Restriction map for
pF1KB5959
(Linear) (Six-base) MAPSORT of: KIBB5959.seq Check: 6340 from: 1 to: 1539
KIBB5959.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 1380 1539
Size: 204 1176 159
Fragments arranged by size:
1176 204 159
AatII G_ACGT'C
Cuts at: 0 526 1539
Size: 526 1013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1003 1332 1539
Size: 193 810 329 207
Fragments arranged by size:
810 329 207 193
AfeI AGC'GCT
Cuts at: 0 1528 1539
Size: 1528 11
AflII C'TTAA_G
Cuts at: 0 184 1539
Size: 184 1355
AflIII A'CryG_T
Cuts at: 0 897 1539
Size: 897 642
AleI CACnn'nnGTG
Cuts at: 0 662 973 1539
Size: 662 311 566
Fragments arranged by size:
662 566 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 886 1539
Size: 886 653
AlwNI CAG_nnn'CTG
Cuts at: 0 336 429 1036 1192 1236 1372 1539
Size: 336 93 607 156 44 136 167
Fragments arranged by size:
607 336 167 156 136 93 44
ApaI G_GGCC'C
Cuts at: 0 83 388 1416 1539
Size: 83 305 1028 123
Fragments arranged by size:
1028 305 123 83
ApaLI G'TGCA_C
Cuts at: 0 267 1494 1539
Size: 267 1227 45
Fragments arranged by size:
1227 267 45
AvaI C'yCGr_G
Cuts at: 0 50 201 559 1409 1436 1539
Size: 50 151 358 850 27 103
Fragments arranged by size:
850 358 151 103 50 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 243 276 1539
Size: 243 33 1263
Fragments arranged by size:
1263 243 33
BanI G'GyrC_C
Cuts at: 0 399 1024 1423 1539
Size: 399 625 399 116
Fragments arranged by size:
625 399 399 116
BanII G_rGCy'C
Cuts at: 0 51 83 388 416 423 1176 1358 1416
Size: 51 32 305 28 7 753 182 58
Cuts at: 1416 1539
Size: 123
Fragments arranged by size:
753 305 182 123 58 51 32 28
7
BclI T'GATC_A
Cuts at: 0 753 936 1539
Size: 753 183 603
Fragments arranged by size:
753 603 183
BfrBI ATG'CAT
Cuts at: 0 1225 1539
Size: 1225 314
BlpI GC'TnA_GC
Cuts at: 0 1342 1539
Size: 1342 197
Bme1580I G_kGCm'C
Cuts at: 0 83 271 388 1416 1498 1539
Size: 83 188 117 1028 82 41
Fragments arranged by size:
1028 188 117 83 82 41
BmgBI CAC'GTC
Cuts at: 0 900 1539
Size: 900 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 1024 1116 1249 1539
Size: 59 965 92 133 290
Fragments arranged by size:
965 290 133 92 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1539
Size: 99 1440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 430 432 681 1539
Size: 346 84 2 249 858
Fragments arranged by size:
858 346 249 84 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 760 1539
Size: 760 779
BsaHI Gr'CG_yC
Cuts at: 0 21 523 1539
Size: 21 502 1016
Fragments arranged by size:
1016 502 21
BsaWI w'CCGG_w
Cuts at: 0 1323 1328 1539
Size: 1323 5 211
Fragments arranged by size:
1323 211 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 349 379 660 690 1539
Size: 38 30 281 30 281 30 849
Fragments arranged by size:
849 281 281 38 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 967 1539
Size: 373 594 572
Fragments arranged by size:
594 572 373
BseYI C'CCAG_C
Cuts at: 0 128 193 1069 1286 1358 1539
Size: 128 65 876 217 72 181
Fragments arranged by size:
876 217 181 128 72 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1096 1539
Size: 997 99 443
Fragments arranged by size:
997 443 99
BsiEI CG_ry'CG
Cuts at: 0 1339 1539
Size: 1339 200
BsiHKAI G_wGCw'C
Cuts at: 0 271 1481 1498 1539
Size: 271 1210 17 41
Fragments arranged by size:
1210 271 41 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 450 1539
Size: 450 1089
Bsp1286I G_dGCh'C
Cuts at: 0 51 83 271 388 416 423 1176 1358
Size: 51 32 188 117 28 7 753 182
Cuts at: 1358 1416 1481 1498 1539
Size: 58 65 17 41
Fragments arranged by size:
753 188 182 117 65 58 51 41
32 28 17 7
BspEI T'CCGG_A
Cuts at: 0 1328 1539
Size: 1328 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 330 1380 1539
Size: 204 126 1050 159
Fragments arranged by size:
1050 204 159 126
BsrBI CCG'CTC
Cuts at: 0 124 1539
Size: 124 1415
BsrFI r'CCGG_y
Cuts at: 0 1334 1505 1539
Size: 1334 171 34
Fragments arranged by size:
1334 171 34
BsrGI T'GTAC_A
Cuts at: 0 546 836 1539
Size: 546 290 703
Fragments arranged by size:
703 546 290
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 1222 1502 1539
Size: 336 886 280 37
Fragments arranged by size:
886 336 280 37
BstEII G'GTnAC_C
Cuts at: 0 309 1539
Size: 309 1230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 1539
Size: 723 816
BstYI r'GATC_y
Cuts at: 0 1250 1539
Size: 1250 289
Bsu36I CC'TnA_GG
Cuts at: 0 1167 1539
Size: 1167 372
BtgI C'CryG_G
Cuts at: 0 512 604 1539
Size: 512 92 935
Fragments arranged by size:
935 512 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1390 1539
Size: 1390 149
BtsI GCAGTG_nn'
Cuts at: 0 106 491 678 1539
Size: 106 385 187 861
Fragments arranged by size:
861 385 187 106
ClaI AT'CG_AT
Cuts at: 0 458 1539
Size: 458 1081
DrdI GACnn_nn'nnGTC
Cuts at: 0 528 1539
Size: 528 1011
EaeI y'GGCC_r
Cuts at: 0 509 850 1539
Size: 509 341 689
Fragments arranged by size:
689 509 341
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1539
Size: 1103 436
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1293 1539
Size: 1293 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 346 430 432 681 1003 1332 1539
Size: 193 153 84 2 249 322 329 207
Fragments arranged by size:
329 322 249 207 193 153 84 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 1539
Size: 211 1328
EcoO109I rG'GnC_Cy
Cuts at: 0 61 79 80 384 385 426 1196 1539
Size: 61 18 1 304 1 41 770 343
Fragments arranged by size:
770 343 304 61 41 18 1 1
HaeII r_GCGC'y
Cuts at: 0 1530 1539
Size: 1530 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 1368 1400 1539
Size: 288 32 1048 32 139
Fragments arranged by size:
1048 288 139 32 32
HindIII A'AGCT_T
Cuts at: 0 1344 1539
Size: 1344 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1539
Size: 458 1081
MscI TGG'CCA
Cuts at: 0 852 1539
Size: 852 687
MslI CAynn'nnrTG
Cuts at: 0 662 973 1539
Size: 662 311 566
Fragments arranged by size:
662 566 311
MspA1I CmG'CkG
Cuts at: 0 293 635 653 981 1286 1539
Size: 293 342 18 328 305 253
Fragments arranged by size:
342 328 305 293 253 18
NcoI C'CATG_G
Cuts at: 0 512 604 1539
Size: 512 92 935
Fragments arranged by size:
935 512 92
NsiI A_TGCA'T
Cuts at: 0 1227 1539
Size: 1227 312
PasI CC'CwG_GG
Cuts at: 0 1036 1539
Size: 1036 503
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 859 1036 1539
Size: 429 430 177 503
Fragments arranged by size:
503 430 429 177
PfoI T'CCnGG_A
Cuts at: 0 448 1539
Size: 448 1091
PpuMI rG'GwC_Cy
Cuts at: 0 61 1196 1539
Size: 61 1135 343
Fragments arranged by size:
1135 343 61
PspOMI G'GGCC_C
Cuts at: 0 79 384 1412 1539
Size: 79 305 1028 127
Fragments arranged by size:
1028 305 127 79
PstI C_TGCA'G
Cuts at: 0 341 352 383 538 1539
Size: 341 11 31 155 1001
Fragments arranged by size:
1001 341 155 31 11
PvuII CAG'CTG
Cuts at: 0 293 981 1286 1539
Size: 293 688 305 253
Fragments arranged by size:
688 305 293 253
RsrII CG'GwC_CG
Cuts at: 0 1326 1539
Size: 1326 213
SanDI GG'GwC_CC
Cuts at: 0 61 1539
Size: 61 1478
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 1539
Size: 1103 436
SfcI C'TryA_G
Cuts at: 0 337 348 379 435 534 1539
Size: 337 11 31 56 99 1005
Fragments arranged by size:
1005 337 99 56 31 11
SmaI CCC'GGG
Cuts at: 0 52 561 1411 1539
Size: 52 509 850 128
Fragments arranged by size:
850 509 128 52
SmlI C'TyrA_G
Cuts at: 0 184 1539
Size: 184 1355
StuI AGG'CCT
Cuts at: 0 1519 1539
Size: 1519 20
StyI C'CwwG_G
Cuts at: 0 24 173 512 604 1539
Size: 24 149 339 92 935
Fragments arranged by size:
935 339 149 92 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 784 1539
Size: 580 204 755
Fragments arranged by size:
755 580 204
TatI w'GTAC_w
Cuts at: 0 546 836 1539
Size: 546 290 703
Fragments arranged by size:
703 546 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 349 381 600 632 660 692
Size: 38 32 279 32 219 32 28 32
Cuts at: 692 1477 1509 1539
Size: 785 32 30
Fragments arranged by size:
785 279 219 38 32 32 32 32
32 30 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 754 1539
Size: 754 785
XmaI C'CCGG_G
Cuts at: 0 50 559 1409 1539
Size: 50 509 850 130
Fragments arranged by size:
850 509 130 50
ZraI GAC'GTC
Cuts at: 0 524 1539
Size: 524 1015
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI AvaI BaeI BanI BanII BclI
BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NcoI NsiI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII
SanDI SapI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AhdI AloI ApoI AscI
AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BmtI Bpu10I BpuEI BsaAI BsaBI
BsiWI BsmI BspHI BsrDI BssHII BssSI BstBI BstZ17I
CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI