Restriction map for
pF1KB5940
(Linear) (Six-base) MAPSORT of: KIBB5940.seq Check: 5333 from: 1 to: 1620
KIBB5940.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 1620
Size: 470 1150
AccI GT'mk_AC
Cuts at: 0 335 1620
Size: 335 1285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1199 1620
Size: 436 763 421
Fragments arranged by size:
763 436 421
AfeI AGC'GCT
Cuts at: 0 146 1620
Size: 146 1474
AflII C'TTAA_G
Cuts at: 0 1018 1620
Size: 1018 602
AgeI A'CCGG_T
Cuts at: 0 1301 1620
Size: 1301 319
AleI CACnn'nnGTG
Cuts at: 0 473 1620
Size: 473 1147
ApaI G_GGCC'C
Cuts at: 0 947 1620
Size: 947 673
ApoI r'AATT_y
Cuts at: 0 306 707 758 844 1119 1328 1620
Size: 306 401 51 86 275 209 292
Fragments arranged by size:
401 306 292 275 209 86 51
AvaI C'yCGr_G
Cuts at: 0 50 389 947 1620
Size: 50 339 558 673
Fragments arranged by size:
673 558 339 50
BanI G'GyrC_C
Cuts at: 0 117 1620
Size: 117 1503
BanII G_rGCy'C
Cuts at: 0 947 1279 1620
Size: 947 332 341
Fragments arranged by size:
947 341 332
BbeI G_GCGC'C
Cuts at: 0 121 1620
Size: 121 1499
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1620
Size: 500 1120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 996 1030 1620
Size: 996 34 590
Fragments arranged by size:
996 590 34
BfrBI ATG'CAT
Cuts at: 0 1053 1620
Size: 1053 567
BglII A'GATC_T
Cuts at: 0 204 778 1533 1620
Size: 204 574 755 87
Fragments arranged by size:
755 574 204 87
BlpI GC'TnA_GC
Cuts at: 0 1346 1620
Size: 1346 274
Bme1580I G_kGCm'C
Cuts at: 0 947 1246 1620
Size: 947 299 374
Fragments arranged by size:
947 374 299
BmgBI CAC'GTC
Cuts at: 0 992 1620
Size: 992 628
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 537 1620
Size: 368 169 1083
Fragments arranged by size:
1083 368 169
Bpu10I CC'TnA_GC
Cuts at: 0 248 1620
Size: 248 1372
BsaI GGTCTCn'nnnn_
Cuts at: 0 944 1620
Size: 944 676
BsaHI Gr'CG_yC
Cuts at: 0 104 118 1620
Size: 104 14 1502
Fragments arranged by size:
1502 104 14
BsaWI w'CCGG_w
Cuts at: 0 159 216 1301 1620
Size: 159 57 1085 319
Fragments arranged by size:
1085 319 159 57
BseYI C'CCAG_C
Cuts at: 0 995 1307 1620
Size: 995 312 313
Fragments arranged by size:
995 313 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 989 1620
Size: 118 871 631
Fragments arranged by size:
871 631 118
BsiHKAI G_wGCw'C
Cuts at: 0 461 900 1279 1620
Size: 461 439 379 341
Fragments arranged by size:
461 439 379 341
Bsp1286I G_dGCh'C
Cuts at: 0 461 900 947 1246 1279 1620
Size: 461 439 47 299 33 341
Fragments arranged by size:
461 439 341 299 47 33
BspEI T'CCGG_A
Cuts at: 0 159 216 1620
Size: 159 57 1404
Fragments arranged by size:
1404 159 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1620
Size: 470 1150
BsrDI GCAATG_nn'
Cuts at: 0 1139 1620
Size: 1139 481
BsrFI r'CCGG_y
Cuts at: 0 120 1301 1620
Size: 120 1181 319
Fragments arranged by size:
1181 319 120
BstEII G'GTnAC_C
Cuts at: 0 999 1620
Size: 999 621
BstXI CCAn_nnnn'nTGG
Cuts at: 0 79 1290 1620
Size: 79 1211 330
Fragments arranged by size:
1211 330 79
BstYI r'GATC_y
Cuts at: 0 204 778 1533 1620
Size: 204 574 755 87
Fragments arranged by size:
755 574 204 87
BstZ17I GTA'TAC
Cuts at: 0 336 1620
Size: 336 1284
BtgI C'CryG_G
Cuts at: 0 171 820 1312 1620
Size: 171 649 492 308
Fragments arranged by size:
649 492 308 171
BtsI GCAGTG_nn'
Cuts at: 0 1259 1620
Size: 1259 361
DraI TTT'AAA
Cuts at: 0 591 1620
Size: 591 1029
EaeI y'GGCC_r
Cuts at: 0 65 1515 1620
Size: 65 1450 105
Fragments arranged by size:
1450 105 65
EarI CTCTTCn'nnn_
Cuts at: 0 971 1272 1620
Size: 971 301 348
Fragments arranged by size:
971 348 301
EcoICRI GAG'CTC
Cuts at: 0 1277 1620
Size: 1277 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1199 1620
Size: 436 763 421
Fragments arranged by size:
763 436 421
EcoNI CCTnn'n_nnAGG
Cuts at: 0 470 1620
Size: 470 1150
EcoO109I rG'GnC_Cy
Cuts at: 0 167 943 944 1620
Size: 167 776 1 676
Fragments arranged by size:
776 676 167 1
EcoRI G'AATT_C
Cuts at: 0 1119 1620
Size: 1119 501
HaeII r_GCGC'y
Cuts at: 0 32 86 121 148 1620
Size: 32 54 35 27 1472
Fragments arranged by size:
1472 54 35 32 27
KasI G'GCGC_C
Cuts at: 0 117 1620
Size: 117 1503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1620
Size: 548 1072
MscI TGG'CCA
Cuts at: 0 1517 1620
Size: 1517 103
MslI CAynn'nnrTG
Cuts at: 0 473 1288 1601 1620
Size: 473 815 313 19
Fragments arranged by size:
815 473 313 19
MspA1I CmG'CkG
Cuts at: 0 63 173 405 443 822 1035 1545 1620
Size: 63 110 232 38 379 213 510 75
Fragments arranged by size:
510 379 232 213 110 75 63 38
NaeI GCC'GGC
Cuts at: 0 122 1620
Size: 122 1498
NarI GG'CG_CC
Cuts at: 0 118 1620
Size: 118 1502
NcoI C'CATG_G
Cuts at: 0 1312 1620
Size: 1312 308
NdeI CA'TA_TG
Cuts at: 0 620 1620
Size: 620 1000
NgoMIV G'CCGG_C
Cuts at: 0 120 1620
Size: 120 1500
NsiI A_TGCA'T
Cuts at: 0 1055 1620
Size: 1055 565
NspI r_CATG'y
Cuts at: 0 727 1032 1620
Size: 727 305 588
Fragments arranged by size:
727 588 305
PasI CC'CwG_GG
Cuts at: 0 90 1620
Size: 90 1530
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 504 1506 1620
Size: 114 390 1002 114
Fragments arranged by size:
1002 390 114 114
PpuMI rG'GwC_Cy
Cuts at: 0 167 1620
Size: 167 1453
PspOMI G'GGCC_C
Cuts at: 0 943 1620
Size: 943 677
PstI C_TGCA'G
Cuts at: 0 186 1081 1620
Size: 186 895 539
Fragments arranged by size:
895 539 186
PvuII CAG'CTG
Cuts at: 0 405 443 1035 1545 1620
Size: 405 38 592 510 75
Fragments arranged by size:
592 510 405 75 38
SacI G_AGCT'C
Cuts at: 0 1279 1620
Size: 1279 341
SacII CC_GC'GG
Cuts at: 0 174 823 1620
Size: 174 649 797
Fragments arranged by size:
797 649 174
SanDI GG'GwC_CC
Cuts at: 0 167 1620
Size: 167 1453
SapI GCTCTTCn'nnn_
Cuts at: 0 971 1620
Size: 971 649
SfcI C'TryA_G
Cuts at: 0 182 1077 1620
Size: 182 895 543
Fragments arranged by size:
895 543 182
SfoI GGC'GCC
Cuts at: 0 119 1620
Size: 119 1501
SmaI CCC'GGG
Cuts at: 0 52 1620
Size: 52 1568
SmlI C'TyrA_G
Cuts at: 0 389 1018 1620
Size: 389 629 602
Fragments arranged by size:
629 602 389
SphI G_CATG'C
Cuts at: 0 1032 1620
Size: 1032 588
StyI C'CwwG_G
Cuts at: 0 1312 1620
Size: 1312 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 987 1620
Size: 987 633
XbaI T'CTAG_A
Cuts at: 0 1333 1620
Size: 1333 287
XhoI C'TCGA_G
Cuts at: 0 389 1620
Size: 389 1231
XmaI C'CCGG_G
Cuts at: 0 50 1620
Size: 50 1570
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AgeI AleI ApaI
ApoI AvaI BanI BanII BbeI BbsI BcgI BfrBI
BglII BlpI Bme1580I BmgBI BmrI Bpu10I BsaI BsaHI
BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrFI BstEII BstXI BstYI BstZ17I BtgI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SfcI SfoI
SmaI SmlI SphI StyI TaqII XbaI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AhdI AlfI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BglI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI NheI NotI NruI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
Tth111I XcmI XmnI ZraI