Restriction map for
pF1KB5968
(Linear) (Six-base) MAPSORT of: KIBB5968.seq Check: 7624 from: 1 to: 1668
KIBB5968.orf
With 182 enzymes: SgfI *
August 9, 2016 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 109 123 1668
Size: 109 14 1545
Fragments arranged by size:
1545 109 14
AatII G_ACGT'C
Cuts at: 0 1268 1668
Size: 1268 400
AccI GT'mk_AC
Cuts at: 0 1522 1668
Size: 1522 146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1367 1619 1668
Size: 170 1197 252 49
Fragments arranged by size:
1197 252 170 49
AflII C'TTAA_G
Cuts at: 0 1527 1668
Size: 1527 141
AflIII A'CryG_T
Cuts at: 0 364 1461 1668
Size: 364 1097 207
Fragments arranged by size:
1097 364 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 425 1082 1668
Size: 425 657 586
Fragments arranged by size:
657 586 425
ApaI G_GGCC'C
Cuts at: 0 15 1668
Size: 15 1653
ApoI r'AATT_y
Cuts at: 0 529 1312 1442 1639 1668
Size: 529 783 130 197 29
Fragments arranged by size:
783 529 197 130 29
AvaI C'yCGr_G
Cuts at: 0 515 1668
Size: 515 1153
BanII G_rGCy'C
Cuts at: 0 15 983 1668
Size: 15 968 685
Fragments arranged by size:
968 685 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 176 1260 1295 1668
Size: 67 109 1084 35 373
Fragments arranged by size:
1084 373 109 67 35
BciVI GTATCCnnnnn_n'
Cuts at: 0 187 277 1668
Size: 187 90 1391
Fragments arranged by size:
1391 187 90
BclI T'GATC_A
Cuts at: 0 1149 1668
Size: 1149 519
BglII A'GATC_T
Cuts at: 0 320 1668
Size: 320 1348
BlpI GC'TnA_GC
Cuts at: 0 635 1668
Size: 635 1033
Bme1580I G_kGCm'C
Cuts at: 0 15 719 1668
Size: 15 704 949
Fragments arranged by size:
949 704 15
BmgBI CAC'GTC
Cuts at: 0 1464 1668
Size: 1464 204
BmtI G_CTAG'C
Cuts at: 0 1453 1668
Size: 1453 215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 997 1668
Size: 997 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 647 967 1668
Size: 246 401 320 701
Fragments arranged by size:
701 401 320 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1668
Size: 990 678
BsaHI Gr'CG_yC
Cuts at: 0 212 646 1265 1668
Size: 212 434 619 403
Fragments arranged by size:
619 434 403 212
BsaWI w'CCGG_w
Cuts at: 0 587 1668
Size: 587 1081
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1668
Size: 1039 30 599
Fragments arranged by size:
1039 599 30
BsiHKAI G_wGCw'C
Cuts at: 0 983 1668
Size: 983 685
BsmI GAATG_Cn'
Cuts at: 0 297 1668
Size: 297 1371
Bsp1286I G_dGCh'C
Cuts at: 0 15 719 983 1668
Size: 15 704 264 685
Fragments arranged by size:
704 685 264 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 123 967 1162 1668
Size: 109 14 844 195 506
Fragments arranged by size:
844 506 195 109 14
BsrDI GCAATG_nn'
Cuts at: 0 414 1658 1668
Size: 414 1244 10
Fragments arranged by size:
1244 414 10
BstBI TT'CG_AA
Cuts at: 0 78 1668
Size: 78 1590
BstYI r'GATC_y
Cuts at: 0 320 1668
Size: 320 1348
Bsu36I CC'TnA_GG
Cuts at: 0 725 1278 1668
Size: 725 553 390
Fragments arranged by size:
725 553 390
BtgI C'CryG_G
Cuts at: 0 52 905 1668
Size: 52 853 763
Fragments arranged by size:
853 763 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 306 341 1668
Size: 306 35 1327
Fragments arranged by size:
1327 306 35
DraI TTT'AAA
Cuts at: 0 1572 1668
Size: 1572 96
DrdI GACnn_nn'nnGTC
Cuts at: 0 748 1668
Size: 748 920
EaeI y'GGCC_r
Cuts at: 0 534 665 1668
Size: 534 131 1003
Fragments arranged by size:
1003 534 131
EarI CTCTTCn'nnn_
Cuts at: 0 971 1460 1668
Size: 971 489 208
Fragments arranged by size:
971 489 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 735 1668
Size: 735 933
EcoICRI GAG'CTC
Cuts at: 0 981 1668
Size: 981 687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 246 647 967 1367 1619 1668
Size: 170 76 401 320 400 252 49
Fragments arranged by size:
401 400 320 252 170 76 49
EcoO109I rG'GnC_Cy
Cuts at: 0 11 12 1668
Size: 11 1 1656
Fragments arranged by size:
1656 11 1
EcoRV GAT'ATC
Cuts at: 0 804 1668
Size: 804 864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 1668
Size: 322 32 1314
Fragments arranged by size:
1314 322 32
MfeI C'AATT_G
Cuts at: 0 1060 1668
Size: 1060 608
MslI CAynn'nnrTG
Cuts at: 0 353 1668
Size: 353 1315
MspA1I CmG'CkG
Cuts at: 0 470 642 1044 1668
Size: 470 172 402 624
Fragments arranged by size:
624 470 402 172
NcoI C'CATG_G
Cuts at: 0 905 1668
Size: 905 763
NheI G'CTAG_C
Cuts at: 0 1449 1668
Size: 1449 219
NspI r_CATG'y
Cuts at: 0 368 1668
Size: 368 1300
PciI A'CATG_T
Cuts at: 0 364 1668
Size: 364 1304
PfoI T'CCnGG_A
Cuts at: 0 223 1668
Size: 223 1445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1314 1346 1668
Size: 1314 32 322
Fragments arranged by size:
1314 322 32
PspOMI G'GGCC_C
Cuts at: 0 11 1668
Size: 11 1657
PstI C_TGCA'G
Cuts at: 0 120 1668
Size: 120 1548
SacI G_AGCT'C
Cuts at: 0 983 1668
Size: 983 685
SapI GCTCTTCn'nnn_
Cuts at: 0 971 1460 1668
Size: 971 489 208
Fragments arranged by size:
971 489 208
SbfI CC_TGCA'GG
Cuts at: 0 120 1668
Size: 120 1548
SfcI C'TryA_G
Cuts at: 0 116 1468 1668
Size: 116 1352 200
Fragments arranged by size:
1352 200 116
SmlI C'TyrA_G
Cuts at: 0 515 969 1527 1668
Size: 515 454 558 141
Fragments arranged by size:
558 515 454 141
StyI C'CwwG_G
Cuts at: 0 905 1668
Size: 905 763
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 1668
Size: 123 1545
TatI w'GTAC_w
Cuts at: 0 273 576 1235 1377 1668
Size: 273 303 659 142 291
Fragments arranged by size:
659 303 291 273 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 1668
Size: 1039 32 597
Fragments arranged by size:
1039 597 32
XbaI T'CTAG_A
Cuts at: 0 1179 1668
Size: 1179 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 1668
Size: 622 1046
XhoI C'TCGA_G
Cuts at: 0 515 1668
Size: 515 1153
XmnI GAAnn'nnTTC
Cuts at: 0 308 1668
Size: 308 1360
ZraI GAC'GTC
Cuts at: 0 1266 1668
Size: 1266 402
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlfI ApaI
ApoI AvaI BanII BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaHI BsaWI
BsaXI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstBI BstYI
Bsu36I BtgI CspCI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRV Hin4I MfeI MslI MspA1I
NcoI NheI NspI PciI PfoI PpiI PspOMI PstI
SacI SapI SbfI SfcI SmlI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BfrBI BglI BmrI Bpu10I BsaI
BsaAI BsaBI BseRI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgZI BtsI ClaI DraIII EagI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XmaI