Restriction map for
pF1KB5973
(Linear) (Six-base) MAPSORT of: KIBB5973.seq Check: 1927 from: 1 to: 1557
KIBB5973.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 140 152 1557
Size: 140 12 1405
Fragments arranged by size:
1405 140 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1557
Size: 844 713
AlwNI CAG_nnn'CTG
Cuts at: 0 355 1310 1557
Size: 355 955 247
Fragments arranged by size:
955 355 247
ApaI G_GGCC'C
Cuts at: 0 116 969 1557
Size: 116 853 588
Fragments arranged by size:
853 588 116
AvaI C'yCGr_G
Cuts at: 0 1399 1557
Size: 1399 158
BanI G'GyrC_C
Cuts at: 0 132 345 593 1264 1277 1384 1501 1557
Size: 132 213 248 671 13 107 117 56
Fragments arranged by size:
671 248 213 132 117 107 56 13
BanII G_rGCy'C
Cuts at: 0 116 191 713 724 740 969 1557
Size: 116 75 522 11 16 229 588
Fragments arranged by size:
588 522 229 116 75 16 11
BbeI G_GCGC'C
Cuts at: 0 136 597 1268 1388 1505 1557
Size: 136 461 671 120 117 52
Fragments arranged by size:
671 461 136 120 117 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 1317 1557
Size: 1317 240
BciVI GTATCCnnnnn_n'
Cuts at: 0 13 1557
Size: 13 1544
BglI GCCn_nnn'nGGC
Cuts at: 0 124 1210 1557
Size: 124 1086 347
Fragments arranged by size:
1086 347 124
BlpI GC'TnA_GC
Cuts at: 0 1091 1557
Size: 1091 466
Bme1580I G_kGCm'C
Cuts at: 0 116 969 1481 1557
Size: 116 853 512 76
Fragments arranged by size:
853 512 116 76
BmgBI CAC'GTC
Cuts at: 0 620 1235 1557
Size: 620 615 322
Fragments arranged by size:
620 615 322
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 1320 1557
Size: 36 1284 237
Fragments arranged by size:
1284 237 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1557
Size: 210 1347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 877 979 1105 1557
Size: 784 93 102 126 452
Fragments arranged by size:
784 452 126 102 93
Bpu10I CC'TnA_GC
Cuts at: 0 67 1153 1557
Size: 67 1086 404
Fragments arranged by size:
1086 404 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 583 700 1168 1557
Size: 454 129 117 468 389
Fragments arranged by size:
468 454 389 129 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 570 1557
Size: 448 122 987
Fragments arranged by size:
987 448 122
BsaAI yAC'GTr
Cuts at: 0 74 258 1557
Size: 74 184 1299
Fragments arranged by size:
1299 184 74
BsaHI Gr'CG_yC
Cuts at: 0 133 212 277 594 1072 1265 1385 1487
Size: 133 79 65 317 478 193 120 102
Cuts at: 1487 1502 1557
Size: 15 55
Fragments arranged by size:
478 317 193 133 120 102 79 65
55 15
BsaWI w'CCGG_w
Cuts at: 0 85 911 1557
Size: 85 826 646
Fragments arranged by size:
826 646 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1270 1300 1557
Size: 13 30 1227 30 257
Fragments arranged by size:
1227 257 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 688 691 694 706 709 721 766
Size: 21 667 3 3 12 3 12 45
Cuts at: 766 1557
Size: 791
Fragments arranged by size:
791 667 45 21 12 12 3 3
3
BseYI C'CCAG_C
Cuts at: 0 207 1050 1419 1491 1557
Size: 207 843 369 72 66
Fragments arranged by size:
843 369 207 72 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1557
Size: 1430 127
BsiEI CG_ry'CG
Cuts at: 0 200 995 1557
Size: 200 795 562
Fragments arranged by size:
795 562 200
BsiHKAI G_wGCw'C
Cuts at: 0 73 191 491 724 1557
Size: 73 118 300 233 833
Fragments arranged by size:
833 300 233 118 73
Bsp1286I G_dGCh'C
Cuts at: 0 73 116 191 491 713 724 740 969
Size: 73 43 75 300 222 11 16 229
Cuts at: 969 1481 1557
Size: 512 76
Fragments arranged by size:
512 300 229 222 76 75 73 43
16 11
BspEI T'CCGG_A
Cuts at: 0 85 911 1557
Size: 85 826 646
Fragments arranged by size:
826 646 85
BsrFI r'CCGG_y
Cuts at: 0 135 161 590 810 1062 1534 1557
Size: 135 26 429 220 252 472 23
Fragments arranged by size:
472 429 252 220 135 26 23
BssHII G'CGCG_C
Cuts at: 0 178 586 905 1557
Size: 178 408 319 652
Fragments arranged by size:
652 408 319 178
BssSI C'ACGA_G
Cuts at: 0 184 1557
Size: 184 1373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 77 1557
Size: 77 1480
BstEII G'GTnAC_C
Cuts at: 0 1293 1557
Size: 1293 264
BstYI r'GATC_y
Cuts at: 0 325 1557
Size: 325 1232
Bsu36I CC'TnA_GG
Cuts at: 0 900 1031 1342 1557
Size: 900 131 311 215
Fragments arranged by size:
900 311 215 131
BtgI C'CryG_G
Cuts at: 0 200 416 628 740 926 1199 1557
Size: 200 216 212 112 186 273 358
Fragments arranged by size:
358 273 216 212 200 186 112
BtsI GCAGTG_nn'
Cuts at: 0 1006 1557
Size: 1006 551
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1557
Size: 886 671
EaeI y'GGCC_r
Cuts at: 0 53 170 197 533 622 923 992 1557
Size: 53 117 27 336 89 301 69 565
Fragments arranged by size:
565 336 301 117 89 69 53 27
EagI C'GGCC_G
Cuts at: 0 197 992 1557
Size: 197 795 565
Fragments arranged by size:
795 565 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 248 270 950 1457 1557
Size: 118 130 22 680 507 100
Fragments arranged by size:
680 507 130 118 100 22
EcoICRI GAG'CTC
Cuts at: 0 189 722 1557
Size: 189 533 835
Fragments arranged by size:
835 533 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 844 877 979 1105 1557
Size: 784 60 33 102 126 452
Fragments arranged by size:
784 452 126 102 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 402 559 601 1528 1557
Size: 402 157 42 927 29
Fragments arranged by size:
927 402 157 42 29
EcoO109I rG'GnC_Cy
Cuts at: 0 223 339 954 965 1195 1557
Size: 223 116 615 11 230 362
Fragments arranged by size:
615 362 230 223 116 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 1557
Size: 849 708
FspI TGC'GCA
Cuts at: 0 332 642 1557
Size: 332 310 915
Fragments arranged by size:
915 332 310
HaeII r_GCGC'y
Cuts at: 0 136 208 597 1191 1268 1388 1505 1557
Size: 136 72 389 594 77 120 117 52
Fragments arranged by size:
594 389 136 120 117 77 72 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1557
Size: 1156 32 369
Fragments arranged by size:
1156 369 32
KasI G'GCGC_C
Cuts at: 0 132 593 1264 1384 1501 1557
Size: 132 461 671 120 117 56
Fragments arranged by size:
671 461 132 120 117 56
MscI TGG'CCA
Cuts at: 0 55 172 535 1557
Size: 55 117 363 1022
Fragments arranged by size:
1022 363 117 55
MslI CAynn'nnrTG
Cuts at: 0 547 1557
Size: 547 1010
MspA1I CmG'CkG
Cuts at: 0 202 355 425 630 795 928 1132 1201
Size: 202 153 70 205 165 133 204 69
Cuts at: 1201 1495 1557
Size: 294 62
Fragments arranged by size:
294 205 204 202 165 153 133 70
69 62
NaeI GCC'GGC
Cuts at: 0 163 592 812 1064 1536 1557
Size: 163 429 220 252 472 21
Fragments arranged by size:
472 429 252 220 163 21
NarI GG'CG_CC
Cuts at: 0 133 594 1265 1385 1502 1557
Size: 133 461 671 120 117 55
Fragments arranged by size:
671 461 133 120 117 55
NcoI C'CATG_G
Cuts at: 0 416 1557
Size: 416 1141
NgoMIV G'CCGG_C
Cuts at: 0 161 590 810 1062 1534 1557
Size: 161 429 220 252 472 23
Fragments arranged by size:
472 429 252 220 161 23
NspI r_CATG'y
Cuts at: 0 1182 1557
Size: 1182 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 843 1557
Size: 843 714
PfoI T'CCnGG_A
Cuts at: 0 1391 1557
Size: 1391 166
PmlI CAC'GTG
Cuts at: 0 74 1557
Size: 74 1483
PpuMI rG'GwC_Cy
Cuts at: 0 223 954 1195 1557
Size: 223 731 241 362
Fragments arranged by size:
731 362 241 223
PshAI GACnn'nnGTC
Cuts at: 0 851 1557
Size: 851 706
PspOMI G'GGCC_C
Cuts at: 0 112 965 1557
Size: 112 853 592
Fragments arranged by size:
853 592 112
PstI C_TGCA'G
Cuts at: 0 485 1500 1557
Size: 485 1015 57
Fragments arranged by size:
1015 485 57
PvuII CAG'CTG
Cuts at: 0 1495 1557
Size: 1495 62
SacI G_AGCT'C
Cuts at: 0 191 724 1557
Size: 191 533 833
Fragments arranged by size:
833 533 191
SacII CC_GC'GG
Cuts at: 0 203 631 929 1202 1557
Size: 203 428 298 273 355
Fragments arranged by size:
428 355 298 273 203
SanDI GG'GwC_CC
Cuts at: 0 223 1557
Size: 223 1334
SexAI A'CCwGG_T
Cuts at: 0 571 1557
Size: 571 986
SfcI C'TryA_G
Cuts at: 0 481 1496 1557
Size: 481 1015 61
Fragments arranged by size:
1015 481 61
SfoI GGC'GCC
Cuts at: 0 134 595 1266 1386 1503 1557
Size: 134 461 671 120 117 54
Fragments arranged by size:
671 461 134 120 117 54
SgrAI Cr'CCGG_yG
Cuts at: 0 590 810 1557
Size: 590 220 747
Fragments arranged by size:
747 590 220
SmaI CCC'GGG
Cuts at: 0 1401 1557
Size: 1401 156
SmlI C'TyrA_G
Cuts at: 0 469 598 715 1183 1557
Size: 469 129 117 468 374
Fragments arranged by size:
469 468 374 129 117
SphI G_CATG'C
Cuts at: 0 1182 1557
Size: 1182 375
StuI AGG'CCT
Cuts at: 0 475 898 1082 1366 1557
Size: 475 423 184 284 191
Fragments arranged by size:
475 423 284 191 184
StyI C'CwwG_G
Cuts at: 0 311 416 512 1191 1379 1529 1557
Size: 311 105 96 679 188 150 28
Fragments arranged by size:
679 311 188 150 105 96 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1302 1557
Size: 1302 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1557
Size: 1270 32 255
Fragments arranged by size:
1270 255 32
Tth111I GACn'n_nGTC
Cuts at: 0 1321 1557
Size: 1321 236
XmaI C'CCGG_G
Cuts at: 0 1399 1557
Size: 1399 158
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI
BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI
DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BglII BmtI BsaBI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BsrGI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII EarI EcoRI EcoRV FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SfiI SgfI SnaBI SpeI SrfI SspI
SwaI TatI XbaI XcmI XhoI XmnI ZraI