(Linear) (Six-base) MAPSORT of: KIBB5961.seq  Check: 1313  from: 1  to: 1545



KIBB5961.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1052    1545 
   Size:       1052     493
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      37    1121    1545 
   Size:         37    1084     424

  Fragments arranged by size: 

                1084     424      37
 
BanI G'GyrC_C

Cuts at:      0      64    1545 
   Size:         64    1481
 
BanII G_rGCy'C

Cuts at:      0      82    1545 
   Size:         82    1463
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     553    1545 
   Size:         55     498     992

  Fragments arranged by size: 

                 992     498      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527    1545 
   Size:        527    1018
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     469    1545 
   Size:        122     347    1076

  Fragments arranged by size: 

                1076     347     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1130    1545 
   Size:       1130     415
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     229     232    1545 
   Size:        229       3    1313

  Fragments arranged by size: 

                1313     229       3
 
BseYI C'CCAG_C

Cuts at:      0      50    1545 
   Size:         50    1495
 
BsiHKAI G_wGCw'C

Cuts at:      0     342    1545 
   Size:        342    1203
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      98     193    1545 
   Size:         98      95    1352

  Fragments arranged by size: 

                1352      98      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     342    1545 
   Size:         82     260    1203

  Fragments arranged by size: 

                1203     260      82
 
BspHI T'CATG_A

Cuts at:      0     767    1545 
   Size:        767     778
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     477    1545 
   Size:        477    1068
 
BstYI r'GATC_y

Cuts at:      0     877    1324    1545 
   Size:        877     447     221

  Fragments arranged by size: 

                 877     447     221
 
BtgI C'CryG_G

Cuts at:      0     120    1545 
   Size:        120    1425
 
BtsI GCAGTG_nn'

Cuts at:      0     481     601     827    1105    1545 
   Size:        481     120     226     278     440

  Fragments arranged by size: 

                 481     440     278     226     120
 
ClaI AT'CG_AT

Cuts at:      0    1278    1545 
   Size:       1278     267
 
DraI TTT'AAA

Cuts at:      0     747     884    1545 
   Size:        747     137     661

  Fragments arranged by size: 

                 747     661     137
 
EarI CTCTTCn'nnn_

Cuts at:      0    1086    1545 
   Size:       1086     459
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     469    1052    1545 
   Size:        122     347     583     493

  Fragments arranged by size: 

                 583     493     347     122
 
EcoO109I rG'GnC_Cy

Cuts at:      0     170     662    1141    1545 
   Size:        170     492     479     404

  Fragments arranged by size: 

                 492     479     404     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     324     356     503     535    1545 
   Size:        324      32     147      32    1010

  Fragments arranged by size: 

                1010     324     147      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1198    1545 
   Size:       1198     347
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1210    1545 
   Size:        557     653     335

  Fragments arranged by size: 

                 653     557     335
 
MslI CAynn'nnrTG

Cuts at:      0     731    1545 
   Size:        731     814
 
MspA1I CmG'CkG

Cuts at:      0     167     237    1545 
   Size:        167      70    1308

  Fragments arranged by size: 

                1308     167      70
 
PpuMI rG'GwC_Cy

Cuts at:      0     170     662    1545 
   Size:        170     492     883

  Fragments arranged by size: 

                 883     492     170
 
PstI C_TGCA'G

Cuts at:      0     488     575    1545 
   Size:        488      87     970

  Fragments arranged by size: 

                 970     488      87
 
SfcI C'TryA_G

Cuts at:      0     484     571    1058    1545 
   Size:        484      87     487     487

  Fragments arranged by size: 

                 487     487     484      87
 
SmlI C'TyrA_G

Cuts at:      0    1145    1545 
   Size:       1145     400
 
SspI AAT'ATT

Cuts at:      0     438    1545 
   Size:        438    1107
 
TatI w'GTAC_w

Cuts at:      0     274    1545 
   Size:        274    1271
 
XmnI GAAnn'nnTTC

Cuts at:      0     704    1545 
   Size:        704     841
 

 Enzymes that do cut:

     AcuI     AlfI     BanI    BanII     BglI     BmrI     BpmI    BpuEI
    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BstYI
     BtgI     BtsI     ClaI     DraI     EarI  Eco57MI EcoO109I    Hin4I
  HindIII     MmeI     MslI   MspA1I    PpuMI     PstI     SfcI     SmlI
     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI