Restriction map for
pF1KB5965
(Linear) (Six-base) MAPSORT of: KIBB5965.seq Check: 6727 from: 1 to: 1728
KIBB5965.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1309 1728
Size: 1309 419
AccI GT'mk_AC
Cuts at: 0 283 828 1267 1728
Size: 283 545 439 461
Fragments arranged by size:
545 461 439 283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1363 1728
Size: 880 483 365
Fragments arranged by size:
880 483 365
AhdI GACnn_n'nnGTC
Cuts at: 0 207 1728
Size: 207 1521
AleI CACnn'nnGTG
Cuts at: 0 1103 1623 1728
Size: 1103 520 105
Fragments arranged by size:
1103 520 105
AlwNI CAG_nnn'CTG
Cuts at: 0 166 1728
Size: 166 1562
ApaLI G'TGCA_C
Cuts at: 0 1124 1258 1728
Size: 1124 134 470
Fragments arranged by size:
1124 470 134
AvaI C'yCGr_G
Cuts at: 0 572 613 1017 1728
Size: 572 41 404 711
Fragments arranged by size:
711 572 404 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 993 1026 1728
Size: 993 33 702
Fragments arranged by size:
993 702 33
BamHI G'GATC_C
Cuts at: 0 911 1728
Size: 911 817
BanI G'GyrC_C
Cuts at: 0 686 1728
Size: 686 1042
BciVI GTATCCnnnnn_n'
Cuts at: 0 1021 1728
Size: 1021 707
BglI GCCn_nnn'nGGC
Cuts at: 0 1492 1728
Size: 1492 236
BlpI GC'TnA_GC
Cuts at: 0 842 1728
Size: 842 886
Bme1580I G_kGCm'C
Cuts at: 0 775 958 1128 1262 1728
Size: 775 183 170 134 466
Fragments arranged by size:
775 466 183 170 134
BmgBI CAC'GTC
Cuts at: 0 371 1728
Size: 371 1357
BmrI ACTGGGnnnn_n'
Cuts at: 0 1617 1728
Size: 1617 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 1063 1728
Size: 323 740 665
Fragments arranged by size:
740 665 323
Bpu10I CC'TnA_GC
Cuts at: 0 563 1451 1457 1728
Size: 563 888 6 271
Fragments arranged by size:
888 563 271 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1424 1448 1728
Size: 1418 6 24 280
Fragments arranged by size:
1418 280 24 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 928 1728
Size: 748 180 800
Fragments arranged by size:
800 748 180
BsaBI GATnn'nnATC
Cuts at: 0 659 730 1728
Size: 659 71 998
Fragments arranged by size:
998 659 71
BseYI C'CCAG_C
Cuts at: 0 815 998 1728
Size: 815 183 730
Fragments arranged by size:
815 730 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1287 1411 1728
Size: 1287 124 317
Fragments arranged by size:
1287 317 124
BsiHKAI G_wGCw'C
Cuts at: 0 700 1128 1262 1715 1728
Size: 700 428 134 453 13
Fragments arranged by size:
700 453 428 134 13
Bsp1286I G_dGCh'C
Cuts at: 0 700 775 958 1128 1262 1715 1728
Size: 700 75 183 170 134 453 13
Fragments arranged by size:
700 453 183 170 134 75 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1309 1728
Size: 465 844 419
Fragments arranged by size:
844 465 419
BsrDI GCAATG_nn'
Cuts at: 0 777 1728
Size: 777 951
BsrGI T'GTAC_A
Cuts at: 0 1295 1728
Size: 1295 433
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1310 1389 1728
Size: 1310 79 339
Fragments arranged by size:
1310 339 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1567 1707 1728
Size: 1567 140 21
Fragments arranged by size:
1567 140 21
BstYI r'GATC_y
Cuts at: 0 731 911 1728
Size: 731 180 817
Fragments arranged by size:
817 731 180
BstZ17I GTA'TAC
Cuts at: 0 829 1728
Size: 829 899
Bsu36I CC'TnA_GG
Cuts at: 0 186 378 644 1728
Size: 186 192 266 1084
Fragments arranged by size:
1084 266 192 186
BtsI GCAGTG_nn'
Cuts at: 0 558 960 1421 1728
Size: 558 402 461 307
Fragments arranged by size:
558 461 402 307
DraIII CAC_nnn'GTG
Cuts at: 0 1278 1728
Size: 1278 450
DrdI GACnn_nn'nnGTC
Cuts at: 0 631 1728
Size: 631 1097
EaeI y'GGCC_r
Cuts at: 0 1718 1728
Size: 1718 10
EarI CTCTTCn'nnn_
Cuts at: 0 324 455 1728
Size: 324 131 1273
Fragments arranged by size:
1273 324 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 545 868 1051 1672 1728
Size: 545 323 183 621 56
Fragments arranged by size:
621 545 323 183 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 880 1063 1363 1728
Size: 323 557 183 300 365
Fragments arranged by size:
557 365 323 300 183
EcoNI CCTnn'n_nnAGG
Cuts at: 0 517 1728
Size: 517 1211
FspI TGC'GCA
Cuts at: 0 1357 1728
Size: 1357 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 84 116 193 199 225 231 1231 1263
Size: 84 32 77 6 26 6 1000 32
Cuts at: 1263 1728
Size: 465
Fragments arranged by size:
1000 465 84 77 32 32 26 6
6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1728
Size: 701 1027
MscI TGG'CCA
Cuts at: 0 1720 1728
Size: 1720 8
MslI CAynn'nnrTG
Cuts at: 0 196 1103 1119 1623 1705 1728
Size: 196 907 16 504 82 23
Fragments arranged by size:
907 504 196 82 23 16
MspA1I CmG'CkG
Cuts at: 0 105 225 1408 1728
Size: 105 120 1183 320
Fragments arranged by size:
1183 320 120 105
NdeI CA'TA_TG
Cuts at: 0 1352 1728
Size: 1352 376
NspI r_CATG'y
Cuts at: 0 1288 1728
Size: 1288 440
PasI CC'CwG_GG
Cuts at: 0 1581 1728
Size: 1581 147
PstI C_TGCA'G
Cuts at: 0 476 1172 1315 1728
Size: 476 696 143 413
Fragments arranged by size:
696 476 413 143
PvuII CAG'CTG
Cuts at: 0 105 225 1728
Size: 105 120 1503
Fragments arranged by size:
1503 120 105
SapI GCTCTTCn'nnn_
Cuts at: 0 455 1728
Size: 455 1273
SbfI CC_TGCA'GG
Cuts at: 0 476 1728
Size: 476 1252
SfcI C'TryA_G
Cuts at: 0 284 472 681 849 1168 1311 1728
Size: 284 188 209 168 319 143 417
Fragments arranged by size:
417 319 284 209 188 168 143
SmaI CCC'GGG
Cuts at: 0 574 1728
Size: 574 1154
SmlI C'TyrA_G
Cuts at: 0 1433 1439 1463 1728
Size: 1433 6 24 265
Fragments arranged by size:
1433 265 24 6
SphI G_CATG'C
Cuts at: 0 1288 1728
Size: 1288 440
StuI AGG'CCT
Cuts at: 0 1057 1486 1728
Size: 1057 429 242
Fragments arranged by size:
1057 429 242
StyI C'CwwG_G
Cuts at: 0 1289 1728
Size: 1289 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1145 1728
Size: 1145 583
TatI w'GTAC_w
Cuts at: 0 1295 1728
Size: 1295 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1541 1573 1728
Size: 1541 32 155
Fragments arranged by size:
1541 155 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1588 1728
Size: 1588 140
XmaI C'CCGG_G
Cuts at: 0 572 1728
Size: 572 1156
XmnI GAAnn'nnTTC
Cuts at: 0 874 1057 1147 1728
Size: 874 183 90 581
Fragments arranged by size:
874 581 183 90
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlwNI ApaLI AvaI
BaeI BamHI BanI BciVI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI
BstZ17I Bsu36I BtsI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI FspI Hin4I MmeI MscI MslI MspA1I
NdeI NspI PasI PstI PvuII SapI SbfI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApaI ApoI AscI AseI AsiSI AvrII BanII
BbeI BbsI BbvCI BcgI BclI BfrBI BglII BmtI
BplI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstBI BstEII BtgI BtgZI ClaI CspCI DraI EagI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI