Restriction map for
pF1KB5957
(Linear) (Six-base) MAPSORT of: KIBB5957.seq Check: 5693 from: 1 to: 1539
KIBB5957.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 52 1539
Size: 52 1487
AatII G_ACGT'C
Cuts at: 0 480 1539
Size: 480 1059
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1404 1539
Size: 328 1076 135
Fragments arranged by size:
1076 328 135
AflIII A'CryG_T
Cuts at: 0 149 1539
Size: 149 1390
AhdI GACnn_n'nnGTC
Cuts at: 0 866 1539
Size: 866 673
AlwNI CAG_nnn'CTG
Cuts at: 0 1038 1539
Size: 1038 501
ApaI G_GGCC'C
Cuts at: 0 266 486 1539
Size: 266 220 1053
Fragments arranged by size:
1053 266 220
AvaI C'yCGr_G
Cuts at: 0 394 1051 1434 1539
Size: 394 657 383 105
Fragments arranged by size:
657 394 383 105
BanI G'GyrC_C
Cuts at: 0 206 649 1539
Size: 206 443 890
Fragments arranged by size:
890 443 206
BanII G_rGCy'C
Cuts at: 0 77 266 305 486 740 1087 1257 1539
Size: 77 189 39 181 254 347 170 282
Fragments arranged by size:
347 282 254 189 181 170 77 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1172 1539
Size: 1172 367
BbvCI CC'TCA_GC
Cuts at: 0 685 1197 1539
Size: 685 512 342
Fragments arranged by size:
685 512 342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 608 678 712 1539
Size: 574 34 70 34 827
Fragments arranged by size:
827 574 70 34 34
BclI T'GATC_A
Cuts at: 0 1460 1539
Size: 1460 79
BglI GCCn_nnn'nGGC
Cuts at: 0 204 261 1410 1539
Size: 204 57 1149 129
Fragments arranged by size:
1149 204 129 57
BlpI GC'TnA_GC
Cuts at: 0 701 1539
Size: 701 838
Bme1580I G_kGCm'C
Cuts at: 0 136 160 266 486 1539
Size: 136 24 106 220 1053
Fragments arranged by size:
1053 220 136 106 24
BmgBI CAC'GTC
Cuts at: 0 951 1539
Size: 951 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 654 1539
Size: 654 885
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 1539
Size: 526 1013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1282 1539
Size: 794 488 257
Fragments arranged by size:
794 488 257
Bpu10I CC'TnA_GC
Cuts at: 0 685 1197 1539
Size: 685 512 342
Fragments arranged by size:
685 512 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 631 1539
Size: 534 97 908
Fragments arranged by size:
908 534 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 166 1344 1522 1539
Size: 31 135 1178 178 17
Fragments arranged by size:
1178 178 135 31 17
BsaBI GATnn'nnATC
Cuts at: 0 337 1539
Size: 337 1202
BsaHI Gr'CG_yC
Cuts at: 0 477 1539
Size: 477 1062
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 1539
Size: 574 30 935
Fragments arranged by size:
935 574 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 412 628 1445 1539
Size: 337 75 216 817 94
Fragments arranged by size:
817 337 216 94 75
BseYI C'CCAG_C
Cuts at: 0 128 979 1539
Size: 128 851 560
Fragments arranged by size:
851 560 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 1469 1539
Size: 1197 272 70
Fragments arranged by size:
1197 272 70
BsiEI CG_ry'CG
Cuts at: 0 256 357 1539
Size: 256 101 1182
Fragments arranged by size:
1182 256 101
BsiHKAI G_wGCw'C
Cuts at: 0 371 740 1539
Size: 371 369 799
Fragments arranged by size:
799 371 369
BsmBI CGTCTCn'nnnn_
Cuts at: 0 468 1539
Size: 468 1071
Bsp1286I G_dGCh'C
Cuts at: 0 77 136 160 266 305 371 486 740
Size: 77 59 24 106 39 66 115 254
Cuts at: 740 1087 1257 1539
Size: 347 170 282
Fragments arranged by size:
347 282 254 170 115 106 77 66
59 39 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 1046 1539
Size: 52 994 493
Fragments arranged by size:
994 493 52
BsrBI CCG'CTC
Cuts at: 0 252 359 393 1296 1539
Size: 252 107 34 903 243
Fragments arranged by size:
903 252 243 107 34
BsrFI r'CCGG_y
Cuts at: 0 203 1539
Size: 203 1336
BssSI C'ACGA_G
Cuts at: 0 500 1539
Size: 500 1039
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 1539
Size: 345 1194
BtgI C'CryG_G
Cuts at: 0 372 1523 1539
Size: 372 1151 16
Fragments arranged by size:
1151 372 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1083 1539
Size: 1083 456
BtsI GCAGTG_nn'
Cuts at: 0 169 1229 1539
Size: 169 1060 310
Fragments arranged by size:
1060 310 169
DraI TTT'AAA
Cuts at: 0 449 467 1539
Size: 449 18 1072
Fragments arranged by size:
1072 449 18
DraIII CAC_nnn'GTG
Cuts at: 0 384 1539
Size: 384 1155
DrdI GACnn_nn'nnGTC
Cuts at: 0 150 1539
Size: 150 1389
EaeI y'GGCC_r
Cuts at: 0 17 93 212 253 375 626 1497 1539
Size: 17 76 119 41 122 251 871 42
Fragments arranged by size:
871 251 122 119 76 42 41 17
EagI C'GGCC_G
Cuts at: 0 253 1539
Size: 253 1286
EarI CTCTTCn'nnn_
Cuts at: 0 466 704 1539
Size: 466 238 835
Fragments arranged by size:
835 466 238
EcoICRI GAG'CTC
Cuts at: 0 738 1539
Size: 738 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 794 1282 1404 1539
Size: 328 466 488 122 135
Fragments arranged by size:
488 466 328 135 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1030 1539
Size: 31 999 509
Fragments arranged by size:
999 509 31
EcoO109I rG'GnC_Cy
Cuts at: 0 262 1035 1539
Size: 262 773 504
Fragments arranged by size:
773 504 262
FspI TGC'GCA
Cuts at: 0 244 1539
Size: 244 1295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 792 824 852 884 1047 1079
Size: 241 32 519 32 28 32 163 32
Cuts at: 1079 1539
Size: 460
Fragments arranged by size:
519 460 241 163 32 32 32 32
28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 1539
Size: 1053 486
MscI TGG'CCA
Cuts at: 0 214 628 1499 1539
Size: 214 414 871 40
Fragments arranged by size:
871 414 214 40
MslI CAynn'nnrTG
Cuts at: 0 343 1539
Size: 343 1196
MspA1I CmG'CkG
Cuts at: 0 125 240 374 564 1302 1539
Size: 125 115 134 190 738 237
Fragments arranged by size:
738 237 190 134 125 115
NcoI C'CATG_G
Cuts at: 0 1523 1539
Size: 1523 16
NspI r_CATG'y
Cuts at: 0 195 1539
Size: 195 1344
PasI CC'CwG_GG
Cuts at: 0 486 1539
Size: 486 1053
PflMI CCAn_nnn'nTGG
Cuts at: 0 1305 1539
Size: 1305 234
PfoI T'CCnGG_A
Cuts at: 0 7 1539
Size: 7 1532
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1539
Size: 1035 504
PspOMI G'GGCC_C
Cuts at: 0 262 482 1539
Size: 262 220 1057
Fragments arranged by size:
1057 262 220
PspXI vC'TCGA_Gb
Cuts at: 0 394 1539
Size: 394 1145
PstI C_TGCA'G
Cuts at: 0 32 62 746 1539
Size: 32 30 684 793
Fragments arranged by size:
793 684 32 30
PvuII CAG'CTG
Cuts at: 0 240 564 1539
Size: 240 324 975
Fragments arranged by size:
975 324 240
SacI G_AGCT'C
Cuts at: 0 740 1539
Size: 740 799
SacII CC_GC'GG
Cuts at: 0 375 1539
Size: 375 1164
SbfI CC_TGCA'GG
Cuts at: 0 746 1539
Size: 746 793
ScaI AGT'ACT
Cuts at: 0 64 1539
Size: 64 1475
SfcI C'TryA_G
Cuts at: 0 28 58 742 1539
Size: 28 30 684 797
Fragments arranged by size:
797 684 30 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 261 1410 1539
Size: 261 1149 129
Fragments arranged by size:
1149 261 129
SmlI C'TyrA_G
Cuts at: 0 394 513 610 1539
Size: 394 119 97 929
Fragments arranged by size:
929 394 119 97
SspI AAT'ATT
Cuts at: 0 1249 1539
Size: 1249 290
StyI C'CwwG_G
Cuts at: 0 796 1523 1539
Size: 796 727 16
Fragments arranged by size:
796 727 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 673 1539
Size: 673 866
TatI w'GTAC_w
Cuts at: 0 62 314 1539
Size: 62 252 1225
Fragments arranged by size:
1225 252 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1140 1172 1539
Size: 1140 32 367
Fragments arranged by size:
1140 367 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1539
Size: 207 1332
XhoI C'TCGA_G
Cuts at: 0 394 1539
Size: 394 1145
XmnI GAAnn'nnTTC
Cuts at: 0 1213 1539
Size: 1213 326
ZraI GAC'GTC
Cuts at: 0 478 1539
Size: 478 1061
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SbfI ScaI
SfcI SfiI SmlI SspI StyI TaqII TatI TstI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BglII BmtI BsaAI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI EciI
EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI
SanDI SapI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XmaI