Restriction map for
pF1KB5955
(Linear) (Six-base) MAPSORT of: KIBB5955.seq Check: 720 from: 1 to: 1473
KIBB5955.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AclI AA'CG_TT
Cuts at: 0 1402 1473
Size: 1402 71
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 949 1473
Size: 787 162 524
Fragments arranged by size:
787 524 162
AfeI AGC'GCT
Cuts at: 0 384 1473
Size: 384 1089
AhdI GACnn_n'nnGTC
Cuts at: 0 180 1473
Size: 180 1293
AleI CACnn'nnGTG
Cuts at: 0 1074 1473
Size: 1074 399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 970 1473
Size: 970 503
ApoI r'AATT_y
Cuts at: 0 1259 1473
Size: 1259 214
AvaI C'yCGr_G
Cuts at: 0 665 1473
Size: 665 808
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 726 759 1473
Size: 726 33 714
Fragments arranged by size:
726 714 33
BanI G'GyrC_C
Cuts at: 0 369 1473
Size: 369 1104
BanII G_rGCy'C
Cuts at: 0 878 1352 1473
Size: 878 474 121
Fragments arranged by size:
878 474 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 64 826 1473
Size: 64 762 647
Fragments arranged by size:
762 647 64
BmgBI CAC'GTC
Cuts at: 0 1290 1473
Size: 1290 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 407 1154 1473
Size: 235 172 747 319
Fragments arranged by size:
747 319 235 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1473
Size: 54 1419
BsaI GGTCTCn'nnnn_
Cuts at: 0 1065 1389 1473
Size: 1065 324 84
Fragments arranged by size:
1065 324 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 1473
Size: 122 30 1321
Fragments arranged by size:
1321 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 886 1473
Size: 232 654 587
Fragments arranged by size:
654 587 232
BseYI C'CCAG_C
Cuts at: 0 277 700 1473
Size: 277 423 773
Fragments arranged by size:
773 423 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1473
Size: 612 861
BsiHKAI G_wGCw'C
Cuts at: 0 1352 1473
Size: 1352 121
Bsp1286I G_dGCh'C
Cuts at: 0 878 1352 1473
Size: 878 474 121
Fragments arranged by size:
878 474 121
BspHI T'CATG_A
Cuts at: 0 815 1473
Size: 815 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1473
Size: 1046 427
BsrBI CCG'CTC
Cuts at: 0 1014 1473
Size: 1014 459
BssSI C'ACGA_G
Cuts at: 0 841 1473
Size: 841 632
BstEII G'GTnAC_C
Cuts at: 0 861 1473
Size: 861 612
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 1473
Size: 681 792
BstYI r'GATC_y
Cuts at: 0 114 659 1473
Size: 114 545 814
Fragments arranged by size:
814 545 114
BtgI C'CryG_G
Cuts at: 0 637 1445 1473
Size: 637 808 28
Fragments arranged by size:
808 637 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 950 1473
Size: 950 523
DrdI GACnn_nn'nnGTC
Cuts at: 0 1302 1473
Size: 1302 171
EaeI y'GGCC_r
Cuts at: 0 290 902 1473
Size: 290 612 571
Fragments arranged by size:
612 571 290
EarI CTCTTCn'nnn_
Cuts at: 0 311 925 1385 1473
Size: 311 614 460 88
Fragments arranged by size:
614 460 311 88
EcoICRI GAG'CTC
Cuts at: 0 1350 1473
Size: 1350 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 407 787 949 1154 1473
Size: 235 172 380 162 205 319
Fragments arranged by size:
380 319 235 205 172 162
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1306 1473
Size: 1306 167
EcoO109I rG'GnC_Cy
Cuts at: 0 1035 1473
Size: 1035 438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 973 1473
Size: 973 500
HaeII r_GCGC'y
Cuts at: 0 386 701 1473
Size: 386 315 772
Fragments arranged by size:
772 386 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1201 1233 1473
Size: 166 32 1003 32 240
Fragments arranged by size:
1003 240 166 32 32
HindIII A'AGCT_T
Cuts at: 0 426 1473
Size: 426 1047
MscI TGG'CCA
Cuts at: 0 292 904 1473
Size: 292 612 569
Fragments arranged by size:
612 569 292
MslI CAynn'nnrTG
Cuts at: 0 467 1074 1473
Size: 467 607 399
Fragments arranged by size:
607 467 399
NcoI C'CATG_G
Cuts at: 0 637 1473
Size: 637 836
PasI CC'CwG_GG
Cuts at: 0 1270 1308 1473
Size: 1270 38 165
Fragments arranged by size:
1270 165 38
PfoI T'CCnGG_A
Cuts at: 0 212 797 1424 1473
Size: 212 585 627 49
Fragments arranged by size:
627 585 212 49
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1473
Size: 1035 438
PstI C_TGCA'G
Cuts at: 0 830 1473
Size: 830 643
SacI G_AGCT'C
Cuts at: 0 1352 1473
Size: 1352 121
SapI GCTCTTCn'nnn_
Cuts at: 0 925 1473
Size: 925 548
SfcI C'TryA_G
Cuts at: 0 826 1314 1473
Size: 826 488 159
Fragments arranged by size:
826 488 159
SmaI CCC'GGG
Cuts at: 0 667 1473
Size: 667 806
SmlI C'TyrA_G
Cuts at: 0 33 1473
Size: 33 1440
StyI C'CwwG_G
Cuts at: 0 637 746 905 1473
Size: 637 109 159 568
Fragments arranged by size:
637 568 159 109
TatI w'GTAC_w
Cuts at: 0 896 1124 1473
Size: 896 228 349
Fragments arranged by size:
896 349 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 1311 1343 1473
Size: 122 32 1157 32 130
Fragments arranged by size:
1157 130 122 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 635 1473
Size: 285 350 838
Fragments arranged by size:
838 350 285
XmaI C'CCGG_G
Cuts at: 0 665 1473
Size: 665 808
Enzymes that do cut:
AclI AcuI AfeI AhdI AleI AlfI ApoI AvaI
BaeI BanI BanII BbsI BmgBI BpmI BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BssSI BstEII BstXI BstYI BtgI BtgZI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII MscI MslI NcoI PasI PfoI PpuMI
PstI SacI SapI SfcI SmaI SmlI StyI TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI XmnI
ZraI