534 369 229 109 95 81 68 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 961 1524
Size: 922 39 563
Fragments arranged by size:
922 563 39
EcoO109I rG'GnC_Cy
Cuts at: 0 1455 1524
Size: 1455 69
HaeII r_GCGC'y
Cuts at: 0 112 1524
Size: 112 1412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 562 594 740 772 1524
Size: 478 32 52 32 146 32 752
Fragments arranged by size:
752 478 146 52 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 587 1524
Size: 476 111 937
Fragments arranged by size:
937 476 111
MscI TGG'CCA
Cuts at: 0 161 238 1421 1524
Size: 161 77 1183 103
Fragments arranged by size:
1183 161 103 77
MspA1I CmG'CkG
Cuts at: 0 199 352 433 699 1524
Size: 199 153 81 266 825
Fragments arranged by size:
825 266 199 153 81
NcoI C'CATG_G
Cuts at: 0 1183 1524
Size: 1183 341
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 1189 1524
Size: 78 1111 335
Fragments arranged by size:
1111 335 78
PfoI T'CCnGG_A
Cuts at: 0 1037 1406 1524
Size: 1037 369 118
Fragments arranged by size:
1037 369 118
PpuMI rG'GwC_Cy
Cuts at: 0 1455 1524
Size: 1455 69
PspOMI G'GGCC_C
Cuts at: 0 399 1524
Size: 399 1125
PspXI vC'TCGA_Gb
Cuts at: 0 904 1524
Size: 904 620
PstI C_TGCA'G
Cuts at: 0 438 1524
Size: 438 1086
PvuII CAG'CTG
Cuts at: 0 352 433 699 1524
Size: 352 81 266 825
Fragments arranged by size:
825 352 266 81
SfcI C'TryA_G
Cuts at: 0 434 1524
Size: 434 1090
SmlI C'TyrA_G
Cuts at: 0 904 1327 1450 1524
Size: 904 423 123 74
Fragments arranged by size:
904 423 123 74
StyI C'CwwG_G
Cuts at: 0 151 239 338 443 662 1183 1524
Size: 151 88 99 105 219 521 341
Fragments arranged by size:
521 341 219 151 105 99 88
TatI w'GTAC_w
Cuts at: 0 977 1524
Size: 977 547
Tth111I GACn'n_nGTC
Cuts at: 0 73 1524
Size: 73 1451
XhoI C'TCGA_G
Cuts at: 0 904 1450 1524
Size: 904 546 74
Fragments arranged by size:
904 546 74
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI
BanII BbsI BclI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BssHII BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MspA1I NcoI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII SfcI SmlI StyI TatI Tth111I XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmtI Bpu10I BsaI BsaAI BsaWI BsgI
BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssSI
BstBI BstZ17I Bsu36I CspCI DraI DraIII EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
XbaI XcmI XmaI XmnI ZraI