Restriction map for
pF1KB5952
(Linear) (Six-base) MAPSORT of: KIBB5952.seq Check: 8818 from: 1 to: 1641
KIBB5952.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AatII G_ACGT'C
Cuts at: 0 202 1641
Size: 202 1439
AccI GT'mk_AC
Cuts at: 0 761 1641
Size: 761 880
Acc65I G'GTAC_C
Cuts at: 0 12 1641
Size: 12 1629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1512 1641
Size: 1063 449 129
Fragments arranged by size:
1063 449 129
AflIII A'CryG_T
Cuts at: 0 1254 1641
Size: 1254 387
AleI CACnn'nnGTG
Cuts at: 0 454 1641
Size: 454 1187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1597 1641
Size: 1597 44
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1641
Size: 1135 32 474
Fragments arranged by size:
1135 474 32
AlwNI CAG_nnn'CTG
Cuts at: 0 38 600 786 1641
Size: 38 562 186 855
Fragments arranged by size:
855 562 186 38
AvaI C'yCGr_G
Cuts at: 0 866 914 1019 1059 1135 1291 1641
Size: 866 48 105 40 76 156 350
Fragments arranged by size:
866 350 156 105 76 48 40
AvrII C'CTAG_G
Cuts at: 0 891 942 1641
Size: 891 51 699
Fragments arranged by size:
891 699 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 29 1641
Size: 29 1612
BamHI G'GATC_C
Cuts at: 0 278 1340 1641
Size: 278 1062 301
Fragments arranged by size:
1062 301 278
BanI G'GyrC_C
Cuts at: 0 12 366 862 1329 1641
Size: 12 354 496 467 312
Fragments arranged by size:
496 467 354 312 12
BanII G_rGCy'C
Cuts at: 0 113 558 971 1020 1450 1641
Size: 113 445 413 49 430 191
Fragments arranged by size:
445 430 413 191 113 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 1458 1518 1641
Size: 1458 60 123
Fragments arranged by size:
1458 123 60
BbvCI CC'TCA_GC
Cuts at: 0 621 961 1641
Size: 621 340 680
Fragments arranged by size:
680 621 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1199 1233 1641
Size: 1199 34 408
Fragments arranged by size:
1199 408 34
BglI GCCn_nnn'nGGC
Cuts at: 0 381 597 751 1641
Size: 381 216 154 890
Fragments arranged by size:
890 381 216 154
BlpI GC'TnA_GC
Cuts at: 0 481 1641
Size: 481 1160
Bme1580I G_kGCm'C
Cuts at: 0 867 1641
Size: 867 774
BmgBI CAC'GTC
Cuts at: 0 1257 1592 1641
Size: 1257 335 49
Fragments arranged by size:
1257 335 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1302 1641
Size: 155 1147 339
Fragments arranged by size:
1147 339 155
Bpu10I CC'TnA_GC
Cuts at: 0 621 961 1641
Size: 621 340 680
Fragments arranged by size:
680 621 340
BsaHI Gr'CG_yC
Cuts at: 0 199 1641
Size: 199 1442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1536 1566 1641
Size: 415 30 1091 30 75
Fragments arranged by size:
1091 415 75 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 312 487 490 517 1582 1641
Size: 312 175 3 27 1065 59
Fragments arranged by size:
1065 312 175 59 27 3
BseYI C'CCAG_C
Cuts at: 0 172 236 467 600 771 1012 1223 1441
Size: 172 64 231 133 171 241 211 218
Cuts at: 1441 1641
Size: 200
Fragments arranged by size:
241 231 218 211 200 172 171 133
64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 1641
Size: 705 936
BsiEI CG_ry'CG
Cuts at: 0 1052 1641
Size: 1052 589
BsiHKAI G_wGCw'C
Cuts at: 0 128 1450 1538 1576 1641
Size: 128 1322 88 38 65
Fragments arranged by size:
1322 128 88 65 38
BsmI GAATG_Cn'
Cuts at: 0 795 1641
Size: 795 846
BsmBI CGTCTCn'nnnn_
Cuts at: 0 190 536 897 1641
Size: 190 346 361 744
Fragments arranged by size:
744 361 346 190
Bsp1286I G_dGCh'C
Cuts at: 0 113 128 558 867 971 1020 1450 1538
Size: 113 15 430 309 104 49 430 88
Cuts at: 1538 1576 1641
Size: 38 65
Fragments arranged by size:
430 430 309 113 104 88 65 49
38 15
BspHI T'CATG_A
Cuts at: 0 800 1641
Size: 800 841
BsrBI CCG'CTC
Cuts at: 0 1547 1641
Size: 1547 94
BsrDI GCAATG_nn'
Cuts at: 0 306 1619 1641
Size: 306 1313 22
Fragments arranged by size:
1313 306 22
BsrFI r'CCGG_y
Cuts at: 0 387 485 711 1332 1641
Size: 387 98 226 621 309
Fragments arranged by size:
621 387 309 226 98
BsrGI T'GTAC_A
Cuts at: 0 338 1641
Size: 338 1303
BssHII G'CGCG_C
Cuts at: 0 844 846 1641
Size: 844 2 795
Fragments arranged by size:
844 795 2
BssSI C'ACGA_G
Cuts at: 0 673 1400 1641
Size: 673 727 241
Fragments arranged by size:
727 673 241
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 531 1641
Size: 231 300 1110
Fragments arranged by size:
1110 300 231
BstYI r'GATC_y
Cuts at: 0 278 1340 1641
Size: 278 1062 301
Fragments arranged by size:
1062 301 278
BtgI C'CryG_G
Cuts at: 0 8 549 807 839 1052 1641
Size: 8 541 258 32 213 589
Fragments arranged by size:
589 541 258 213 32 8
BtsI GCAGTG_nn'
Cuts at: 0 503 518 1131 1270 1641
Size: 503 15 613 139 371
Fragments arranged by size:
613 503 371 139 15
EaeI y'GGCC_r
Cuts at: 0 2 221 533 709 824 1049 1641
Size: 2 219 312 176 115 225 592
Fragments arranged by size:
592 312 225 219 176 115 2
EagI C'GGCC_G
Cuts at: 0 1049 1641
Size: 1049 592
EcoICRI GAG'CTC
Cuts at: 0 1448 1641
Size: 1448 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1063 1302 1512 1641
Size: 155 908 239 210 129
Fragments arranged by size:
908 239 210 155 129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 1641
Size: 208 1433
EcoO109I rG'GnC_Cy
Cuts at: 0 35 140 946 1641
Size: 35 105 806 695
Fragments arranged by size:
806 695 105 35
HaeII r_GCGC'y
Cuts at: 0 772 1641
Size: 772 869
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 1013 1045 1563 1595 1641
Size: 415 32 566 32 518 32 46
Fragments arranged by size:
566 518 415 46 32 32 32
HincII GTy'rAC
Cuts at: 0 1356 1641
Size: 1356 285
KpnI G_GTAC'C
Cuts at: 0 16 1641
Size: 16 1625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1641
Size: 256 1385
MscI TGG'CCA
Cuts at: 0 4 223 535 1641
Size: 4 219 312 1106
Fragments arranged by size:
1106 312 219 4
MslI CAynn'nnrTG
Cuts at: 0 334 454 1594 1641
Size: 334 120 1140 47
Fragments arranged by size:
1140 334 120 47
MspA1I CmG'CkG
Cuts at: 0 511 1054 1179 1641
Size: 511 543 125 462
Fragments arranged by size:
543 511 462 125
NaeI GCC'GGC
Cuts at: 0 487 1641
Size: 487 1154
NcoI C'CATG_G
Cuts at: 0 8 1641
Size: 8 1633
NgoMIV G'CCGG_C
Cuts at: 0 485 1641
Size: 485 1156
NspI r_CATG'y
Cuts at: 0 588 1220 1641
Size: 588 632 421
Fragments arranged by size:
632 588 421
PasI CC'CwG_GG
Cuts at: 0 32 594 1488 1641
Size: 32 562 894 153
Fragments arranged by size:
894 562 153 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1641
Size: 600 1041
PfoI T'CCnGG_A
Cuts at: 0 347 1641
Size: 347 1294
PpuMI rG'GwC_Cy
Cuts at: 0 140 946 1641
Size: 140 806 695
Fragments arranged by size:
806 695 140
PspXI vC'TCGA_Gb
Cuts at: 0 1019 1641
Size: 1019 622
PstI C_TGCA'G
Cuts at: 0 160 1126 1265 1641
Size: 160 966 139 376
Fragments arranged by size:
966 376 160 139
PvuII CAG'CTG
Cuts at: 0 1179 1641
Size: 1179 462
SacI G_AGCT'C
Cuts at: 0 1450 1641
Size: 1450 191
SacII CC_GC'GG
Cuts at: 0 1055 1641
Size: 1055 586
SanDI GG'GwC_CC
Cuts at: 0 140 1641
Size: 140 1501
ScaI AGT'ACT
Cuts at: 0 162 1641
Size: 162 1479
SfcI C'TryA_G
Cuts at: 0 156 1122 1261 1641
Size: 156 966 139 380
Fragments arranged by size:
966 380 156 139
SmaI CCC'GGG
Cuts at: 0 868 916 1061 1641
Size: 868 48 145 580
Fragments arranged by size:
868 580 145 48
SmlI C'TyrA_G
Cuts at: 0 1019 1135 1641
Size: 1019 116 506
Fragments arranged by size:
1019 506 116
StyI C'CwwG_G
Cuts at: 0 8 191 379 891 942 1641
Size: 8 183 188 512 51 699
Fragments arranged by size:
699 512 188 183 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1223 1641
Size: 1223 418
TatI w'GTAC_w
Cuts at: 0 160 293 338 1641
Size: 160 133 45 1303
Fragments arranged by size:
1303 160 133 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 691 723 938 970 1641
Size: 596 32 63 32 215 32 671
Fragments arranged by size:
671 596 215 63 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 23 424 450 1137 1641
Size: 23 401 26 687 504
Fragments arranged by size:
687 504 401 26 23
XhoI C'TCGA_G
Cuts at: 0 1019 1135 1641
Size: 1019 116 506
Fragments arranged by size:
1019 506 116
XmaI C'CCGG_G
Cuts at: 0 866 914 1059 1641
Size: 866 48 145 582
Fragments arranged by size:
866 582 145 48
ZraI GAC'GTC
Cuts at: 0 200 1641
Size: 200 1441
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstXI BstYI BtgI BtsI EaeI EagI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PspXI PstI PvuII SacI SacII
SanDI ScaI SfcI SmaI SmlI StyI TaqII TatI
TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI ApaI ApaLI
ApoI AscI AseI AsiSI BbeI BciVI BclI BfrBI
BglII BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsiWI BspEI BspMI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI NarI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XmnI