Restriction map for
pF1KB5954
(Linear) (Six-base) MAPSORT of: KIBB5954.seq Check: 174 from: 1 to: 1644
KIBB5954.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1113 1644
Size: 1113 531
AccI GT'mk_AC
Cuts at: 0 1385 1504 1644
Size: 1385 119 140
Fragments arranged by size:
1385 140 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1644
Size: 451 1193
AflIII A'CryG_T
Cuts at: 0 722 1388 1528 1604 1644
Size: 722 666 140 76 40
Fragments arranged by size:
722 666 140 76 40
AgeI A'CCGG_T
Cuts at: 0 1055 1644
Size: 1055 589
AleI CACnn'nnGTG
Cuts at: 0 1009 1644
Size: 1009 635
ApoI r'AATT_y
Cuts at: 0 426 1240 1644
Size: 426 814 404
Fragments arranged by size:
814 426 404
AseI AT'TA_AT
Cuts at: 0 1292 1644
Size: 1292 352
AvaI C'yCGr_G
Cuts at: 0 236 1644
Size: 236 1408
BanI G'GyrC_C
Cuts at: 0 841 989 1644
Size: 841 148 655
Fragments arranged by size:
841 655 148
BanII G_rGCy'C
Cuts at: 0 1130 1644
Size: 1130 514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1589 1623 1644
Size: 1589 34 21
Fragments arranged by size:
1589 34 21
BclI T'GATC_A
Cuts at: 0 656 1644
Size: 656 988
BmgBI CAC'GTC
Cuts at: 0 1389 1605 1644
Size: 1389 216 39
Fragments arranged by size:
1389 216 39
BmtI G_CTAG'C
Cuts at: 0 10 1644
Size: 10 1634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 659 1644
Size: 566 93 985
Fragments arranged by size:
985 566 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1644
Size: 907 737
BsaAI yAC'GTr
Cuts at: 0 135 1011 1644
Size: 135 876 633
Fragments arranged by size:
876 633 135
BsaWI w'CCGG_w
Cuts at: 0 1055 1644
Size: 1055 589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1318 1348 1644
Size: 1318 30 296
Fragments arranged by size:
1318 296 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 656 679 728 797 1644
Size: 656 23 49 69 847
Fragments arranged by size:
847 656 69 49 23
BseYI C'CCAG_C
Cuts at: 0 395 924 1644
Size: 395 529 720
Fragments arranged by size:
720 529 395
BsiHKAI G_wGCw'C
Cuts at: 0 1130 1644
Size: 1130 514
BsmI GAATG_Cn'
Cuts at: 0 554 1644
Size: 554 1090
BsmBI CGTCTCn'nnnn_
Cuts at: 0 27 1595 1644
Size: 27 1568 49
Fragments arranged by size:
1568 49 27
Bsp1286I G_dGCh'C
Cuts at: 0 1130 1644
Size: 1130 514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 925 1113 1450 1644
Size: 925 188 337 194
Fragments arranged by size:
925 337 194 188
BsrFI r'CCGG_y
Cuts at: 0 1055 1644
Size: 1055 589
BstEII G'GTnAC_C
Cuts at: 0 753 1644
Size: 753 891
BstZ17I GTA'TAC
Cuts at: 0 1505 1644
Size: 1505 139
Bsu36I CC'TnA_GG
Cuts at: 0 1358 1644
Size: 1358 286
BtgI C'CryG_G
Cuts at: 0 47 521 749 1536 1644
Size: 47 474 228 787 108
Fragments arranged by size:
787 474 228 108 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 56 1365 1644
Size: 56 1309 279
Fragments arranged by size:
1309 279 56
BtsI GCAGTG_nn'
Cuts at: 0 537 545 1644
Size: 537 8 1099
Fragments arranged by size:
1099 537 8
DraI TTT'AAA
Cuts at: 0 78 1644
Size: 78 1566
DraIII CAC_nnn'GTG
Cuts at: 0 761 1644
Size: 761 883
DrdI GACnn_nn'nnGTC
Cuts at: 0 679 1644
Size: 679 965
EaeI y'GGCC_r
Cuts at: 0 588 1644
Size: 588 1056
EarI CTCTTCn'nnn_
Cuts at: 0 220 264 856 869 1644
Size: 220 44 592 13 775
Fragments arranged by size:
775 592 220 44 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 1644
Size: 496 1148
EcoICRI GAG'CTC
Cuts at: 0 1128 1644
Size: 1128 516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 566 659 1644
Size: 451 115 93 985
Fragments arranged by size:
985 451 115 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 638 1644
Size: 638 1006
EcoRV GAT'ATC
Cuts at: 0 1254 1644
Size: 1254 390
FspI TGC'GCA
Cuts at: 0 412 1644
Size: 412 1232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 737 769 1238 1270 1318 1350 1644
Size: 737 32 469 32 48 32 294
Fragments arranged by size:
737 469 294 48 32 32 32
HincII GTy'rAC
Cuts at: 0 1386 1644
Size: 1386 258
HindIII A'AGCT_T
Cuts at: 0 856 1644
Size: 856 788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1040 1644
Size: 835 205 604
Fragments arranged by size:
835 604 205
MscI TGG'CCA
Cuts at: 0 590 1644
Size: 590 1054
MslI CAynn'nnrTG
Cuts at: 0 1009 1644
Size: 1009 635
NcoI C'CATG_G
Cuts at: 0 749 1536 1644
Size: 749 787 108
Fragments arranged by size:
787 749 108
NheI G'CTAG_C
Cuts at: 0 6 1644
Size: 6 1638
NspI r_CATG'y
Cuts at: 0 726 1347 1532 1644
Size: 726 621 185 112
Fragments arranged by size:
726 621 185 112
PasI CC'CwG_GG
Cuts at: 0 930 1644
Size: 930 714
PciI A'CATG_T
Cuts at: 0 722 1528 1644
Size: 722 806 116
Fragments arranged by size:
806 722 116
PmlI CAC'GTG
Cuts at: 0 1011 1644
Size: 1011 633
PsiI TTA'TAA
Cuts at: 0 1466 1644
Size: 1466 178
PstI C_TGCA'G
Cuts at: 0 1110 1644
Size: 1110 534
SacI G_AGCT'C
Cuts at: 0 1130 1644
Size: 1130 514
SalI G'TCGA_C
Cuts at: 0 1384 1644
Size: 1384 260
SbfI CC_TGCA'GG
Cuts at: 0 1110 1644
Size: 1110 534
SexAI A'CCwGG_T
Cuts at: 0 757 1644
Size: 757 887
SfcI C'TryA_G
Cuts at: 0 1106 1644
Size: 1106 538
SmaI CCC'GGG
Cuts at: 0 238 1644
Size: 238 1406
SnaBI TAC'GTA
Cuts at: 0 135 1644
Size: 135 1509
SspI AAT'ATT
Cuts at: 0 1094 1644
Size: 1094 550
StyI C'CwwG_G
Cuts at: 0 62 749 1536 1644
Size: 62 687 787 108
Fragments arranged by size:
787 687 108 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 1644
Size: 44 1600
TatI w'GTAC_w
Cuts at: 0 905 1001 1644
Size: 905 96 643
Fragments arranged by size:
905 643 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 1006 1038 1644
Size: 475 32 499 32 606
Fragments arranged by size:
606 499 475 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1543 1644
Size: 1543 101
XmaI C'CCGG_G
Cuts at: 0 236 1644
Size: 236 1408
XmnI GAAnn'nnTTC
Cuts at: 0 119 1644
Size: 119 1525
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI ApoI AseI
AvaI BanI BanII BcgI BclI BmgBI BmtI BpmI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrFI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoRV FspI Hin4I HincII HindIII
MmeI MscI MslI NcoI NheI NspI PasI PciI
PmlI PsiI PstI SacI SalI SbfI SexAI SfcI
SmaI SnaBI SspI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaBI BsaHI BsgI
BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI ClaI CspCI EagI
EcoO109I EcoRI FalI FseI FspAI HaeII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SmlI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI ZraI